{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:31:30Z","timestamp":1788492690904,"version":"build-2803163510"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The quality of mouse oocytes that are matured in vitro is much lower than the ones that are matured in vivo. This difference is mainly due to oxidative stress occurring in culture conditions. Astaxanthin, a marine carotenoid, is one of the most robust natural antioxidants that can effectively reduce oxidative stress. However, the role of astaxanthin in oocyte in vitro maturation (IVM) has not been reported. Here, we showed that the addition of astaxanthin successfully increased the maturation rate of mouse IVM oocytes. Single-cell transcriptome sequencing showed that the differential genes were mainly enriched to mitochondrial functions and protein homeostasis. By using micro-proteomics, we further showed that the mitochondrial activity was enhanced by astaxanthin addition. To reveal the developmental competence of astaxanthin-treated IVM oocytes and conquer the zona pellucida hardening, we applied zona pellucida laser optoperforation to assist the in vitro fertilization. We found that the 2-cell embryo rate was significantly increased in astaxanthin-treated oocytes. In summary, this study reveals that astaxanthin can efficiently promote mouse oocyte IVM and early embryonic development.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf107","type":"journal-article","created":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T08:06:10Z","timestamp":1746259570000},"page":"345-357","source":"Crossref","is-referenced-by-count":5,"title":["Astaxanthin promotes in vitro maturation of mouse oocytes by regulating mitochondrial functions and protein homeostasis"],"prefix":"10.1093","volume":"113","author":[{"given":"Xinyi","family":"Yuan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University , Jinan ,","place":["China"]},{"name":"National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University , Jinan ,","place":["China"]},{"name":"Key Laboratory of Reproductive 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Shandong University , Jinan ,","place":["China"]},{"name":"Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education , Jinan ,","place":["China"]},{"name":"Shandong Technology Innovation Center for Reproductive Health , Jinan ,","place":["China"]},{"name":"Shandong Provincial Clinical Research Center for Reproductive Health , Jinan ,","place":["China"]},{"name":"Shandong Key Laboratory of Reproductive Research and Birth Defect Prevention , Jinan ,","place":["China"]},{"name":"Research Unit of Gametogenesis and Health of ART-Offspring , Chinese Academy of Medical Sciences, Jinan ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University , Jinan ,","place":["China"]},{"name":"National 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