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This study elucidated the role of hsa_circ_0001550 in impairing embryo adhesion and evaluated recombinant human granulocyte colony-stimulating factor (rhG-CSF) as a therapeutic strategy. Overexpression of hsa_circ_0001550 in an in vitro adhesion model using Ishikawa cells reduced the adhesion capacity of BeWo or JAR spheroids and diminished the murine blastocyst implantation rate. Knocking down hsa_circ_0001550 increased the adhesive ability of BeWo spheroids. Crucially, administration of rhG-CSF downregulated hsa_circ_0001550 expression and upregulated homeobox A10 and leukemia inhibitory factor, thereby restoring the adhesive capacity. In an endometrial injury model, administration of rhG-CSF enhanced epithelial proliferation (as measured by Ki67), suppressed apoptosis (based on the TUNEL assay), and activated mesenchymal-to-epithelial transition pathways, ultimately improving the embryo implantation rate. Collectively, this study reveals that rhG-CSF improves implantation partly by downregulating hsa_circ_0001550. These findings provide new perspectives for understanding implantation mechanisms and developing therapeutic strategies.<\/jats:p>","DOI":"10.1093\/biolre\/ioaf288","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T12:45:20Z","timestamp":1766493920000},"page":"810-826","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant human granulocyte colony-stimulating factor improves implantation partly by downregulating Hsa_circ_0001550"],"prefix":"10.1093","volume":"114","author":[{"given":"Ting","family":"Wang","sequence":"first","affiliation":[{"name":"The First School of Clinical Medicine, Lanzhou University , Lanzhou, Gansu ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuyao","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Lanzhou University , Lanzhou, Gansu 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