{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:14:49Z","timestamp":1787008489999,"version":"build-2736575974"},"reference-count":112,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T00:00:00Z","timestamp":1707350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"NCATS","doi-asserted-by":"publisher","award":["1UH2TR00411701"],"award-info":[{"award-number":["1UH2TR00411701"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Research on the biology of fetal\u2013maternal barriers has been limited by access to physiologically relevant cells, including trophoblast cells. In this study, we describe the development of a human term placenta\u2013derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. Human-term placenta\u2013derived cytotrophoblast immortalized cell line cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts. We also compared the hPTCCTB cells to immortalized chorionic trophoblasts (hFM-CTC), trophoblasts of the chorionic plate, and BeWo cells, choriocarcinoma cell lines of conventional use. Human-term placenta\u2013derived cytotrophoblast immortalized cell line and hFM-CTCs displayed more similarity to each other than to BeWos, but these differ in syncytialization ability. Overall, this study (1) demonstrates that the immortalized hPTCCTB generated are cells of higher physiological relevance and (2) provides a look into the distinction between the spatially distinct placental and fetal barrier trophoblasts cells, hPTCCTB and hFM-CTC, respectively.<\/jats:p>","DOI":"10.1093\/biolre\/ioae026","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T17:50:54Z","timestamp":1707241854000},"page":"950-970","source":"Crossref","is-referenced-by-count":14,"title":["Establishment and comparison of human term placenta\u2013derived trophoblast cells"],"prefix":"10.1093","volume":"110","author":[{"suffix":"Jr","given":"Manuel S","family":"Vidal","sequence":"first","affiliation":[{"name":"Division of Basic Science and Translational Research , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"Department of Biochemistry and Molecular Biology , College of Medicine, , Manila ,","place":["Philippines"]},{"name":"University of the Philippines Manila , College of Medicine, , Manila ,","place":["Philippines"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enkhtuya","family":"Radnaa","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Vora","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil","family":"Khanipov","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology , , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , , Galveston, TX ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Antich","sequence":"additional","affiliation":[{"name":"3D Tissue Bioprinting Laboratory , National Center for Advancing Translational Sciences, , Bethesda, MD ,","place":["USA"]},{"name":"National Institute of Sciences , National Center for Advancing Translational Sciences, , Bethesda, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Ferrer","sequence":"additional","affiliation":[{"name":"3D Tissue Bioprinting Laboratory , National Center for Advancing Translational Sciences, , Bethesda, MD ,","place":["USA"]},{"name":"National Institute of Sciences , National Center for Advancing Translational Sciences, , Bethesda, MD ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rheanna","family":"Urrabaz-Garza","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeena E","family":"Jacob","sequence":"additional","affiliation":[{"name":"Division of Basic Science and Translational Research , Department of Obstetrics and Gynaecology, , Galveston, TX ,","place":["USA"]},{"name":"The University of Texas Medical Branch at Galveston , Department of Obstetrics and Gynaecology, , Galveston, TX 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