{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T11:09:47Z","timestamp":1773745787619,"version":"3.50.1"},"reference-count":77,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Newton Fund RCUK-CONACyT","award":["I000\/726\/2016 FONCICYT\/49\/2016"],"award-info":[{"award-number":["I000\/726\/2016 FONCICYT\/49\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Maternal obesity (MO) predisposes male offspring to impaired fertility and premature aging of reproductive function. We hypothesized that postnatal high-fat diet (HF) from early childhood to adulthood (second hit) exacerbates MO programmed outcomes in male offspring reproductive capacity. Female Wistar rats ate chow (5%-fat (C)) or HF diet (25%-fat (MO)) from weaning through pregnancy and lactation. Male offspring were weaned on chow (C\/C and MO\/C) or HF diet (C\/HF and MO\/HF) and euthanized at postnatal-day 110. Scrotal temperature and epididymal fat were higher in C\/HF and MO\/HF. Testicles: malondialdehyde (MDA) concentrations were increased in MO\/HF, reactive oxygen species (ROS) rose in MO\/C and MO\/HF, superoxide dismutase (SOD) activity increased in C\/HF and MO\/HF, and glutathione peroxidase (GPx) activity was higher in C\/HF, MO\/C, and MO\/HF. Testicular mRNA expression of Nrf2, SOD-1, GPx-4, and Bax\/Bcl2 ratio was lower in MO\/HF. Sperm: MDA concentrations increased in MO\/HF, ROS concentrations were elevated in MO\/C and exacerbated in C\/HF and MO\/HF, SOD activity decreased in MO\/HF. Sperm motility decreased, while sperm abnormalities increased in C\/HF, MO\/C, and MO\/HF. DNA fragmentation: yellow-stained sperms (low-fragmentation) were increased in MO\/C and exacerbated in C\/HF and MO\/HF; C\/HF, MO\/C, and MO\/HF showed more orange-stained sperms (medium-fragmentation), but MO\/HF had the highest increase. MO\/HF exhibited an increase in red-stained sperms (high-fragmentation). MO\/HF had a decreased fertility rate. MO combined with postnatal environmental factors, has a negative impact on offspring health and leads to greater reproductive alterations in comparison with unhealthy postnatal dietary habits or the programming caused by MO.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf261","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T13:01:24Z","timestamp":1764421284000},"page":"1101-1114","source":"Crossref","is-referenced-by-count":0,"title":["Postnatal high-fat diet consumption by male rat offspring of obese mothers accentuates negative outcomes of reproductive function in young adults"],"prefix":"10.1093","volume":"114","author":[{"given":"Guadalupe L","family":"Rodr\u00edguez-Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda de la Reproducci\u00f3n, Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n , Mexico City 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