{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:37:42Z","timestamp":1781966262507,"version":"3.54.5"},"reference-count":132,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Adenomyosis occurs when endometrial glands and stroma grow within the uterine myometrium. As a clinically significant disease, adenomyosis causes substantial pelvic pain and heavy menstrual bleeding. It remains understudied due to a lack of translational research tools and model systems. This study aimed to develop a telomerase-transformed, epithelial-like cell line derived from the eutopic endometrium of a subject with focal adenomyosis. De-identified endometrial tissue was processed through mechanical and enzymatic digestion. Epithelial and stromal populations were separated by selective adhesion, followed by fluorescence-activated cell sorting using an epithelial cellular adhesion molecule (EpCAM). EpCAM+ cells were effectively immortalized with the human telomerase reverse transcriptase (TERT) gene. Analyses confirmed the cells were human, free of mycoplasma contamination, and exhibited a unique 16-marker short tandem repeat (STR) profile. Cytogenetic analysis of G-banded metaphase spreads revealed polyploidy with multiple chromosomal rearrangements. The cell line, designated tAEC21, expressed epithelial markers cytokeratin-5 and N-cadherin but not the stromal marker CD10. Cells responded strongly to tumor necrosis factor-alpha stimulation by upregulating interleukin-6, C-X-C motif chemokine ligand 8, C-C motif chemokine ligand 2, and mucin 1 gene expression. In a heterotypic, three-dimensional (3D) spheroid model, tAEC21 formed a biologically relevant structure by creating an epithelial shell around the stromal cell core.<\/jats:p>\n                  <jats:p>In both two-dimensional (2D) monolayer and 3D culture, tAEC21 cells responded to 17\u03b2-estradiol (E2) but did not respond to progesterone (P4), consistent with the expression of estrogen (ESR1) and progesterone (PGR) receptors. This new epithelial-like, adenomyosis-derived cell line, tAEC21, will be an impactful, biologically plausible research resource.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf255","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T13:07:42Z","timestamp":1763557662000},"page":"1258-1276","source":"Crossref","is-referenced-by-count":1,"title":["Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21"],"prefix":"10.1093","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7324-8427","authenticated-orcid":false,"given":"Yuliya","family":"Klymenko","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]},{"name":"Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8664-1794","authenticated-orcid":false,"given":"Jessica L","family":"Kersey","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]},{"name":"Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]},{"name":"Department of Biochemistry and Molecular Biology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2518-390X","authenticated-orcid":false,"given":"Hunter D","family":"Quigley","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-3971","authenticated-orcid":false,"given":"Shannon M","family":"Hawkins","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]},{"name":"Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]},{"name":"Department of Biochemistry and Molecular Biology, Indiana University School of Medicine , Indianapolis, IN ,","place":["United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"2026041501022013200_ref1","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1097\/01.AOG.0000428646.67925.9a","article-title":"Obstetricians ACo, Gynecologists. 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