{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:01:12Z","timestamp":1780639272604,"version":"3.54.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Key Projects of Natural Science Research in Colleges and Universities in Anhui Province","award":["2023AH053178"],"award-info":[{"award-number":["2023AH053178"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81801511"],"award-info":[{"award-number":["81801511"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Intrauterine adhesions (IUA) represent a prevalent uterine endometrial disorder frequently correlated with menstrual irregularities and infertility. Some members of the secretoglobin(SCGB) family have demonstrated anti-fibrotic effects, however, the specific role of SCGB1D4, one of the family members, in anti-fibrosis remains unclear. This study aimed to investigate the expression of SCGB1D4 in IUA tissues, validate the role of SCGB1D4 in endometrial fibrosis, and assess its potential therapeutic significance by analyzing clinical features and constructing rat and cell models. Clinical characteristics of patients with intrauterine adhesions (IUA) were compared and analyzed against control subjects. Additionally, a rat uterine adhesion model was successfully established using a combination of mechanical injury and infection. The expression levels of SCGB1D4 in patient tissues and animal models were detected through immunohistochemistry, Western blot, and real-time fluorescence quantitative PCR, and the changes in fibrosis markers COL1A1 and \u03b1-SMA were also evaluated. Furthermore, human endometrial stromal cell lines (HESCs) induced by transforming growth factor-\u03b2-1 conversion were differentiated into myofibroblasts to establish cell models of intrauterine adhesion. We detected the expression of SCGB1D4 and fibrosis-related factors by real-time fluorescence quantitative PCR and Western blot. Cell proliferation and cell cycle changes were assessed using flow cytometry and CCK8. IUA patients showed increased miscarriage rates and decreased endometrial thickness. Clinical tissue specimens revealed significantly lower expression of SCGB1D4 in the endometrial tissues of IUA patients, accompanied by a notable increase in COL1A1 and \u03b1-SMA. The established rat model of intrauterine adhesion exhibited decreased expression of SCGB1D4 and a significant increase in fibrosis. After overexpression of SCGB1D4 on the IUA cell model, SCGB1D4 expression was elevated, while COL1A1 and \u03b1-SMA expression was significantly reduced. Cell proliferation was inhibited and cell cycle distribution was altered. This study has confirmed the low expression of SCGB1D4 in patients with IUA, as well as in animal and cell models. Furthermore, the overexpression of SCGB1D4 in a cell model of IUA demonstrates that it may play a key role in inhibiting fibrosis. SCGB1D4 holds promise as a potential therapeutic target for IUA, providing a new avenue for overcoming fertility issues caused by IUA.<\/jats:p>","DOI":"10.1093\/biolre\/ioae172","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T12:38:14Z","timestamp":1732624694000},"page":"273-285","source":"Crossref","is-referenced-by-count":1,"title":["SCGB1D4 downregulation links to fibrosis in intrauterine adhesion patients and rat models"],"prefix":"10.1093","volume":"112","author":[{"given":"Jing","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanchan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunmeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiying","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Anhui Medical University , Hefei, Anhui ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"2025021811360769800_ref1","doi-asserted-by":"crossref","first-page":"5890","DOI":"10.1038\/s41467-023-41656-1","article-title":"Decoding the endometrial niche of Asherman's syndrome at single-cell resolution","volume":"14","author":"Santamaria","year":"2023","journal-title":"Nat Commun"},{"key":"2025021811360769800_ref2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12958-022-00887-5","article-title":"EndMT: new findings on the origin of myofibroblasts in endometrial fibrosis of intrauterine adhesions","volume":"20","author":"Xu","year":"2022","journal-title":"Reprod Biol Endocrinol"},{"key":"2025021811360769800_ref3","article-title":"LAMB3 promotes Myofibrogenesis and cytoskeletal reorganization in endometrial stromal cells via the RhoA\/ROCK1\/MYL9 pathway","volume":"82","author":"Qin","year":"2023","journal-title":"Cell Biochem Biophys"},{"key":"2025021811360769800_ref4","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3390\/jcm12041319","article-title":"Platelet-rich plasma improves pregnancy outcomes in moderate to severe intrauterine adhesion: a retrospective cohort study","volume":"12","author":"Qiu","year":"2023","journal-title":"J Clin Med"},{"key":"2025021811360769800_ref5","volume-title":"StatPearls","author":"Smikle","year":"2023"},{"key":"2025021811360769800_ref6","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1186\/1479-7364-5-6-691","article-title":"Update of the human secretoglobin (SCGB) gene superfamily and an example of 'evolutionary bloom' of androgen-binding protein genes within the mouse Scgb gene superfamily","volume":"5","author":"Jackson","year":"2011","journal-title":"Hum Genomics"},{"key":"2025021811360769800_ref7","first-page":"201","article-title":"Significant association between SCGB1D4 gene polymorphisms and susceptibility to adenoid hypertrophy in a pediatric population. 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