{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:59:17Z","timestamp":1772884757178,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Outstanding Talent Research Funding of Xuzhou Medical University"},{"name":"Natural Science Research of the Jiangsu Higher Education Institutions of China"},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Natural Science Foundation Program"},{"name":"National Natural Science Foundation Program"},{"name":"Shandong Provincial University Youth Innovation Team, China"},{"name":"Shandong Province Medical Health Science and Technology Project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Polycystic ovary syndrome (PCOS) lacks the generally accepted diagnostic biomarkers and targeted therapy. Increasing evidence indicates that microRNAs play a crucial role in PCOS. Hereby, we tested the functional implications of a novel MicroRNA (miR-423-3p) as a mediator in the progress of hyperandrogenic PCOS, as well as its potential as a new serum biomarker and therapeutic target for the PCOS. We found significantly decreased miR-423-3p levels in serum, human granulosa cells (hGCs), and follicular fluid of PCOS patients (n\u2009=\u200940) compared to healthy controls (n\u2009=\u200930), and this decrease corroborated in PCOS-like mouse models. The receiver operating characteristic curve analysis for circulating miR-423-3p indicated high diagnostic potential as a biomarker, with an area under the curve of 82%. miR-423-3p influenced hGC (Human ovarian granulosa cell line KGN) proliferation by directly targeting CTNNBIP1-modulated Wingless-type (WNT) signaling pathway. We further proved as mechanistic role that the elevated dihydrotestosterone inhibited the expression of miR-423-3p via the activation of the androgen receptor, and the overexpression of miR-423-3p normalized the function of androgen-induced GCs. While we overexpressed miR-423-3p, it counteracted androgen-induced dysfunction in GCs. Antiandrogen treatment restored the reproductive phenotypes in letrozole-induced PCOS-like mice and regulated miR-423-3p expression and its downstream effects. Ovarian intrabursal injection of miR-423-3p antagomir in wild-type mice induced PCOS-like phenotypes, further underscoring its functional role. Our results demonstrated that miR-423-3p emerged as a novel mediator in hyperandrogenic PCOS progression, and it holds promise as both a diagnostic biomarker and a therapeutic target.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf116","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T08:35:41Z","timestamp":1751445341000},"page":"856-869","source":"Crossref","is-referenced-by-count":2,"title":["miR-423-3p inhibits CTNNBIP1\/WNT preventing hyperandrogenic polycystic ovary syndrome"],"prefix":"10.1093","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3738-4602","authenticated-orcid":false,"given":"Shanshan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences, Jining Medical University , No. 669 Xueyuan Road, Donggang District, Rizhao, Shandong Province 276826 ,","place":["PR China"]},{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University , No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193 ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajing","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Tsinghua University , No. 30 Shuangqing Road, Qinghuayuan Subdistrict, Haidian District, Beijing 100084 ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Basic Medical Sciences, Xuzhou Medical University , No. 209 Tongshan Road, Xuzhou, Jiangsu Province 221009 ,","place":["PR China"]},{"name":"National Experimental Teaching Demonstration Center for Basic Medicine, Xuzhou Medical University , No. 209 Tongshan Road, Xuzhou, Jiangsu Province 221009 ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donata","family":"Ponikwicka-Tyszko","sequence":"additional","affiliation":[{"name":"Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , W\u0142adys\u0142awa Tryli\u0144skiego 18, 10-683 Olsztyn ,","place":["Poland"]},{"name":"Institute of Biomedicine, Faculty of Medicine, University of Turku , Kiinamyllynkatu 10 20520, Turku ,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Slawomir","family":"Wolczynski","sequence":"additional","affiliation":[{"name":"Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences , W\u0142adys\u0142awa Tryli\u0144skiego 18, 10-683 Olsztyn ,","place":["Poland"]},{"name":"Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok , Ul M. Sklodowskiej-Curie 24 A, 15-276 Bialystok ,","place":["Poland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Affiliated Jinling Hospital, Medicine School of Nanjing University , No. 305 Zhongshan East Road, Xuanwu District, Nanjing 210002, Jiangsu ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Affiliated Jinling Hospital, Medicine School of Nanjing University , No. 305 Zhongshan East Road, Xuanwu District, Nanjing 210002, Jiangsu ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4184-9540","authenticated-orcid":false,"given":"Bing","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Affiliated Jinling Hospital, Medicine School of Nanjing University , No. 305 Zhongshan East Road, Xuanwu District, Nanjing 210002, Jiangsu ,","place":["PR China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nafis A","family":"Rahman","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Faculty of Medicine, University of Turku , Kiinamyllynkatu 10 20520, Turku ,","place":["Finland"]},{"name":"Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok , Ul M. Sklodowskiej-Curie 24 A, 15-276 Bialystok ,","place":["Poland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangdong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University , No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193 ,","place":["PR China"]},{"name":"Department of Reproduction and Gynecological Endocrinology, Medical University of Bialystok , Ul M. Sklodowskiej-Curie 24 A, 15-276 Bialystok ,","place":["Poland"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"2025101516135752500_ref1","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/nrendo.2014.102","article-title":"The management of patients with polycystic ovary syndrome","volume":"10","author":"Jayasena","year":"2014","journal-title":"Nat Rev Endocrinol"},{"key":"2025101516135752500_ref2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ijgo.2008.01.023","article-title":"Prevalence of symptoms associated with polycystic ovary syndrome","volume":"102","author":"Lindholm","year":"2008","journal-title":"Int J Gynaecol Obstet"},{"key":"2025101516135752500_ref3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1056\/NEJMoa1903307","article-title":"Apalutamide for metastatic, castration-sensitive prostate 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