{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:21:42Z","timestamp":1781760102249,"version":"3.54.5"},"reference-count":79,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T00:00:00Z","timestamp":1740268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Department of Agriculture, National Institute of Food and Agriculture, and Agriculture and Food Research Initiative (USDA NIFA AFRI) Competitive","award":["2019-67015-28998"],"award-info":[{"award-number":["2019-67015-28998"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A central determinant of successful reproduction is pregnancy establishment and maintenance that relies on proper development of the conceptus (embryo\/fetus and associated extraembryonic membranes including the placenta). Pregnancy loss in cattle can be caused by inadequate development and differentiation of the placenta. However, the cellular and molecular mechanisms regulating bovine placenta development and, particularly, trophoblast differentiation are not well understood. Recent single-cell RNA-seq analyses revealed dynamic changes in cell populations and gene expression patterns during bovine placental development. Here, the chromatin accessibility landscape across diverse cell populations was determined in the developing (Day 40) and mature (Day 170) bovine placenta using the 10X Genomics multiome (snRNA-seq and snATAC-seq) platform. Analyses revealed distinct trophoblast, mesenchyme, endothelial, immune, and epithelial cell populations characterized by unique gene expression and chromatin accessibility signatures. ATAC-seq peaks defined open chromatin regions, facilitating the identification of transcription factor binding sites and candidate gene regulatory networks involved with trophoblast differentiation. Several transcription factors, known for their involvement in trophoblast differentiation in other mammalian species, were identified as candidate regulators of uninucleate to binucleate trophoblast differentiation. This study adds to our foundational understanding of gene regulation and expression in the placenta, offering insights into the mechanisms governing pregnancy loss in cattle.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf036","type":"journal-article","created":{"date-parts":[[2025,2,23]],"date-time":"2025-02-23T16:39:06Z","timestamp":1740328746000},"page":"955-968","source":"Crossref","is-referenced-by-count":3,"title":["Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation"],"prefix":"10.1093","volume":"112","author":[{"given":"Kimberly M","family":"Davenport","sequence":"first","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO ,","place":["United States"]},{"name":"Department of Animal Sciences, Washington State University , Pullman, WA ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makenzie T","family":"Lowke","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Sofia","family":"Ortega","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison , Madison, WI ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M","family":"Kelleher","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health, University of Missouri , Columbia, MO ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley C","family":"Warren","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO ,","place":["United States"]},{"name":"Department of Surgery, School of Medicine, University of Missouri , Columbia, MO ,","place":["United States"]},{"name":"Institute for Data Science and Informatics, University of Missouri , Columbia, MO ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E","family":"Spencer","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO ,","place":["United States"]},{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health, University of Missouri , Columbia, MO ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,2,23]]},"reference":[{"key":"2025052711582868600_ref1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0303-7207(94)90285-2","article-title":"Regulation of trophoblast interferon gene expression","volume":"100","author":"Flint","year":"1994","journal-title":"Mol Cell 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