{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T07:32:48Z","timestamp":1769585568706,"version":"3.49.0"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T00:00:00Z","timestamp":1729123200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100020825","name":"Center of Excellence for the Oceans, National Taiwan Ocean University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC 111-2923-B-019-001-MY2"],"award-info":[{"award-number":["NSTC 111-2923-B-019-001-MY2"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Since females grow faster in penaeid shrimp, all-female aquaculture was proposed. Environmental conditions in the Pacific white shrimp were not found to affect genetic sex determination (ZZ\/ZW system). The androgenic gland\u2013secreting insulin-like androgenic gland hormone is a key controlling factor in crustacean male differentiation. However, functional sex reversal (neo-male) in penaeid shrimp has not yet been achieved by manipulating the insulin-like androgenic gland hormone\u2013sexual switch. Therefore, understanding the molecular mechanisms of gonadal differentiation may help build appropriate tools to generate neo-male for all-female breeding. This study describes the potential role of the novel penaeid-specific testicular zinc finger protein (pTZFP) in the gonads of Pacific white shrimp. First, pTZFP transcripts show a male-bias expression pattern in undifferentiated gonads, which is then exclusively expressed in the testis and absent or slightly expressed in the ovary and other tissues. Besides, the knockdown of pTZFP in undifferentiated males results in smaller testes but no sex reversal. Immunohistochemical staining of proliferating cell nuclear antigen further confirmed that the smaller testes in pTZFP-deficient males are due to the lower proliferating activity of spermatogonia. These data reveal that pTZFP may be involved in testicular development but have fewer effects on gonadal differentiation. Moreover, testicular pTZFP transcription levels were not reduced with estradiol-17\u03b2 (E2) administration or AG excision. Therefore, our data suggest that pTZFP may regulate testicular development through downstream genes regulating spermatogonia proliferation. Moreover, our data provide an appropriate molecular marker for identifying the sex of undifferentiated gonads.<\/jats:p>","DOI":"10.1093\/biolre\/ioae151","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T18:27:31Z","timestamp":1729189651000},"page":"179-190","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of a novel and testis-specific zinc finger protein during sexual development of Pacific white shrimp <i>Litopenaeus vannamei<\/i>"],"prefix":"10.1093","volume":"112","author":[{"given":"Chi-Sheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao-Sheng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan-Ting","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Chieh","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng-Wei","family":"Tseng","sequence":"additional","affiliation":[{"name":"Doctoral Degree Program in Marine Biotechnology, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]},{"name":"Doctoral Degree Program in Marine Biotechnology, Academia Sinica , Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hau-Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Sagi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Ben-Gurion University of the Negev , Beer-Sheva ,","place":["Israel"]},{"name":"National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev , Beer-Sheva ,","place":["Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Fong","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]},{"name":"Center of Excellence for the Oceans, National Taiwan Ocean University , 2 Pei-NIng Road, Zhongzheng District, Keelung City 202 ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan-Chung","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, National Taiwan Ocean University , Keelung ,","place":["Taiwan"]},{"name":"Center of Excellence for the Oceans, National Taiwan Ocean University , 2 Pei-NIng Road, Zhongzheng District, Keelung City 202 ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"key":"2025011610390202100_ref1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.3389\/fendo.2020.00651","article-title":"The \"IAG-switch\"-a key controlling element in decapod crustacean sex differentiation","volume":"11","author":"Levy","year":"2020","journal-title":"Front Endocrinol"},{"key":"2025011610390202100_ref2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/0044-8486(94)90291-7","article-title":"Growth and nutrient digestibility by male and female Penaeus monodon: evidence of sexual 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