{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T10:35:59Z","timestamp":1783074959683,"version":"3.54.6"},"reference-count":72,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000071","name":"NICHD","doi-asserted-by":"publisher","award":["R21HD105963"],"award-info":[{"award-number":["R21HD105963"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01HD110170"],"award-info":[{"award-number":["R01HD110170"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The adult mammalian testis is filled with seminiferous tubules, which contain somatic Sertoli cells along with germ cells undergoing all phases of spermatogenesis. During spermatogenesis in postnatal mice, male germ cells undergo at least 17 different nomenclature changes as they proceed through mitosis as spermatogonia (=8), meiosis as spermatocytes (=6), and spermiogenesis as spermatids (=3). Adding to this complexity, combinations of germ cells at each of these stages of development are clumped together along the length of the seminiferous tubules. Due to this, considerable expertise is required for investigators to accurately analyze changes in spermatogenesis in animals that have spontaneous mutations, have been genetically modified (transgenic or knockout\/knockin), or have been treated with pharmacologic agents. Here, we leverage our laboratory\u2019s expertise in spermatogenesis to optimize the open-source \u201cQuantitative Pathology &amp; Bioimage Analysis\u201d software platform for automated analyses of germ and somatic cell populations in both the developing and adult mammalian testis.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf011","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T12:49:07Z","timestamp":1737031747000},"page":"583-599","source":"Crossref","is-referenced-by-count":1,"title":["Utilization of the QuPath open-source software platform for analysis of mammalian spermatogenesis"],"prefix":"10.1093","volume":"112","author":[{"given":"Bryan A","family":"Niedenberger","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University , Greenville, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather A","family":"Belcher","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University , Greenville, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma A","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University , Greenville, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University , Greenville, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher B","family":"Geyer","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University , Greenville, NC ,","place":["USA"]},{"name":"East Carolina Diabetes and Obesity Institute, East Carolina University , Greenville, NC ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"2025031708041697700_ref1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/ar.1091840207","article-title":"Three-dimensional organization of testicular interstitial tissue and lymphatic space in the rat","volume":"184","author":"Clark","year":"1976","journal-title":"Anat Rec"},{"key":"2025031708041697700_ref2","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2164\/jandrol.109.008235","article-title":"Illuminating testis morphogenesis in the mouse","volume":"31","author":"Nel-Themaat","year":"2010","journal-title":"J Androl"},{"key":"2025031708041697700_ref3","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1095\/biolreprod.113.111682","article-title":"Novel stage classification of human 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