{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:28:31Z","timestamp":1783488511896,"version":"3.55.0"},"reference-count":128,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T00:00:00Z","timestamp":1703894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["#K99HD103778"],"award-info":[{"award-number":["#K99HD103778"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["#R00HD103778"],"award-info":[{"award-number":["#R00HD103778"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"NIH\/NCATS Colorado CTSA","award":["#T32TR004367"],"award-info":[{"award-number":["#T32TR004367"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>New microscopy techniques in combination with tissue clearing protocols and emerging analytical approaches have presented researchers with the tools to understand dynamic biological processes in a three-dimensional context. This paves the road for the exploration of new research questions in reproductive biology, for which previous techniques have provided only approximate resolution. These new methodologies now allow for contextualized analysis of far-larger volumes than was previously possible. Tissue optical clearing and three-dimensional imaging techniques posit the bridging of molecular mechanisms, macroscopic morphogenic development, and maintenance of reproductive function into one cohesive and comprehensive understanding of the biology of the reproductive system. In this review, we present a survey of the various tissue clearing techniques and imaging systems, as they have been applied to the developing and adult reproductive system. We provide an overview of tools available for analysis of experimental data, giving particular attention to the emergence of artificial intelligence\u2013assisted methods and their applicability to image analysis. We conclude with an evaluation of how novel image analysis approaches that have been applied to other organ systems could be incorporated into future experimental evaluation of reproductive biology.<\/jats:p>","DOI":"10.1093\/biolre\/ioad182","type":"journal-article","created":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T15:26:11Z","timestamp":1703949971000},"page":"1041-1054","source":"Crossref","is-referenced-by-count":6,"title":["Tissue clearing and imaging approaches for in toto analysis of the reproductive system"],"prefix":"10.1093","volume":"110","author":[{"given":"Lillian","family":"Folts","sequence":"first","affiliation":[{"name":"Section of Developmental Biology , Department of Pediatrics, , Aurora CO , USA"},{"name":"University of Colorado Anschutz Medical Campus , Department of Pediatrics, , Aurora CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony S","family":"Martinez","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology , Department of Pediatrics, , Aurora CO , USA"},{"name":"University of Colorado Anschutz Medical Campus , Department of Pediatrics, , Aurora CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"McKey","sequence":"additional","affiliation":[{"name":"Section of Developmental Biology , Department of Pediatrics, , Aurora CO , USA"},{"name":"University of Colorado Anschutz Medical Campus , Department of Pediatrics, , Aurora CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,30]]},"reference":[{"key":"2024061704412692900_ref1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.media.2018.02.004","article-title":"A survey of methods for 3D histology reconstruction","volume":"46","author":"Pichat","year":"2018","journal-title":"Med Image Anal"},{"key":"2024061704412692900_ref2","volume-title":"Ueber das Durchsichtigmachen von menschlichen und 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