{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:41:16Z","timestamp":1793144476505,"version":"4.5.0"},"reference-count":65,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T00:00:00Z","timestamp":1748649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Vanderbilt Mouse Metabolic Phenotyping Center","award":["S10OD025199"],"award-info":[{"award-number":["S10OD025199"]}]},{"name":"Vanderbilt Mouse Metabolic Phenotyping Center","award":["DK135073"],"award-info":[{"award-number":["DK135073"]}]},{"name":"Vanderbilt Mouse Metabolic Phenotyping Center","award":["DK020593"],"award-info":[{"award-number":["DK020593"]}]},{"name":"National Institute of Health","award":["R01HD108166"],"award-info":[{"award-number":["R01HD108166"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Assisted reproductive technologies (ARTs) have rapidly evolved since being introduced in 1978. However, many of the procedures used clinically lack a basis of long-term studies to ensure their safety. Though in vitro fertilization (IVF) is largely safe, follow-up studies have shown that IVF-conceived children may show signs of altered fat deposition, increased fasting glucose, and increased blood pressure. These results are, however, limited by a low number of patients and different ART variables (different lengths of embryo culture or different types of culture media). Results of studies using animal models have confirmed many of these results and shown that more stressful culture conditions result in disrupted adult phenotypes. Presently, it is unclear if culture conditions such as duration and day of transfer might affect adult health. To investigate the hypothesis that the length of embryo culture could affect adult phenotype, we generated mice by IVF and transferred them at the cleavage stage (IVF8C group) or at the blastocyst stage (IVFBL group) and studied adult phenotype. Results were compared to those obtained with naturally conceived animals flushed out of the uterus and transferred to the recipient (FB group). We found sexual dimorphic effects with male mice showing a more severe phenotype. Male offspring resulting from cleavage stage transfer showed altered glucose handling, left cardiac dysfunction, and shorter lifespan, while male offspring post blastocyst transfer showed reduced locomotor activity. Female mice showed a milder phenotype, particular for female offspring generated by transfer at the cleavage stage.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf120","type":"journal-article","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T07:43:34Z","timestamp":1748677414000},"page":"657-671","source":"Crossref","is-referenced-by-count":4,"title":["Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice"],"prefix":"10.1093","volume":"113","author":[{"given":"Rhodel K","family":"Simbulan","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco , San Francisco, CA ,","place":["USA"]},{"name":"Reproductive Clinical Sciences Program, Macon & Joan Brock Virginia Health Sciences at Old Dominion University , Norfolk, VA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco , San Francisco, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza K","family":"Oqani","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco , San Francisco, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco , San Francisco, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Lantier","sequence":"additional","affiliation":[{"name":"Vanderbilt Mouse Metabolic Phenotyping Center , Department of Molecular Physiology & Biophysics, Vanderbilt University 815 Light Hall 2215 Garland Avenue Nashville, TN , 37232","place":["USA"]},{"name":"Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine , Nashville, TN ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Owen P","family":"McGuinness","sequence":"additional","affiliation":[{"name":"Vanderbilt Mouse Metabolic Phenotyping Center , Department of Molecular Physiology & Biophysics, Vanderbilt University 815 Light Hall 2215 Garland Avenue Nashville, TN , 37232","place":["USA"]},{"name":"Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine , Nashville, TN ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Integrative Biology, University of California , Berkeley, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo F","family":"Rinaudo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of California San Francisco , San Francisco, CA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,5,31]]},"reference":[{"key":"2025091917110544000_ref1","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1152\/physrev.00029.2013","article-title":"Early developmental conditioning of later health and disease: physiology or pathophysiology?","volume":"94","author":"Hanson","year":"2014","journal-title":"Physiol Rev"},{"key":"2025091917110544000_ref2","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.earlhumdev.2006.07.001","article-title":"The Dutch famine and its long-term consequences for adult health","volume":"82","author":"Roseboom","year":"2006","journal-title":"Early Hum Dev"},{"key":"2025091917110544000_ref3","doi-asserted-by":"crossref","first-page":"E15","DOI":"10.1152\/ajpendo.00424.2017","article-title":"Epigenetics and developmental origins of diabetes: correlation or causation?","volume":"315","author":"Bansal","year":"2018","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"2025091917110544000_ref4","first-page":"9","article-title":"Animal models for DOHaD research","volume":"9","author":"Hsu","year":"2021","journal-title":"Focus on Hypertension of Developmental Origins Biomedicines"},{"key":"2025091917110544000_ref5","first-page":"4","article-title":"From embryos to adults: a DOHaD perspective on In vitro fertilization and other assisted reproductive technologies","volume":"4","author":"Feuer","year":"2016","journal-title":"Healthcare (Basel)"},{"key":"2025091917110544000_ref6","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1016\/S0140-6736(18)30312-X","article-title":"Origins of lifetime health around the time of conception: causes and consequences","volume":"391","author":"Fleming","year":"2018","journal-title":"Lancet"},{"key":"2025091917110544000_ref7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12958-016-0225-2","article-title":"Systematic review of worldwide trends in assisted reproductive technology 2004-2013","volume":"15","author":"Kushnir","year":"2017","journal-title":"Reprod Biol Endocrinol"},{"key":"2025091917110544000_ref8","first-page":"CD002118","article-title":"Cleavage-stage versus blastocyst-stage embryo transfer in assisted reproductive technology","volume":"2022","author":"Glujovsky","year":"2022","journal-title":"Cochrane Database Syst Rev"},{"key":"2025091917110544000_ref9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.fertnstert.2016.06.029","article-title":"Cleavage-stage or blastocyst transfer: what are the benefits and harms?","volume":"106","author":"Glujovsky","year":"2016","journal-title":"Fertil Steril"},{"key":"2025091917110544000_ref10","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s12978-020-0870-y","article-title":"Non-inferiority of cleavage-stage versus blastocyst-stage embryo transfer in poor prognosis IVF patients (PRECiSE trial): study protocol for a randomized controlled trial","volume":"17","author":"Neuhausser","year":"2020","journal-title":"Reprod Health"},{"key":"2025091917110544000_ref11","volume-title":"In: American Society of Reproductive Medicine","author":"Rinaudo","year":"2003"},{"key":"2025091917110544000_ref12","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1016\/j.fertnstert.2006.05.017","article-title":"Effects of oxygen tension on gene expression in preimplantation mouse embryos","volume":"86","author":"Rinaudo","year":"2006","journal-title":"Fertil Steril"},{"key":"2025091917110544000_ref13","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/humrep\/dez011","article-title":"Oxygen concentration alters mitochondrial structure and function in in vitro fertilized preimplantation mouse embryos","volume":"34","author":"Belli","year":"2019","journal-title":"Hum Reprod"},{"key":"2025091917110544000_ref14","doi-asserted-by":"crossref","first-page":"11","DOI":"10.7554\/eLife.79153","article-title":"Murine blastocysts generated by in vitro fertilization show increased Warburg metabolism and altered lactate production","volume":"11","author":"Lee","year":"2022","journal-title":"Elife"},{"key":"2025091917110544000_ref15","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1093\/molehr\/gaaa064","article-title":"Sex-specific epigenetic profile of inner cell mass of mice conceived In vivo or by In-vitro fertilization","volume":"26","author":"Ruggeri","year":"2020","journal-title":"Mol Hum Reprod"},{"key":"2025091917110544000_ref16","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1095\/biolreprod62.6.1526","article-title":"Differential effects of culture on imprinted H19 expression in the preimplantation mouse embryo","volume":"62","author":"Doherty","year":"2000","journal-title":"Biol Reprod"},{"key":"2025091917110544000_ref17","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1210\/en.2016-1475","article-title":"Assisted reproductive technologies predispose to insulin resistance and obesity in male mice challenged with a high-fat diet","volume":"158","author":"Cerny","year":"2017","journal-title":"Endocrinology"},{"key":"2025091917110544000_ref18","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1095\/biolreprod.113.113134","article-title":"In vitro fertilization affects growth and glucose metabolism in a sex-specific manner in an outbred mouse model","volume":"90","author":"Donjacour","year":"2014","journal-title":"Biol Reprod"},{"key":"2025091917110544000_ref19","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1210\/en.2013-2081","article-title":"Use of a mouse in vitro fertilization model to understand the developmental origins of health and disease hypothesis","volume":"155","author":"Feuer","year":"2014","journal-title":"Endocrinology"},{"key":"2025091917110544000_ref20","doi-asserted-by":"crossref","first-page":"5449","DOI":"10.1073\/pnas.0610317104","article-title":"Mouse embryo culture induces changes in postnatal phenotype including raised systolic blood pressure","volume":"104","author":"Watkins","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2025091917110544000_ref21","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1093\/humrep\/deaa205","article-title":"The duration of embryo culture after mouse IVF differentially affects cardiovascular and metabolic health in male offspring","volume":"35","author":"Aljahdali","year":"2020","journal-title":"Hum Reprod"},{"key":"2025091917110544000_ref22","first-page":"259","article-title":"Ovulation induction is associated with altered growth but with preservation of normal metabolic function in murine offspring","volume":"2","author":"Harner","year":"2021","journal-title":"F S Sci"},{"key":"2025091917110544000_ref23","first-page":"E353","article-title":"Dose-response curves for in vivo insulin sensitivity in individual tissues in rats","volume":"248","author":"Kraegen","year":"1985","journal-title":"Am J Physiol"},{"key":"2025091917110544000_ref24","first-page":"255","article-title":"Murine pancreatic islet isolation","author":"Szot","year":"2007","journal-title":"J Vis Exp"},{"key":"2025091917110544000_ref25","doi-asserted-by":"crossref","first-page":"101707","DOI":"10.1016\/j.molmet.2023.101707","article-title":"The long-lasting shadow of litter size in rodents: litter size is an underreported variable that strongly determines adult physiology","volume":"71","author":"Parra-Vargas","year":"2023","journal-title":"Mol Metab"},{"key":"2025091917110544000_ref26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/ilar.47.1.5","article-title":"Design and statistical methods in studies using animal models of development","volume":"47","author":"Festing","year":"2006","journal-title":"ILAR J"},{"key":"2025091917110544000_ref27","first-page":"6975","article-title":"The cumulative effect of assisted reproduction procedures on placental development and epigenetic perturbations in a mouse model","volume":"24","author":"de Waal","year":"2015","journal-title":"Hum Mol Genet"},{"key":"2025091917110544000_ref28","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1242\/dev.186551","article-title":"Assisted reproductive technologies induce temporally specific placental defects and the preeclampsia risk marker sFLT1 in mouse","volume":"147","author":"Vrooman","year":"2020","journal-title":"Development"},{"key":"2025091917110544000_ref29","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.cmet.2018.06.019","article-title":"CalR: a web-based analysis tool for indirect calorimetry experiments","volume":"28","author":"Mina","year":"2018","journal-title":"Cell Metab"},{"key":"2025091917110544000_ref30","first-page":"882","article-title":"Practice Committee of the American Society for reproductive medicine. Electronic address Aao, practice Committee of the American Society for reproductive M. Performing the embryo transfer: a guideline","volume":"107","year":"2017","journal-title":"Fertil Steril"},{"key":"2025091917110544000_ref31","doi-asserted-by":"crossref","first-page":"439","DOI":"10.2147\/DMSO.S234665","article-title":"Guidelines and considerations for metabolic tolerance tests in mice","volume":"13","author":"Benede-Ubieto","year":"2020","journal-title":"Diabetes Metab Syndr Obes"},{"key":"2025091917110544000_ref32","doi-asserted-by":"crossref","first-page":"20","DOI":"10.3390\/ijms20184382","article-title":"Maternal exposure to bisphenol a combined with high-fat diet-induced programmed hypertension in adult male rat offspring: effects of resveratrol","volume":"20","author":"Hsu","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2025091917110544000_ref33","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/978-90-481-3271-3_5","article-title":"High fat programming of beta-cell failure","volume":"654","author":"Cerf","year":"2010","journal-title":"Adv Exp Med Biol"},{"key":"2025091917110544000_ref34","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3389\/fendo.2013.00037","article-title":"Beta cell dysfunction and insulin resistance","volume":"4","author":"Cerf","year":"2013","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2025091917110544000_ref35","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.reprotox.2017.03.005","article-title":"Altered helper T cell-mediated immune responses in male mice conceived through in vitro fertilization","volume":"69","author":"Karimi","year":"2017","journal-title":"Reprod Toxicol"},{"key":"2025091917110544000_ref36","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1093\/humrep\/dead238","article-title":"Trophectoderm biopsy of blastocysts following IVF and embryo culture increases epigenetic dysregulation in a mouse model","volume":"39","author":"Rhon-Calderon","year":"2024","journal-title":"Hum Reprod"},{"key":"2025091917110544000_ref37","doi-asserted-by":"crossref","first-page":"e113155","DOI":"10.1371\/journal.pone.0113155","article-title":"Impaired glucose metabolism in response to high fat diet in female mice conceived by in vitro fertilization (IVF) or ovarian stimulation alone","volume":"9","author":"Chen","year":"2014","journal-title":"PloS One"},{"key":"2025091917110544000_ref38","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/mrd.22970","article-title":"Long term effects of ART: what do animals tell us?","volume":"85","author":"Duranthon","year":"2018","journal-title":"Mol Reprod Dev"},{"key":"2025091917110544000_ref39","doi-asserted-by":"crossref","first-page":"11684","DOI":"10.1038\/s41598-020-68549-3","article-title":"Effects of weaning age and housing conditions on phenotypic differences in mice","volume":"10","author":"Bailoo","year":"2020","journal-title":"Sci Rep"},{"key":"2025091917110544000_ref40","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1161\/01.cir.0000437597.44550.5d","article-title":"Diagnosis and treatment of fetal cardiac disease: a scientific statement from the American Heart Association","volume":"129","author":"Donofrio","year":"2014","journal-title":"Circulation"},{"key":"2025091917110544000_ref41","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1097\/00006250-200511000-00061","article-title":"Practice ACoG, genetics ACo. ACOG Committee opinion #324: perinatal risks associated with assisted reproductive technology","volume":"106","author":"Practice ACoO","year":"2005","journal-title":"Obstet Gynecol"},{"key":"2025091917110544000_ref42","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1161\/CIRCULATIONAHA.113.001878","article-title":"Pathological ventricular remodeling: mechanisms: part 1 of 2","volume":"128","author":"Burchfield","year":"2013","journal-title":"Circulation"},{"key":"2025091917110544000_ref43","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.jcmg.2010.10.008","article-title":"Left ventricular remodeling in heart failure: current concepts in clinical significance and assessment","volume":"4","author":"Konstam","year":"2011","journal-title":"JACC Cardiovasc Imaging"},{"key":"2025091917110544000_ref44","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.ahj.2010.12.009","article-title":"Association of systolic blood pressure with mortality in patients with heart failure with reduced ejection fraction: a complex relationship","volume":"161","author":"Ather","year":"2011","journal-title":"Am Heart J"},{"key":"2025091917110544000_ref45","author":"Ruggeri","year":"2018","edition":"meeting E (ed.)"},{"key":"2025091917110544000_ref46","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1073\/pnas.0306846101","article-title":"Long-term effects of culture of preimplantation mouse embryos on behavior","volume":"101","author":"Ecker","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2025091917110544000_ref47","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1530\/rep.1.00872","article-title":"Embryo culture does not affect the longevity of offspring in mice","volume":"130","author":"Sommovilla","year":"2005","journal-title":"Reproduction"},{"key":"2025091917110544000_ref48","doi-asserted-by":"crossref","first-page":"5052","DOI":"10.1172\/JCI68943","article-title":"Mice generated by in vitro fertilization exhibit vascular dysfunction and shortened life span","volume":"123","author":"Rexhaj","year":"2013","journal-title":"J Clin Invest"},{"key":"2025091917110544000_ref49","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1101\/gr.5217506","article-title":"Tissue-specific expression and regulation of sexually dimorphic genes in mice","volume":"16","author":"Yang","year":"2006","journal-title":"Genome Res"},{"key":"2025091917110544000_ref50","doi-asserted-by":"crossref","first-page":"4554","DOI":"10.1210\/en.2014-1465","article-title":"Sexually dimorphic effect of in vitro fertilization (IVF) on adult mouse fat and liver metabolomes","volume":"155","author":"Feuer","year":"2014","journal-title":"Endocrinology"},{"key":"2025091917110544000_ref51","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1111\/dom.14811","article-title":"The glucose tolerance test in mice: sex, drugs and protocol","volume":"24","author":"Kennard","year":"2022","journal-title":"Diabetes Obes Metab"},{"key":"2025091917110544000_ref52","doi-asserted-by":"crossref","first-page":"e13995","DOI":"10.14814\/phy2.13995","article-title":"The impact of age and sex on body composition and glucose sensitivity in C57BL\/6J mice","volume":"7","author":"Reynolds","year":"2019","journal-title":"Physiol Rep"},{"key":"2025091917110544000_ref53","first-page":"R39","article-title":"Early sex-dependent differences in response to environmental stress","volume":"155","author":"Perez-Cerezales","year":"2018","journal-title":"Reproduction"},{"key":"2025091917110544000_ref54","doi-asserted-by":"crossref","first-page":"19351","DOI":"10.1073\/pnas.0707258104","article-title":"DNA methylation, insulin resistance, and blood pressure in offspring determined by maternal periconceptional B vitamin and methionine status","volume":"104","author":"Sinclair","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2025091917110544000_ref55","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1071\/RD16265","article-title":"Sex-specific differences and developmental programming for diseases in later life","volume":"29","author":"Sundrani","year":"2017","journal-title":"Reprod Fertil Dev"},{"key":"2025091917110544000_ref56","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1080\/19396368.2020.1754961","article-title":"The effect of low and ultra-low oxygen tensions on mammalian embryo culture and development in experimental and clinical IVF","volume":"66","author":"Belli","year":"2020","journal-title":"Syst Biol Reprod Med"},{"key":"2025091917110544000_ref57","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1159\/000500071","article-title":"Effect of circadian rhythm on metabolic processes and the regulation of energy balance","volume":"74","author":"Serin","year":"2019","journal-title":"Ann Nutr Metab"},{"key":"2025091917110544000_ref58","first-page":"17","article-title":"Nonsurgical embryo transfer device compared with surgery for embryo transfer in mice","volume":"52","author":"Steele","year":"2013","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"2025091917110544000_ref59","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/biolre\/iox067","article-title":"The superovulated environment, independent of embryo vitrification, results in low birthweight in a mouse model","volume":"97","author":"Weinerman","year":"2017","journal-title":"Biol Reprod"},{"key":"2025091917110544000_ref60","doi-asserted-by":"crossref","DOI":"10.1101\/2024.02.06.579096","article-title":"Longitudinal fragility phenotyping predicts lifespan and age-associated morbidity in C57BL\/6 and diversity outbred mice","author":"Luciano","year":"2024"},{"key":"2025091917110544000_ref61","doi-asserted-by":"crossref","first-page":"190574","DOI":"10.1098\/rsos.190574","article-title":"Contrasting characteristic behaviours among common laboratory mouse strains","volume":"6","author":"Sultana","year":"2019","journal-title":"R Soc Open Sci"},{"key":"2025091917110544000_ref62","doi-asserted-by":"crossref","first-page":"e080133","DOI":"10.1136\/bmj-2024-080133","article-title":"Cumulative live birth rate of a blastocyst versus cleavage stage embryo transfer policy during in vitro fertilisation in women with a good prognosis: multicentre randomised controlled trial","volume":"386","author":"Cornelisse","year":"2024","journal-title":"BMJ"},{"key":"2025091917110544000_ref63","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1093\/humrep\/deaa032","article-title":"Obstetric and perinatal risks in 4601 singletons and 884 twins conceived after fresh blastocyst transfers: a Nordic study from the CoNARTaS group","volume":"35","author":"Spangmose","year":"2020","journal-title":"Hum Reprod"},{"key":"2025091917110544000_ref64","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.devcel.2017.07.026","article-title":"Zygotic genome activation in vertebrates","volume":"42","author":"Jukam","year":"2017","journal-title":"Dev Cell"},{"key":"2025091917110544000_ref65","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.tem.2007.02.004","article-title":"The predictive adaptive response and metabolic syndrome: challenges for the hypothesis","volume":"18","author":"Rickard","year":"2007","journal-title":"Trends Endocrinol Metab"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioaf120\/63410851\/ioaf120.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/113\/3\/657\/63410851\/ioaf120.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/113\/3\/657\/63410851\/ioaf120.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T21:11:20Z","timestamp":1758316280000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/113\/3\/657\/8154397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,31]]},"references-count":65,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,5,31]]},"published-print":{"date-parts":[[2025,9,15]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioaf120","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,9]]},"published":{"date-parts":[[2025,5,31]]}}}