{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:18:41Z","timestamp":1790849921827,"version":"4.1.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T00:00:00Z","timestamp":1695427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Karolinska Institutet\u2019s Research Foundations"},{"name":"Dementia Foundation"},{"name":"Olle Engkvist Byggm\u00e4stare Foundation"},{"DOI":"10.13039\/501100005701","name":"\u00c5hl\u00e9n-Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005701","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Magnus Bergvalls Foundation"},{"DOI":"10.13039\/100009673","name":"Gun and Bertil Stohnes Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009673","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010815","name":"Foundation for Old Servants","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010815","id-type":"DOI","asserted-by":"publisher"}]},{"name":"ALF-agreement","award":["20200330"],"award-info":[{"award-number":["20200330"]}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"publisher","award":["2016-01806"],"award-info":[{"award-number":["2016-01806"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Little is known about the non-neuronal spermic cholinergic system, which may regulate sperm motility and the acrosome reaction initiation process. We investigated the presence of the key acetylcholine (ACh)-biosynthesizing enzyme, choline acetyltransferase (ChAT), and the acetylcholine-degrading enzymes, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) and two ACh-receptors in human spermatozoa and seminal plasma. Fresh ejaculates were used for intra- and extracellular flow cytometric analysis of ChAT, AChE, BChE, and alpha-7-nicotinic and M1-muscarinic ACh-receptors in sperm. For determining the source of soluble enzymes, frozen seminal samples (n\u2009=\u200974) were selected on two bases: (1) from vasectomized (n\u2009=\u200937) and non-vasectomized (n\u2009=\u200937) subjects and (2) based on levels of alpha-glucosidase, fructose, or zinc to define sample subgroups with high or low fluid contribution from the epididymis and seminal vesicle, and prostate, respectively. Flow cytometric analyses revealed that ChAT was expressed intracellularly in essentially all spermatozoa. ChAT was also present in a readily membrane-detachable form at the extracellular membrane of at least 18% of the spermatozoa. These were also highly positive for intra- and extracellular BChE (&amp;gt;83%) and M1 (&amp;gt;84%) and \u03b17 (&amp;gt;59%) ACh-receptors. Intriguingly, the sperm was negative for AChE. Analyses of seminal plasma revealed that spermatozoa and epididymides were major sources of soluble ChAT and BChE, whereas soluble AChE most likely originated from epididymides and seminal vesicles. Prostate had relatively minor contribution to the pool of the soluble enzymes in the seminal fluid. In conclusion, human spermatozoa exhibited a cholinergic phenotype and were one of the major sources of soluble ChAT and BChE in ejaculate. We also provide the first evidence for ChAT as an extracellularly membrane-anchored protein.<\/jats:p>","DOI":"10.1093\/biolre\/ioad127","type":"journal-article","created":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:57:18Z","timestamp":1695506238000},"page":"63-77","source":"Crossref","is-referenced-by-count":4,"title":["Presence of key cholinergic enzymes in human spermatozoa and seminal fluid"],"prefix":"10.1093","volume":"110","author":[{"given":"Banita","family":"Thakur","sequence":"first","affiliation":[{"name":"Department of Neurobiology , Care Sciences and Society, Division of Clinical Geriatrics, , Stockholm , Sweden"},{"name":"Karolinska Institutet , Care Sciences and Society, Division of Clinical Geriatrics, , Stockholm , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila Pamela","family":"Hasooni","sequence":"additional","affiliation":[{"name":"Department of Neurobiology , Care Sciences and Society, Division of Clinical Geriatrics, , 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Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Bj\u00f6rndahl","sequence":"additional","affiliation":[{"name":"ANOVA, Karolinska University Hospital , Stockholm , Sweden"},{"name":"Department of Medicine Huddinge, Karolinska Institutet , Stockholm , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taher","family":"Darreh-Shori","sequence":"additional","affiliation":[{"name":"Department of Neurobiology , Care Sciences and Society, Division of Clinical Geriatrics, , Stockholm , Sweden"},{"name":"Karolinska Institutet , Care Sciences and Society, Division of Clinical Geriatrics, , Stockholm , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,23]]},"reference":[{"key":"2024011612151914800_ref1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1254\/jjp.85.2","article-title":"The biological role of non-neuronal acetylcholine in plants and 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