{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T13:48:30Z","timestamp":1768744110379,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T00:00:00Z","timestamp":1741478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Peak academic talents of Jiangsu Provincial Hospital of Traditional Chinese Medicine","award":["y2021rc11"],"award-info":[{"award-number":["y2021rc11"]}]},{"name":"Major Social Development Projects in Jiangsu Province","award":["BE2021726"],"award-info":[{"award-number":["BE2021726"]}]},{"name":"Scientific Research Project of Health Commission of Jiangsu Province","award":["M2020089"],"award-info":[{"award-number":["M2020089"]}]},{"name":"National Natural Science Foundation of China Youth Fund","award":["82104903"],"award-info":[{"award-number":["82104903"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Purpose<\/jats:title>\n                  <jats:p>To investigate how steroid receptor RNA activator (SRA) regulates follicular development in mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Systemic SRA knockout mice were introduced. SRA expression was reinstated in the anteroventral periventricular nucleus (AVPV) of the hypothalamus using lentiviral vectors. Subsequently, the estrous cycle, serum hormone levels, follicle development, and hypothalamic kisspeptin expression in mice were assessed. Kiss1 promoter activity was tested with a fluorescent reporter system in Neuro-2a cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>SRA deficiency caused a shift to shorter metestrus and longer diestrus phases, reduced numbers of large antral and preovulatory follicles, increased formation of atretic cyst-like follicles, lowered serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol (E2), and decreased expression of hypothalamic AVPV-kisspeptin in mice. The reinstatement of SRA expression in the AVPV nucleus normalized kisspeptin expression, hormone levels, and follicle development. In Neuro-2a cells, SRA increased Kiss1 transcription upon E2 treatment, a response that was nullified by the estrogen receptor alpha (ER\u03b1) inhibitor.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>SRA enhances ER\u03b1-mediated Kiss1 transcription in the AVPV nucleus to control the kisspeptin-GPR54 system in the hypothalamus, essential for regulating ovulation through the hypothalamus-pituitary-ovary axis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/biolre\/ioaf049","type":"journal-article","created":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T20:24:56Z","timestamp":1741551896000},"page":"1114-1122","source":"Crossref","is-referenced-by-count":1,"title":["SRA deficiency induces follicular dysplasia by disrupting the hypothalamic Kisspeptin-GPR54 system in mice"],"prefix":"10.1093","volume":"112","author":[{"given":"Jing","family":"Jin","sequence":"first","affiliation":[{"name":"Department of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhui","family":"Kou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine , Shenzhen, Guangdong ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Nanjing Lishui District Maternity and Child Health Care Hospital , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinyin","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keshu","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongning","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Nanjing Lishui District Maternity and Child Health Care Hospital , Nanjing, Jiangsu ,","place":["China"]},{"name":"Department of Gynecology, Women\u2019s Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huifang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Affiliated Hospital of Nanjing University of Chinese Medicine , Nanjing, Jiangsu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,3,9]]},"reference":[{"key":"2025062507101806800_ref1","first-page":"7","article-title":"Hypothalamic-pituitary-ovarian Axis disorders impacting female fertility","volume":"7","author":"Mikhael","year":"2019","journal-title":"Biomedicine"},{"key":"2025062507101806800_ref2","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.fertnstert.2020.06.038","article-title":"Role of kisspeptins in the control of the hypothalamic-pituitary-ovarian axis: old dogmas and new 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