{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:14:49Z","timestamp":1787008489827,"version":"build-2736575974"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to evaluate the role of angiotensin-converting enzyme 1 (ACE1) in H2O2-induced trophoblast cell injury and the potential molecular mechanisms. Oxidative stress was modeled by exposing HTR-8\/SVneo cells to 200\u00a0\u03bcM H2O2. Western blot and real-time quantitative PCR methods were used to detect protein and mRNA expression level of ACE1 in chorionic villus tissue and trophoblast HTR-8\/SVneo cell. Inhibition of ACE1 expression was achieved by transfection with small interfering RNA. Then flow cytometry, Cell Counting Kit-8, and Transwell assay was used to assess apoptosis, viability, and migration ability of the cells. Reactive oxygen species (ROS) were detected by fluorescent probes, and malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) activities were determined by corresponding detection kits. Angiotensin-converting enzyme 1 expression was upregulated in chorionic villus tissue of patients with missed abortion (MA) compared with individuals with normal early pregnancy abortion. H2O2 induced elevated ACE1 expression in HTR-8\/SVneo cells, promoted apoptosis, and inhibited cell viability and migration. Knockdown of ACE1 expression inhibited H2O2-induced effects to enhance cell viability and migration and suppress apoptosis. Additionally, H2O2 stimulation caused increased levels of ROS and MDA and decreased SOD and GSH activity in the cells, whereas knockdown of ACE1 expression led to opposite changes of these oxidative stress indicators. Moreover, knockdown of ACE1 attenuated the inhibitory effect of H2O2 on the Nrf2\/HO-1 pathway. Angiotensin-converting enzyme 1 was associated with MA, and it promoted H2O2-induced injury of trophoblast cells through inhibiting the Nrf2 pathway. Therefore, ACE1 may serve as a potential therapeutic target for MA.<\/jats:p>","DOI":"10.1093\/biolre\/ioae049","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T03:57:21Z","timestamp":1711771041000},"page":"406-413","source":"Crossref","is-referenced-by-count":3,"title":["Association between ACE1 and missed abortion: ACE1 promotes H2O2-induced trophoblast cell injury in vitro"],"prefix":"10.1093","volume":"111","author":[{"given":"Yi-dan","family":"Yan","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology, Changshu Hospital of Traditional Chinese Medicine , Changshu, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-xia","family":"Ji","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology, Changshu Hospital of Traditional Chinese Medicine , Changshu, Jiangsu , 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