{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T17:38:10Z","timestamp":1780076290723,"version":"3.54.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T00:00:00Z","timestamp":1723075200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071659 to M.Z."],"award-info":[{"award-number":["82071659 to M.Z."]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Basic and Clinical Medical Research Joint Fund of Zhongnan Hospital"},{"DOI":"10.13039\/501100007046","name":"Wuhan University","doi-asserted-by":"publisher","award":["ZNLH202206 to M.Z."],"award-info":[{"award-number":["ZNLH202206 to M.Z."]}],"id":[{"id":"10.13039\/501100007046","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Zhongnan Hospital of Wuhan University Medical Science and Technology Innovation Platform Project","award":["PTXM2020034 to M.Z."],"award-info":[{"award-number":["PTXM2020034 to M.Z."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Impaired extravillous trophoblast (EVT) invasion and resulted poor placentation play a vital role in the development of preeclampsia (PE). However, the underlying mechanisms of dysregulated EVTs remain unclear. This study aimed to explore the role of poly (C)-binding protein 2 (PCBP2), a multifunctional RNA-binding protein, in the pathogenesis of PE and to investigate the detailed signaling pathway. Using qRT-PCR, western blot, and immunohistochemistry, we confirmed that the expression of PCBP2 significantly decreased in placentas from 18 early-onset PE and 30 late-onset PE in comparison to those from 30 normotensive pregnancies. Besides, more significant suppression of PCBP2 was observed in the early-onset type. After transfection of HTR-8\/SVneo with small-interfering RNA specific to PCBP2, the cellular biological behaviors including vitality, immigration, invasiveness, and apoptosis were evaluated by CCK-8 assay, wound-healing assay, transwell assay, and flow cytometry respectively. RNA-seq was applied to screen differentially expressed genes in HTR-8\/SVneo upon PCBP2 silencing. GO and KEGG analysis indicated that WNT signaling pathway and the related processes such as extracellular matrix remodeling and cell adhesion were among the most enriched pathways or processes. Meanwhile, the alternative splicing of WNT5A regulated by PCBP2 was also identified by RIP-seq. Based on HTR-8\/SVneo and villous explant, the regulatory roles of PCBP2 on trophoblast were confirmed to be mediated by WNT5A. Besides, it revealed that ROR2\/JNK\/MMP2\/9 pathway was a vital pathway downstream WNT5A in trophoblast cells. In conclusion, this study suggests that down-regulated PCBP2 impaired the functions of EVTs via suppression of WNT5A-mediating ROR2\/JNK\/MMPs pathway, which may eventually contribute to the development of PE.<\/jats:p>","DOI":"10.1093\/biolre\/ioae122","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T19:54:45Z","timestamp":1723146885000},"page":"1142-1155","source":"Crossref","is-referenced-by-count":3,"title":["Down-regulation of PCBP2 suppresses the invasion and migration of trophoblasts via the WNT5A\/ROR2 pathway in preeclampsia"],"prefix":"10.1093","volume":"111","author":[{"given":"Zhenlie","family":"Chen","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Shenshan Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University , No. 1, Henger Rd. Shanwei, 516621 ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhong","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guigui","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Hubei Clinical Research Center for Prenatal Diagnosis and Birth Health , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan Clinical Research Center for Reproductive Science and Birth Health , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Hubei Provincial Key Laboratory of Developmentally Originated Disease , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Wuhan University , Zhongnan Hospital, , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. China"]},{"name":"Hubei Clinical Research Center for Prenatal Diagnosis and Birth Health , No. 169, East Lake Rd., Wuhan 430071, Hubei Province ,","place":["P. R. 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