{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T10:39:45Z","timestamp":1782643185487,"version":"3.54.5"},"reference-count":128,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"National Institute of Environmental Health Sciences of the National Institutes of Health","award":["T32ES007062"],"award-info":[{"award-number":["T32ES007062"]}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01 ES030374"],"award-info":[{"award-number":["R01 ES030374"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Developmental exposure to environmental chemicals perturbs establishment and maintenance of the ovarian reserve across the reproductive lifetime, leading to premature follicle depletion and ovarian aging. Considering humans are exposed to a complex mixture of environmental chemicals, real-life models assessing their cumulative impact on the ovarian reserve are needed. Biosolids are a source of a real-life mixture of environmental chemicals. While earlier studies demonstrated that grazing pregnant sheep on biosolids-treated pastures did not influence establishment of the ovarian reserve in fetal life, its impact on subsequent depletion of ovarian reserve during reproductive life of offspring is unknown. We hypothesized that developmental exposure to biosolids accelerates depletion of ovarian reserve. Ovaries were collected from F1 juveniles (9.5\u00a0weeks) and adults (2.5\u00a0years) born to F0 ewes grazed on control inorganic fertilizer pastures or biosolids-treated pastures from before conception and throughout gestation. The impact on follicular density, activation rate, and anti-M\u00fcllerian hormone (mediator of activation) expression by immunohistochemistry was determined. Activation rate was increased in F1 biosolids-treated pastures juveniles with a corresponding reduction in primordial follicle density. In contrast, activation rate and ovarian reserve were similar between control and F1 biosolids-treated pastures adults. The density of anti-M\u00fcllerian hormone-positive antral follicles was lower in biosolids-treated pastures juveniles, whereas anti-M\u00fcllerian hormone expression tended to be higher in antral follicles of biosolids-treated pastures adults, consistent with the changes in the ovarian reserve. These findings of detrimental effects of developmental exposure to biosolids during juvenile life that normalizes in adults is supportive of a shift in activation rate likely related to peripubertal hormonal changes.<\/jats:p>","DOI":"10.1093\/biolre\/ioae166","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T18:13:07Z","timestamp":1732039987000},"page":"331-345","source":"Crossref","is-referenced-by-count":4,"title":["Developmental programming: preconceptional and gestational exposure of sheep to biosolids on offspring ovarian dynamics"],"prefix":"10.1093","volume":"112","author":[{"given":"Katherine M","family":"Halloran","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Michigan , Ann Arbor, MI ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiran","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan , Ann Arbor, MI ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Bellingham","sequence":"additional","affiliation":[{"name":"School of Biodiversity One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G","family":"Lea","sequence":"additional","affiliation":[{"name":"Schools of Biomedicines and Veterinary Medicine and Science, University of Nottingham , Sutton Bonington Campus, Loughborough ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil P","family":"Evans","sequence":"additional","affiliation":[{"name":"School of Biodiversity One Health and Veterinary Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin D","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Schools of Biomedicines and Veterinary Medicine and Science, University of Nottingham , Sutton Bonington Campus, Loughborough ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Smith","sequence":"additional","affiliation":[{"name":"Agricultural Systems and Reproduction, Animal Science , Invermay Agricultural Centre, AgResearch Ltd, Puddle Alley, Mosgiel ,","place":["New Zealand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasantha","family":"Padmanabhan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Michigan , Ann Arbor, MI 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