{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T16:20:18Z","timestamp":1780330818289,"version":"3.54.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"USDA National Institute of Food and Agriculture\/Hatch","award":["1024792"],"award-info":[{"award-number":["1024792"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD083311"],"award-info":[{"award-number":["HD083311"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mammalian preimplantation development culminates in the formation of a blastocyst that undergoes extensive gene expression regulation to successfully implant into the maternal endometrium. Zinc-finger HIT domain-containing (ZNHIT) 1 and 2 are members of a highly conserved family, yet they have been identified as subunits of distinct complexes. Here, we report that knockout of either Znhit1 or Znhit2 results in embryonic lethality during peri-implantation stages. Znhit1 and Znhit2 mutant embryos have overlapping phenotypes, including reduced proportion of SOX2-positive inner cell mass cells, a lack of Fgf4 expression, and aberrant expression of NANOG and SOX17. Furthermore, we find that the similar phenotypes are caused by distinct mechanisms. Specifically, embryos lacking ZNHIT1 likely fail to incorporate sufficient H2A.Z at the promoter region of Fgf4 and other genes involved in cell projection organization resulting in impaired invasion of trophoblast cells during implantation. In contrast, Znhit2 mutant embryos display a complete lack of nuclear EFTUD2, a key component of U5 spliceosome, indicating a global splicing deficiency. Our findings unveil the indispensable yet distinct roles of ZNHIT1 and ZNHIT2 in early mammalian embryonic development.<\/jats:p>","DOI":"10.1093\/biolre\/ioae128","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T14:34:45Z","timestamp":1724855685000},"page":"1017-1029","source":"Crossref","is-referenced-by-count":3,"title":["Overlapping peri-implantation phenotypes of ZNHIT1 and ZNHIT2 despite distinct functions during early mouse development"],"prefix":"10.1093","volume":"111","author":[{"given":"Xinjian Doris","family":"He","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis F","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosu","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchao","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pharmaceutical Biotechnology , Department of Cardiology, , Nanjing,","place":["China"]},{"name":"Nanjing Drum Tower Hospital, The Affiliated Hospital of Medical School of Nanjing University , Department of Cardiology, , Nanjing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering , School of Life Sciences, , Shanghai,","place":["China"]},{"name":"Zhongshan Hospital, Fudan University , School of Life Sciences, , Shanghai,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongzhou","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pharmaceutical Biotechnology , Department of Cardiology, , Nanjing,","place":["China"]},{"name":"Nanjing Drum Tower Hospital, The Affiliated Hospital of Medical School of Nanjing University , Department of Cardiology, , Nanjing,","place":["China"]},{"name":"MOE Key Laboratory of Model Animal for Disease Study , Model Animal Research Center, , Nanjing,","place":["China"]},{"name":"Medical School of Nanjing University , Model Animal Research Center, , Nanjing,","place":["China"]},{"name":"Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University , Nanjing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Stem Cell and Regenerative Biology , College of Animal Science and Veterinary Medicine, , Wuhan,","place":["China"]},{"name":"Huazhong Agricultural University , College of Animal Science and Veterinary Medicine, , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Liang","family":"Miao","sequence":"additional","affiliation":[{"name":"Institute of Stem Cell and Regenerative Biology , College of Animal Science and Veterinary Medicine, , Wuhan,","place":["China"]},{"name":"Huazhong Agricultural University , College of Animal Science and Veterinary Medicine, , Wuhan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Alfandari","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly D","family":"Tremblay","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Mager","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,28]]},"reference":[{"key":"2024111511492911500_ref1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1002\/dvg.22368","article-title":"Anatomy of a blastocyst: cell behaviors driving cell fate choice and morphogenesis in the early mouse 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