{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T15:36:57Z","timestamp":1781537817074,"version":"3.54.5"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Postgraduate Research & Practice Innovation Program of Jiangsu Province","award":["KYCX21_3289"],"award-info":[{"award-number":["KYCX21_3289"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In male reproductive system, proteins containing the coiled-coil domain (CCDC) are predominantly expressed in specific regions including the testis, epididymis, seminal vesicle, and prostate. They play a vital role in centriole formation, sperm motility and flagellar development in male gametes. Despite being highly expressed in the testis, the exact physiological function of the coiled-coil domain-containing 189 (Ccdc189) gene remain largely unclear. Our research provides a comprehensive and detailed investigation into the localization of CCDC189 protein within the testis seminiferous tubules. CCDC189 specifically expressed in spermatocytes, round spermatids, and elongating spermatids in mouse testis. The deletion of Ccdc189 in mouse leads to male infertility, characterized by significantly reduced sperm counts and motility. Abnormally shaped spermatozoa with irregular tails, exhibiting shortened and twisted morphology, were observed in the seminiferous tubules. Electron microscopy revealed disordered and missing peripheral microtubule doublets (MTD) and outer dense fibers (ODF) in the sperm flagella, accompanied by a consistent absence of central pairs (CP). The knockout of Ccdc189 resulted in oligo-astheno-teratozoospermia, which is characterized by low sperm count and reduced sperm motility and abnormal morphology. Furthermore, we identified poly(A)-binding protein cytoplasmic 1 (PABPC1) and PABPC2 as interacting proteins with CCDC189. These proteins belong to the PABP family and are involved in regulating mRNA translational activity in spermatogenic cells by specifically binding to poly(A) tails at the 3\u2032 ends of mRNAs.<\/jats:p>","DOI":"10.1093\/biolre\/ioae112","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T16:49:03Z","timestamp":1721234943000},"page":"800-814","source":"Crossref","is-referenced-by-count":6,"title":["CCDC189 depletion leads to oligo-astheno-teratozoospermia and male infertility in mice"],"prefix":"10.1093","volume":"111","author":[{"given":"Huiping","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Department of Obstetrics and Gynecology , Affiliated Hospital, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , Affiliated Hospital, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changmin","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , School of Medicine, , Yangzhou ,","place":["China"]},{"name":"Department of Obstetrics and Gynecology , Affiliated Hospital, , Yangzhou ,","place":["China"]},{"name":"Yangzhou University , Affiliated Hospital, , Yangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"key":"2024101506321136700_ref1","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.3390\/ijms21051728","article-title":"Molecular biology of spermatogenesis: novel targets of apparently idiopathic male infertility","volume":"21","author":"Cannarella","year":"2020","journal-title":"Int J Mol Sci"},{"key":"2024101506321136700_ref2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1093\/biolre\/ioad001","article-title":"Actin-related protein actl7B ablation leads to OAT with multiple morphological abnormalities of the flagellum and male infertility in mice\u2020","volume":"108","author":"Clement","year":"2023","journal-title":"Biol 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aggregation propensity of intrinsically disordered coiled-coil domain-containing 124","volume":"90","author":"Tuzlako\u011flu \u00d6zt\u00fcrk","year":"2022","journal-title":"Proteins"},{"key":"2024101506321136700_ref7","doi-asserted-by":"crossref","first-page":"5300","DOI":"10.1021\/acs.biochem.7b00756","article-title":"The phe-ile zipper: a specific interaction motif drives antiparallel coiled-coil hexamer formation","volume":"56","author":"Spencer","year":"2017","journal-title":"Biochemistry"},{"key":"2024101506321136700_ref8","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1471-2148-5-66","article-title":"Coiled-coil protein composition of 22 proteomes\u2014differences and common themes in subcellular infrastructure and traffic control","volume":"5","author":"Rose","year":"2005","journal-title":"BMC Evol Biol"},{"key":"2024101506321136700_ref9","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1007\/s43032-021-00595-2","article-title":"Coiled-coil 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