{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T20:07:08Z","timestamp":1766088428932,"version":"3.41.0"},"reference-count":99,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T00:00:00Z","timestamp":1741824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CRISP2 is enriched in the male reproductive system of mammals and plays roles in spermatogenesis, sperm motility, and fertilization. Although extensively investigated in rodents and boars, human CRISP2 (hCRISP2) remains poorly studied, particularly concerning its localization in testicular and epididymal tissues and its molecular features. In this study, we used immunofluorescence to determine the localization of hCRISP2 in testis, epididymis, and ejaculated sperm. While no expression was observed in the epididymal epithelium, hCRISP2 was detected at different stages during spermatogenesis. Specifically, hCRISP2 was found in the nucleus of primary spermatocytes and of both round and early elongated spermatids. In elongated spermatids, it was additionally observed in the cytoplasm, the flagellum, and the equatorial segment of the acrosome (EqS). The presence of aggregated material with hCRISP2 immunoreactivity in the apical pole of Sertoli cells suggests that most of the hCRISP2 involved in spermatogenesis is phagocytized by these cells during spermiation. In ejaculated sperm, hCRISP2 was found in the cytoplasmic droplet, flagellum, and EqS, consistent with its described roles in sperm motility and gamete fusion. Native and denaturing electrophoresis combined with western blot analyses depicted the ability of hCRISP2 to form stable high molecular weight complexes, and mass spectrometry revealed that these complexes likely consist exclusively of hCRISP2. Furthermore, we showed that hCRISP2 undergoes only limited post-translational modifications. These findings shed light into the dynamic localization of hCRISP2 throughout spermatogenesis and in ejaculated sperm, as well as its molecular features, enhancing our understanding of its functional roles and relevance for male fertility.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf051","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T08:58:59Z","timestamp":1741856339000},"page":"1167-1184","source":"Crossref","is-referenced-by-count":1,"title":["Novel insights into human CRISP2: localization in reproductive tissues and sperm, and molecular characterization"],"prefix":"10.1093","volume":"112","author":[{"given":"Thibault","family":"Masai","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandine","family":"Delnatte","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Dendievel","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Nonclercq","sequence":"additional","affiliation":[{"name":"Laboratory of Histology, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annica","family":"Frau","sequence":"additional","affiliation":[{"name":"Laboratory of Histology, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Fran\u00e7ois","family":"Simon","sequence":"additional","affiliation":[{"name":"Clinique de Fertilit\u00e9 R\u00e9gionale de Mons, Centres Hospitaliers Universitaires (CHU) HELORA\u2014H\u00f4pital de Mons site Kennedy , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Arcolia","sequence":"additional","affiliation":[{"name":"Clinique de Fertilit\u00e9 R\u00e9gionale de Mons, Centres Hospitaliers Universitaires (CHU) HELORA\u2014H\u00f4pital de Mons site Kennedy , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruddy","family":"Wattiez","sequence":"additional","affiliation":[{"name":"Laboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baptiste","family":"Leroy","sequence":"additional","affiliation":[{"name":"Laboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia S","family":"Cuasnicu","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental - Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (IByME-CONICET) , Ciudad Aut\u00f3noma de Buenos Aires ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Lybaert","sequence":"additional","affiliation":[{"name":"Research Laboratory on Human Reproduction, Faculty of Medicine, Universit\u00e9 libre de Bruxelles , Brussels ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elise","family":"Hennebert","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Research Institute for Biosciences, Research Institute for Health Sciences and Technology, University of Mons , Mons ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,3,13]]},"reference":[{"key":"2025062507162221600_ref1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1007\/BF00292155","article-title":"The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes","volume":"3","author":"Mizuki","year":"1992","journal-title":"Mamm Genome"},{"key":"2025062507162221600_ref2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/j.1432-1033.1996.t01-1-00827.x","article-title":"The human cysteine-rich secretory protein (CRISP) family. 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