{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:15:45Z","timestamp":1792793745750,"version":"4.5.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T00:00:00Z","timestamp":1722816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272622"],"award-info":[{"award-number":["82272622"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanxi Fundamental Research Program","award":["20210302123320"],"award-info":[{"award-number":["20210302123320"]}]},{"name":"Non-profit Central Research Institute Fund of National Research Institute For Family Planning","award":["2023GJZD01"],"award-info":[{"award-number":["2023GJZD01"]}],"id":[{"id":"https:\/\/ror.org\/01wnfae18","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M702052"],"award-info":[{"award-number":["2021M702052"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanxi Province Higher Education \u201cBillion Project\u201d Science and Technology Guidance Project","award":["BYJL046"],"award-info":[{"award-number":["BYJL046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Interleukin-32 is a species-specific cytokine that plays an important role in inflammation, cancer, and other diseases; however, its role in reproductive and pregnancy-related diseases remains unknown. This study aimed to investigate the role of interleukin-32 in reproductive and pregnancy-related diseases. Placental tissues from patients with pregnancy-induced hypertension, healthy pregnant women, and trophoblast lines were analysed. Interleukin-32 expression was quantified via polymerase chain reaction and immunohistochemistry, and functional assays were performed after interleukin-32 modulation. Interleukin-32 was identified only in placental mammals, such as Carnivora, Cetartiodactyla, Chiroptera, Dermoptera, Lagomorpha, Perissodactyla, and Primates via bioinformatics. Immunohistochemistry and polymerase chain reaction revealed that interleukin-32 was highly expressed in human placental villi, poorly expressed in decidua and endometrial tissues, and was not detected in mouse tissues. Second, interleukin-32 upregulates miR-205 expression by increasing DROSHA expression, and miR-205 promotes interleukin-32 expression by targeting its promoter region. Interleukin-32 and miR-205 significantly enhanced the invasion ability of HTR8\/SVneo cells (a trophoblast cell line) and the tube formation ability of human umbilical vein endothelial cells. Through quantitative reverse transcription polymerase chain reaction and western blotting, the interleukin-32\/miR-205 loop increased MMP2 and MMP9 expression in HTR-8\/SVneo cells via the nuclear factor kappa B signaling pathway. Finally, using quantitative reverse transcription polymerase chain reaction, interleukin-32 and miR-205 expression levels were significantly lower in the placentas of patients with pregnancy-induced hypertension than in women with normal pregnancies. In conclusion, interleukin-32 regulates trophoblast invasion through the miR-205-nuclear factor kappa B-MMP2\/9 pathway, which is involved in pregnancy-induced hypertension.<\/jats:p>","DOI":"10.1093\/biolre\/ioae118","type":"journal-article","created":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T20:08:31Z","timestamp":1722542911000},"page":"780-799","source":"Crossref","is-referenced-by-count":8,"title":["IL-32 regulates trophoblast invasion through\n                    <i>miR-205<\/i>\n                    -\n                    <i>NF\u03baB<\/i>\n                    -\n                    <i>MMP2\/9<\/i>\n                    axis contributing to the pregnancy-induced hypertension"],"prefix":"10.1093","volume":"111","author":[{"given":"Jianbing","family":"Liu","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]},{"name":"Key Laboratory of Cellular Physiology(Shanxi Medical University), Ministry of Education , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjuan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xihua","family":"Chen","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory, National Research Institute for Family Planning , Da Hui Si Road 12#, Haidian District, Beijing, 100081,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Xinxiang Medical University , Jinhui Road 191#, Xinxiang, 453003, Henan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Shanxi Medical University , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]},{"name":"Key Laboratory of Cellular Physiology(Shanxi Medical University), Ministry of Education , Xinjian South Road 56#, Taiyuan, 030001, Shanxi,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangbo","family":"Xu","sequence":"additional","affiliation":[{"name":"Reproductive Physiology Laboratory, National Research Institute for Family Planning , Da Hui Si Road 12#, Haidian District, Beijing, 100081,","place":["China"]},{"name":"NHC Key Laboratory of Reproductive Health Engineering Technology Research (NRIFP), Da Hui Si Road 12#, Haidian District, Beijing, 100081 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,5]]},"reference":[{"key":"2025080503215999100_ref1","first-page":"131","article-title":"Interleukin-32: a cytokine and inducer of 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