{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:34:31Z","timestamp":1787168071746,"version":"build-2736575974"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T00:00:00Z","timestamp":1747094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Science and Technology Plan Program of the Guiyang Bureau","award":["[2019]9\u20131-1"],"award-info":[{"award-number":["[2019]9\u20131-1"]}]},{"name":"Science and Technology Fund Project of Guizhou Health Commission","award":["gzwkj2024\u2013439"],"award-info":[{"award-number":["gzwkj2024\u2013439"]}]},{"name":"Science and Technology Plan Project of Qianxinan Prefecture"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Background: Premature ovarian insufficiency (POI) is the loss of ovarian function among women &amp;lt;40\u00a0years of age, and immune disorders play a critical role in POI development. Indoleamine 2,3-dioxygenase (IDO) catalyzes tryptophan metabolism via the kynurenine pathway and plays a key role in preventing and treating immune-related diseases. Methods: Human ovarian granulosa cells (hGCs) were collected via the density gradient method, zona pellucida protein 3 was used to establish an immune POI mouse model, and an adeno-associated virus (AAV) vector carrying IDO1 (IDO-AAV) was injected into mouse ovaries to induce IDO overexpression. Ovarian function was measured by the estrous cycle, serum anti-M\u00fcllerian hormone concentration, degree of ovarian fibrosis, and number of follicles. Findings: IDO protein levels and mRNA expression in hGCs were lower in the POI group than in the control group (P\u2009&amp;lt;\u20090.05). Both the ovarian function and IDO levels in the POI\u2009+\u2009Water group and the POI\u2009+\u2009Glu group were significantly lower than those in the control group. In POI model mice injected with IDO-AAV, ovarian function, and the CD4+ CD25+ Foxp3+ regulatory T (Treg) cell proportion were increased compared with those in mice injected with the natural control AAV. The FoxP3 mRNA expression level in Treg cells was positively correlated with the IDO mRNA expression level, whereas the ROR\u03b3t mRNA expression level in Th17 cells was negatively correlated with the IDO mRNA expression level, further suggesting that IDO may be related to Treg and Th17 cells through AhR and subsequently regulate immunity and ovarian function. Conclusion: Increasing ovarian IDO levels in POI mice improved ovarian function.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf102","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T07:49:32Z","timestamp":1747036172000},"page":"331-344","source":"Crossref","is-referenced-by-count":5,"title":["Indoleamine 2,3-dioxygenase can improve ovarian function in premature ovarian insufficiency via aromatic hydrocarbon receptor and regulatory T cells"],"prefix":"10.1093","volume":"113","author":[{"given":"Anchun","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology, School of Clinical Medicine, Guizhou Medical University , Guiyang, Guizhou ,","place":["China"]},{"name":"Reproductive Medicine Centre, Department of Obstetrics and Gynaecology, Xingyi People\u2019s Hospital, Qianxina Prefecture , Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, School of Clinical Medicine, Guizhou Medical University , Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanyou","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, School of Clinical Medicine, Guizhou Medical University , Guiyang, Guizhou ,","place":["China"]},{"name":"Reproductive Medicine Centre, Affiliated Hospital of Guizhou Medical University , Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Xingyi People\u2019s Hospital, Qianxinan Prefecture , Guizhou 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University , Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, School of Clinical Medicine, Guizhou Medical University , Guiyang, Guizhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,5,13]]},"reference":[{"key":"2025081723472920600_ref1","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1093\/humrep\/dew027","article-title":"ESHRE guideline: management of women with premature ovarian insufficiency","volume":"31","author":"Webber","year":"2016","journal-title":"Hum Reprod"},{"key":"2025081723472920600_ref2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1080\/13697137.2019.1574738","article-title":"The global prevalence of primary ovarian insufficiency and early menopause: a 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