{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:59:31Z","timestamp":1780343971858,"version":"3.54.1"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Colorado Center for Reproductive Medicine"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chemically defined oocyte maturation media supplemented with FGF2, LIF, and IGF-1 (FLI medium) enabled significantly improved oocyte quality in multiple farm animals, yet the molecular mechanisms behind such benefits were poorly defined. Here, we first demonstrated that FLI medium enhanced mouse oocyte quality assessed by blastocyst formation after in vitro fertilization and implantation and fetal development after embryo transfer. We then analyzed the glucose concentrations in the spent media; reactive oxygen species concentrations; mitochondrial membrane potential; spindle morphology in oocytes; and the abundance of transcripts of endothelial growth factor\u2013like factors, cumulus expansion factors, and glucose metabolism\u2013related genes in cumulus cells. We found that FLI medium enabled increased glucose metabolism through glycolysis, pentose phosphate pathway, and hexosamine biosynthetic pathway, as well as more active endothelial growth factor\u2013like factor expressions in cumulus cells, resulting in improved cumulus cell expansion, decreased spindle abnormality, and overall improvement in oocyte quality. In addition, the activities of MAPK1\/3, PI3K\/AKT, JAK\/STAT3, and mTOR signaling pathways in cumulus cells were assessed by the phosphorylation of MAPK1\/3, AKT, STAT3, and mTOR downstream target RPS6KB1. We demonstrated that FLI medium promoted activations of all these signaling pathways at multiple different time points during in vitro maturation.<\/jats:p>","DOI":"10.1093\/biolre\/ioae014","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T02:39:41Z","timestamp":1706063981000},"page":"672-683","source":"Crossref","is-referenced-by-count":15,"title":["FGF2, LIF, and IGF-1 supplementation improves mouse oocyte in vitro maturation via increased glucose metabolism"],"prefix":"10.1093","volume":"110","author":[{"given":"Asrafun","family":"Nahar","sequence":"first","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Becker","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolando","family":"Pasquariello","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"},{"name":"Department of Agricultural and Environmental Sciences, University of Milan , Milan , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Herrick","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"},{"name":"Omaha\u2019s Henry Doorly Zoo and Aquarium , Omaha, NE , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Rogers","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Schoolcraft","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L","family":"Krisher","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"},{"name":"Genus Plc , DeForest, WI , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Yuan","sequence":"additional","affiliation":[{"name":"Colorado Center for Reproductive Medicine , Lone Tree, CO , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"2024041508363970500_ref1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/978-1-61779-436-0_14","article-title":"In vitro maturation (IVM) of porcine 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