{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:21:48Z","timestamp":1792596108928,"version":"4.5.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD109152"],"award-info":[{"award-number":["R01HD109152"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32HD087166"],"award-info":[{"award-number":["T32HD087166"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"MSU AgBio Research"},{"DOI":"10.13039\/100007709","name":"Michigan State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007709","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Uterine muscle contractility is essential for reproductive processes including sperm and embryo transport, and during the uterine cycle to remove menstrual effluent. Even still, uterine contractions have primarily been studied in the context of preterm labor. This is partly due to a lack of methods for studying the uterine muscle contractility in the intact organ. Here, we describe an imaging-based method to evaluate mouse uterine contractility of both the longitudinal and circular muscles in the cycling stages and in early pregnancy. By transforming the image-based data into three-dimensional spatiotemporal contractility maps, we calculate waveform characteristics of muscle contractions, including amplitude, frequency, wavelength, and velocity. We report that the native organ is highly contractile during the progesterone-dominant diestrus stage of the cycle when compared to the estrogen-dominant proestrus and estrus stages. We also observed that during the first phase of uterine embryo movement when clustered embryos move toward the middle of the uterine horn, contractions are dynamic and non-uniform between different segments of the uterine horn. In the second phase of embryo movement, contractions are more uniform and rhythmic throughout the uterine horn. Finally, in Lpar3\u2212\/\u2212 uteri, which display faster embryo movement, we observe global and regional increases in contractility. Our method provides a means to understand the wave characteristics of uterine smooth muscle in response to modulators and in genetic mutants. Better understanding uterine contractility in the early pregnancy stages is critical for the advancement of artificial reproductive technologies and a possibility of modulating embryo movement during clinical embryo transfers.<\/jats:p>","DOI":"10.1093\/biolre\/ioae071","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T17:23:34Z","timestamp":1715102614000},"page":"1175-1190","source":"Crossref","is-referenced-by-count":8,"title":["Imaging the dynamics of murine uterine contractions in early pregnancy"],"prefix":"10.1093","volume":"110","author":[{"given":"Madeline","family":"Dawson","sequence":"first","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Flores","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Anandasenthil","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Mahesh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olmo","family":"Zavala-Romero","sequence":"additional","affiliation":[{"name":"Department of Scientific Computing, Florida State University , Tallahassee, Florida , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ripla","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Obstetrics , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Michigan State University , Gynecology and Reproductive Biology, , East Lansing, Michigan , USA"},{"name":"Institute for Quantitative Health Science and Engineering, Michigan State University , East Lansing, Michigan , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,7]]},"reference":[{"key":"2024061704455575500_ref1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1152\/advan.00025.2003","article-title":"Smooth muscle contraction and relaxation","volume":"27","author":"Webb","year":"2003","journal-title":"Adv Physiol Educ"},{"key":"2024061704455575500_ref2","author":"Hafen","year":"2023"},{"key":"2024061704455575500_ref3","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1152\/physrev.00038.2003","article-title":"Urinary bladder contraction and relaxation: physiology and pathophysiology","volume":"84","author":"Andersson","year":"2004","journal-title":"Physiol Rev"},{"key":"2024061704455575500_ref4","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1093\/humupd\/dmq016","article-title":"Physiological pathways and molecular mechanisms regulating uterine contractility","volume":"16","author":"Aguilar","year":"2010","journal-title":"Hum Reprod Update"},{"key":"2024061704455575500_ref5","volume-title":"Comparative Morphology of the Endometrium","author":"Massman, Harland","year":"1980"},{"key":"2024061704455575500_ref6","doi-asserted-by":"crossref","first-page":"8274","DOI":"10.1038\/s41598-020-65299-0","article-title":"A novel third mesh-like myometrial layer connects the longitudinal and circular muscle fibers\u2014a potential stratum to coordinate uterine contractions","volume":"10","author":"Kagami","year":"2020","journal-title":"Sci Rep"},{"key":"2024061704455575500_ref7","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1530\/REP-17-0647","article-title":"Monitoring uterine contractility in mice using a transcervical intrauterine pressure catheter","volume":"155","author":"Robuck","year":"2018","journal-title":"Reproduction"},{"key":"2024061704455575500_ref8","first-page":"467","article-title":"Effects of interactions between progesterone and prostaglandin on uterine contractility in a perfused swine uterus model","volume":"28","author":"K\u00fcnzel","year":"2014","journal-title":"In Vivo"},{"key":"2024061704455575500_ref9","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1097\/01.gco.0000233947.97543.c4","article-title":"Uterine contractility and embryo implantation","volume":"18","author":"Bulletti","year":"2006","journal-title":"Curr Opin Obstet Gynecol"},{"key":"2024061704455575500_ref10","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1111\/1754-9485.12760","article-title":"Transient uterine contractions as a potential pathology mimic on premenopausal pelvic MRI and the role of routine repeat T2 sagittal images to improve observer confidence","volume":"62","author":"Lam","year":"2018","journal-title":"J Med Imaging Radiat Oncol"},{"key":"2024061704455575500_ref11","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/0041-624X(78)90055-0","article-title":"Ultrasonically induced contractions in mouse uterine smooth muscle in vivo","volume":"16","author":"Ter Haar","year":"1978","journal-title":"Ultrasonics"},{"key":"2024061704455575500_ref12","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2021.744745","article-title":"Mode switch of Ca(2 +) oscillation-mediated uterine peristalsis and associated embryo implantation impairments in mouse Adenomyosis","volume":"12","author":"Qu","year":"2021","journal-title":"Front Physiol"},{"key":"2024061704455575500_ref13","first-page":"363","article-title":"Increase of the uterus-relaxant effect of nifedipine by the Abcg2 efflux protein inhibitor KO134 in the rat in vivo","volume":"27","author":"Lovasz","year":"2013","journal-title":"In Vivo"},{"key":"2024061704455575500_ref14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1095\/biolreprod.115.129809","article-title":"Uterine contractility in the nonpregnant mouse: changes during the Estrous cycle and effects of Chloride Channel blockade","volume":"92","author":"Dodds","year":"2015","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref15","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1477-7827-5-41","article-title":"The role of voltage-gated potassium channels in the regulation of mouse uterine contractility","volume":"5","author":"Smith","year":"2007","journal-title":"Reprod Biol Endocrinol"},{"key":"2024061704455575500_ref16","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1095\/biolreprod.107.066373","article-title":"Uterine contractions depend on KIT-positive interstitial cells in the mouse: genetic and pharmacological evidence","volume":"79","author":"Allix","year":"2008","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.medici.2017.05.002","article-title":"A role of alpha-tocopherol and phylloquinone in the modulation of uterine contractility and reproductive function in mouse models","volume":"53","author":"Bafor","year":"2017","journal-title":"Medicina (Kaunas)"},{"key":"2024061704455575500_ref18","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1530\/jrf.0.0600053","article-title":"Myoelectrical activity and transport of unfertilized ova in the oviduct of the mouse in vitro","volume":"60","author":"Talo","year":"1980","journal-title":"J Reprod Fertil"},{"key":"2024061704455575500_ref19","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1095\/biolreprod61.4.873","article-title":"Maturation of spontaneous and agonist-induced uterine contractions in the peripartum mouse uterus","volume":"61","author":"Mackler","year":"1999","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1566-0702(02)00233-3","article-title":"Regional and muscle layer variations in cholinergic nerve control of the rat myometrium during the oestrous cycle","volume":"104","author":"Houdeau","year":"2003","journal-title":"Auton Neurosci"},{"key":"2024061704455575500_ref21","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1071\/RD9910185","article-title":"In vivo myometrial activity in the rat during the oestrous cycle: studies with a novel technique of video laparoscopy","volume":"3","author":"Crane","year":"1991","journal-title":"Reprod Fertil Dev"},{"key":"2024061704455575500_ref22","doi-asserted-by":"crossref","first-page":"R317","DOI":"10.1152\/ajpregu.00327.2020","article-title":"Identification of a novel distension-evoked motility pattern in the mouse uterus","volume":"321","author":"Dodds","year":"2021","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"2024061704455575500_ref23","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1177\/1933719117715122","article-title":"The effects of platelet-activating factor on uterine contractility, perfusion, hypoxia, and pain in mice","volume":"25","author":"Hellman","year":"2018","journal-title":"Reprod Sci"},{"key":"2024061704455575500_ref24","doi-asserted-by":"crossref","first-page":"20180082","DOI":"10.1098\/rsfs.2018.0082","article-title":"Analysis of in vivo uterine peristalsis in the non-pregnant female mouse","volume":"9","author":"Zhang","year":"2019","journal-title":"Interface Focus"},{"key":"2024061704455575500_ref25","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1242\/dev.193490","article-title":"Mechanical and signaling mechanisms that guide pre-implantation embryo movement","volume":"147","author":"Flores","year":"2020","journal-title":"Development"},{"key":"2024061704455575500_ref26","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1095\/biolreprod.108.073833","article-title":"Chlamydia infection causes loss of pacemaker cells and inhibits oocyte transport in the mouse oviduct","volume":"80","author":"Dixon","year":"2009","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref27","doi-asserted-by":"crossref","first-page":"R25","DOI":"10.1530\/REP-22-0250","article-title":"Dynamics of gametes and embryos in the oviduct: what can in vivo imaging reveal?","volume":"165","author":"Wang","year":"2023","journal-title":"Reproduction"},{"key":"2024061704455575500_ref28","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1095\/biolreprod.107.060293","article-title":"Embryo spacing and implantation timing are differentially regulated by LPA3-mediated lysophosphatidic acid signaling in mice","volume":"77","author":"Hama","year":"2007","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref29","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/nature03505","article-title":"LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing","volume":"435","author":"Ye","year":"2005","journal-title":"Nature"},{"key":"2024061704455575500_ref30","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/dvg.20335","article-title":"A global double-fluorescent Cre reporter mouse","volume":"45","author":"Muzumdar","year":"2007","journal-title":"Genesis"},{"key":"2024061704455575500_ref31","first-page":"e4389","article-title":"Performing vaginal lavage, crystal violet staining, and vaginal cytological evaluation for mouse estrous cycle staging identification","volume":"67","author":"McLean","year":"2012","journal-title":"J Vis Exp"},{"key":"2024061704455575500_ref32","doi-asserted-by":"crossref","first-page":"L852","DOI":"10.1152\/ajplung.00130.2004","article-title":"KCl evokes contraction of airway smooth muscle via activation of RhoA and rho-kinase","volume":"287","author":"Janssen","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2024061704455575500_ref33","article-title":"Learning OpenCV - computer vision with the OpenCV library: software that sees","author":"Bradski"},{"key":"2024061704455575500_ref34","first-page":"62","article-title":"The trough-and-ridge diagram","volume":"1","author":"Ernest","year":"1949","journal-title":"Tellus"},{"key":"2024061704455575500_ref35","doi-asserted-by":"crossref","first-page":"4749","DOI":"10.1242\/dev.144386","article-title":"Insights from imaging the implanting embryo and the uterine environment in three dimensions","volume":"143","author":"Arora","year":"2016","journal-title":"Development"},{"key":"2024061704455575500_ref36","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/1471-2105-7-123","article-title":"Detecting outliers when fitting data with nonlinear regression\u2014a new method based on robust nonlinear regression and the false discovery rate","volume":"7","author":"Motulsky","year":"2006","journal-title":"BMC Bioinformatics"},{"key":"2024061704455575500_ref37","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1530\/jrf.0.0170069","article-title":"Increase in length of the uterus at the time of implantation in the mouse","volume":"17","author":"Finn","year":"1968","journal-title":"J Reprod Fertil"},{"key":"2024061704455575500_ref38","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1038\/s41467-021-21512-w","article-title":"Control of oviductal fluid flow by the G-protein coupled receptor Adgrd1 is essential for murine embryo transit","volume":"12","author":"Bianchi","year":"2021","journal-title":"Nat Commun"},{"key":"2024061704455575500_ref39","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2826.2008.01665.x","article-title":"Sex hormones and excitation-contraction coupling in the uterus: the effects of oestrous and hormones","volume":"20","author":"Wray","year":"2008","journal-title":"J Neuroendocrinol"},{"key":"2024061704455575500_ref40","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/0002-9378(78)90852-9","article-title":"Electrical and mechanical activity of rat uterus in vivo during the estrous cycle","volume":"132","author":"Ishikawa","year":"1978","journal-title":"Am J Obstet Gynecol"},{"key":"2024061704455575500_ref41","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1677\/joe.1.06466","article-title":"Effect of the oestrous cycle, pregnancy and uterine region on the responsiveness of the isolated mouse uterus to prostaglandin F(2alpha) and the thromboxane mimetic U46619","volume":"188","author":"Griffiths","year":"2006","journal-title":"J Endocrinol"},{"key":"2024061704455575500_ref42","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1111\/j.1748-1716.1976.tb10289.x","article-title":"Electric activity of the rat myometrium in vivo during the estrous cycle","volume":"97","author":"Talo","year":"1976","journal-title":"Acta Physiol Scand"},{"key":"2024061704455575500_ref43","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1071\/RD9910519","article-title":"Pace-maker activity in the myometrium of the oestrous rat: in vivo studies using video-laparoscopy","volume":"3","author":"Crane","year":"1991","journal-title":"Reprod Fertil Dev"},{"key":"2024061704455575500_ref44","doi-asserted-by":"crossref","first-page":"pgac155","DOI":"10.1093\/pnasnexus\/pgac155","article-title":"Myometrial progesterone receptor determines a transcription program for uterine remodeling and contractions during pregnancy","volume":"1","author":"Wu","year":"2022","journal-title":"PNAS Nexus"},{"key":"2024061704455575500_ref45","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2011643118","article-title":"Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility","volume":"118","author":"Peavey","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2024061704455575500_ref46","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/biolre\/ioab209","article-title":"Intrinsic pacemaker activity and propulsive forces provided by the myosalpinx are necessary for egg and embryo transport in the oviduct","volume":"106","author":"Ward","year":"2022","journal-title":"Biol Reprod"},{"key":"2024061704455575500_ref47","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/j.1469-7793.1999.0575t.x","article-title":"Quantitative analysis of peristalsis in the Guinea-pig small intestine using spatio-temporal maps","volume":"517","author":"Hennig","year":"1999","journal-title":"J Physiol"},{"key":"2024061704455575500_ref48","article-title":"Mouse in utero electroporation: controlled spatiotemporal gene transfection","volume":"54","author":"Matsui","year":"2011","journal-title":"J Vis Exp"},{"key":"2024061704455575500_ref49","doi-asserted-by":"crossref","first-page":"8","DOI":"10.7554\/eLife.42288","article-title":"KymoButler, a deep learning software for automated kymograph analysis","volume":"8","author":"Jakobs","year":"2019","journal-title":"elife"},{"key":"2024061704455575500_ref50","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1262\/jrd.2023-011","article-title":"Lysophosphatidic acid stimulates rat uterine contraction in vitro","volume":"69","author":"Nagashima","year":"2023","journal-title":"J Reprod Dev"},{"key":"2024061704455575500_ref51","first-page":"74","article-title":"Stimulatory effect of lysophosphatidic acids on uterine smooth muscles of non-pregnant rats","volume":"245","author":"Tokumura","year":"1980","journal-title":"Arch Int Pharmacodyn Ther"},{"key":"2024061704455575500_ref52","doi-asserted-by":"crossref","first-page":"635","DOI":"10.2478\/v10181-012-0100-9","article-title":"Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-alpha-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs","volume":"15","author":"Markiewicz","year":"2012","journal-title":"Pol J Vet Sci"},{"key":"2024061704455575500_ref53","first-page":"85","volume-title":"7 - Reproductive Tract Changes During the Mouse Estrous Cycle","author":"Kalyne","year":"2014"},{"key":"2024061704455575500_ref54","doi-asserted-by":"crossref","DOI":"10.1093\/molehr\/gaac043","article-title":"Pre-implantation mouse embryo movement under hormonally altered conditions","volume":"29","author":"Lufkin","year":"2023","journal-title":"Mol Hum Reprod"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioae071\/57824090\/ioae071.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/110\/6\/1175\/58219288\/ioae071.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/110\/6\/1175\/58219288\/ioae071.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T04:46:44Z","timestamp":1718599604000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/110\/6\/1175\/7666628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,7]]},"references-count":54,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,5,7]]},"published-print":{"date-parts":[[2024,6,12]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioae071","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,6]]},"published":{"date-parts":[[2024,5,7]]}}}