{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:53:20Z","timestamp":1791572000747,"version":"4.3.4"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National Institute for Food and Agriculture Research Initiative Competitive","award":["2016-67015-24958"],"award-info":[{"award-number":["2016-67015-24958"]}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agriculture and Food Research Initiative Competitive","award":["2016-67015-24958"],"award-info":[{"award-number":["2016-67015-24958"]}]},{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Tissue-nonspecific alkaline phosphatase (TNSALP; encoded by ALPL gene) has a critical role in the regulation of phosphate homeostasis postnatally. However, the utero-placental expression of TNSALP and the role in phosphate transport in pregnancy is poorly understood. Estrous cycles of ewes were synchronized, and ewes were euthanized and hysterectomized on Days 1, 9, or 14 of the estrous cycle or bred to fertile rams and euthanized and hysterectomized on Days 9, 12, 17, 30, 50, 70, 90, 110, or 125 of pregnancy. The expression of ALPL mRNA, immunolocalization of TNSALP protein, and quantification and localization of TNSALP enzymatic activity was performed on ovine endometria and placentomes. Day of the estrous cycle did not alter ALPL mRNA expression or enzymatic activity of TNSALP. TNSALP protein localized to uterine epithelial and stromal cells, blood vessels, myometrium, caruncular, and cotyledonary stroma. TNSALP activity was localized to uterine epithelia, blood vessels, caruncular stroma (from Day 70 of gestation), and the apical surface of chorionic epithelia (from Day 50 of gestation). TNSALP protein and activity localized to the apical surface of uterine epithelia during the estrous cycle and in early pregnancy. Endometrial TNSALP enzymatic activity was downregulated on Days 17 and 30 of gestation (P\u00a0&amp;lt;\u20090.05). Expression of ALPL mRNA decreased in late gestation in endometria and placentomes (P\u00a0&amp;lt;\u20090.05). TNSALP activity peaked in placentomes on Days 70 and 90 of gestation. Collectively, these results suggest a potential role of TNSALP in the regulation of phosphate transport and homeostasis at the maternal\u2013conceptus interface in ruminants.<\/jats:p>","DOI":"10.1093\/biolre\/ioad113","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T14:50:14Z","timestamp":1694098214000},"page":"954-964","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of TNSALP expression, localization, and activity in ovine utero-placental tissues"],"prefix":"10.1093","volume":"109","author":[{"given":"Claire","family":"Stenhouse","sequence":"first","affiliation":[{"name":"Department of Animal Science, Pennsylvania State University , University Park, Pennsylvania , USA"},{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine M","family":"Halloran","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makenzie G","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn M","family":"Moses","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirvay","family":"Sah","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry J","family":"Suva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology and Pharmacology, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Gaddy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Integrative Biosciences, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuller W","family":"Bazer","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&M University , College Station, Texas , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"2024090700052323900_ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fped.2021.600490","article-title":"Endocrine diseases of newborn: epidemiology, pathogenesis, therapeutic options, and outcome \u201ccurrent insights into disorders of calcium and phosphate in the newborn\u201d","volume":"9","author":"Taylor-Miller","year":"2021","journal-title":"Front Pediatr"},{"key":"2024090700052323900_ref2","volume-title":"Principles of Animal Nutrition","author":"Wu","year":"2018"},{"key":"2024090700052323900_ref3","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1642-431X(12)60012-6","article-title":"Interferons and progesterone for establishment and maintenance of pregnancy: interactions among novel cell signaling pathways","volume":"8","author":"Bazer","year":"2008","journal-title":"Reprod Biol"},{"key":"2024090700052323900_ref4","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1093\/biolre\/ioab028","article-title":"Novel mineral regulatory pathways in ovine pregnancy: 1. 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