{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:26:04Z","timestamp":1787048764057,"version":"build-2736575974"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sturgeons (order Acipenseriformes) represent one of the most ancient lineages of Actinopterygii. Presently, most sturgeon species are classified as critically endangered. In this study, a novel ovarian cell line which contained germline cells derived from the sterlet (Acipenser ruthenus) was established, designated as the ARO cell line. Germline cells in the cell line were identified using marker gene analysis, alkaline phosphatase activity assay and immunofluorescence staining. Transmission electron microscope observation showed that previtellogenic oocytes existed in the ARO cell line. Flow cytometry analysis indicated the cell line maintained meiotic competence to generate DNA reduced ploidy (diploid) oocytes. Under three-dimensional culture condition, germinal vesicle stage, germinal vesicle breakdown stage and meiosis I oocytes were found. Furthermore, the cell line exhibited sustained proliferation and self-organization into reconstituted ovaries (rOvaries). Transcriptome analysis of rOvaries revealed that rOvaries had formed cell junctions and normal intercellular signaling, and possessed normal endocrine function and oocyte-producing capacity. In summary, the established ARO cell line exhibited stable passaging over multiple generations in vitro, was capable of sustainably generating oocytes, and was expected to be applicable to the establishment of ovarian organoids.<\/jats:p>","DOI":"10.1093\/biolre\/ioag076","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T11:14:28Z","timestamp":1776510868000},"page":"565-581","source":"Crossref","is-referenced-by-count":1,"title":["Establishment of a germline cell-containing ovarian cell line capable of oocyte generation in vitro from sterlet\n                    <i>Acipenser ruthenus<\/i>"],"prefix":"10.1093","volume":"115","author":[{"given":"Ai","family":"Sun","sequence":"first","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]},{"name":"Key Laboratory of Sturgeon Genetics and Breeding, Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Dong","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaohui","family":"Tian","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailiang","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Hu","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Sciences & Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute , Beijing , Beijing,","place":["China"]},{"name":"Key Laboratory of Sturgeon Genetics and Breeding, Ministry of Agriculture and Rural Affairs , Hangzhou , Zhejiang Province,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"2026081805381132500_ref1","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1126\/science.adn8556","article-title":"Ship sturgeon extinction risk in the Caspian Sea","volume":"383","author":"Falahatkar","year":"2024","journal-title":"Science"},{"key":"2026081805381132500_ref2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1023\/A:1007370213924","article-title":"An overview of Acipenseriformes","volume":"48","author":"Bemis","year":"1997","journal-title":"Environ Biol Fishes"},{"key":"2026081805381132500_ref3","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.theriogenology.2014.12.010","article-title":"Isolation and transplantation of sturgeon early-stage germ cells","volume":"83","author":"P\u0161eni\u010dka","year":"2015","journal-title":"Theriogenology"},{"key":"2026081805381132500_ref4","first-page":"13","article-title":"Effects of luteinizing hormone releasing hormone A2 on gonad development in juvenile Amur sturgeon, Acipenser schrenckii, revealed by transcriptome profiling analysis","volume":"24","author":"Lv","year":"2022","journal-title":"Front Genet"},{"key":"2026081805381132500_ref5","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.theriogenology.2015.07.003","article-title":"Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene","volume":"84","author":"Linhartov\u00e1","year":"2015","journal-title":"Theriogenology"},{"key":"2026081805381132500_ref6","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.3390\/biology11071069","article-title":"Generation of a normal long-term-cultured chinese hook snout carp spermatogonial stem cell line capable of sperm production in vitro","volume":"11","author":"Chen","year":"2022","journal-title":"Biology"},{"key":"2026081805381132500_ref7","first-page":"536","article-title":"Establishment and characteristics of the spermatogonial stem cell line from the yellow river carp (Cyprinus carpio haematopterus)","volume":"14","author":"Zhou","year":"2025","journal-title":"Biology (Basel)"},{"key":"2026081805381132500_ref8","doi-asserted-by":"crossref","first-page":"e62660","DOI":"10.1371\/journal.pone.0062660","article-title":"Production of zebra fish offspring from cultured female germline stem cells","volume":"8","author":"Wong","year":"2013","journal-title":"PloS One"},{"key":"2026081805381132500_ref9","doi-asserted-by":"crossref","first-page":"6772","DOI":"10.3390\/ijms26146772","article-title":"The primary cultivation of oogonial stem cells from black rockfish (Sebastes schlegelii): morphology and transcriptome landscape","volume":"26","author":"Zhang","year":"2025","journal-title":"Int J Mol Sci"},{"key":"2026081805381132500_ref10","doi-asserted-by":"crossref","first-page":"5861","DOI":"10.3390\/ijms23115861","article-title":"Isolation and characterization of germline stem cells in protogynous hermaphroditic Monopterus albus","volume":"23","author":"Sun","year":"2022","journal-title":"Int J Mol Sci"},{"key":"2026081805381132500_ref11","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1038\/nprot.2013.090","article-title":"Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells","volume":"8","author":"Hayashi","year":"2013","journal-title":"Nat Protoc"},{"key":"2026081805381132500_ref12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1274\/jmor.31.70","article-title":"Generation of oocytes from mouse ES\/iPS cells","volume":"31","author":"Hayashi","year":"2014","journal-title":"J Mammalian Ova Res"},{"key":"2026081805381132500_ref13","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1038\/nprot.2017.070","article-title":"Reconstitution of mouse oogenesis in a dish from pluripotent stem cells","volume":"12","author":"Hayashi","year":"2017","journal-title":"Nat Protoc"},{"key":"2026081805381132500_ref14","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/nature20104","article-title":"Reconstitution in vitro of the entire cycle of the mouse female germ line","volume":"539","author":"Hikabe","year":"2016","journal-title":"Nature"},{"key":"2026081805381132500_ref15","doi-asserted-by":"crossref","first-page":"15680","DOI":"10.1038\/ncomms15680","article-title":"In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells","volume":"8","author":"Jung","year":"2017","journal-title":"Nat Commun"},{"key":"2026081805381132500_ref16","doi-asserted-by":"crossref","first-page":"121213","DOI":"10.1016\/j.biomaterials.2021.121213","article-title":"Generation of offspring-producing 3D ovarian organoids derived from female germline stem cells and their application in toxicological detection","volume":"279","author":"Li","year":"2021","journal-title":"Biomaterials"},{"key":"2026081805381132500_ref17","doi-asserted-by":"crossref","first-page":"eabe0237","DOI":"10.1126\/science.abe0237","article-title":"Generation of ovarian follicles from mouse pluripotent stem cells","volume":"373","author":"Yoshino","year":"2021","journal-title":"Science"},{"key":"2026081805381132500_ref18","doi-asserted-by":"crossref","first-page":"101544","DOI":"10.1016\/j.xpro.2022.101544","article-title":"Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells","volume":"3","author":"Ishikura","year":"2022","journal-title":"Star Protoc"},{"key":"2026081805381132500_ref19","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/jfb.13855","article-title":"Establishment of a novel testicular cell line from sterlet Acipenser ruthenus and evaluation of its applications","volume":"94","author":"Sun","year":"2019","journal-title":"J Fish Biol"},{"key":"2026081805381132500_ref20","first-page":"516","article-title":"Comparative methods of reading data derived from genetic diversity analysis by microsatellite loci in farmed starlet Acipenser ruthenus population","volume":"31","author":"Dong","year":"2016","journal-title":"J Dalian Ocean Univ"},{"key":"2026081805381132500_ref21","doi-asserted-by":"crossref","first-page":"8011","DOI":"10.1073\/pnas.0308668101","article-title":"Establishment of a normal medaka fish spermatogonial cell line capable of sperm production in vitro","volume":"101","author":"Hong","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026081805381132500_ref22","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1038\/ncb1869","article-title":"Production of offspring from a germline stem cell line derived from neonatal ovaries","volume":"11","author":"Zou","year":"2009","journal-title":"Nat Cell Biol"},{"key":"2026081805381132500_ref23","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/scrt39","article-title":"Role of Oct4 in maintaining and regaining stem cell pluripotency","volume":"1","author":"Shi","year":"2009","journal-title":"Stem Cell Res Ther"},{"key":"2026081805381132500_ref24","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/nm.2669","article-title":"Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women","volume":"18","author":"White","year":"2012","journal-title":"Nat Med"},{"key":"2026081805381132500_ref25","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/s41586-024-07700-w","article-title":"Clonal inactivation of TERT impairs stem cell competition","volume":"632","author":"Hasegawa","year":"2024","journal-title":"Nature"},{"key":"2026081805381132500_ref26","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.fertnstert.2023.11.012","article-title":"Telomere dynamics and reproduction","volume":"121","author":"Robinson","year":"2024","journal-title":"Fertil Steril"},{"key":"2026081805381132500_ref27","doi-asserted-by":"crossref","first-page":"dev187773","DOI":"10.1242\/dev.187773","article-title":"Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition","volume":"148","author":"Bertho","year":"2021","journal-title":"Development"},{"key":"2026081805381132500_ref28","doi-asserted-by":"crossref","first-page":"7443","DOI":"10.1073\/pnas.1104501108","article-title":"Licensing of gametogenesis, dependent on RNA binding protein DAZL, as a gateway to sexual differentiation of fetal germ cells","volume":"108","author":"Gill","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026081805381132500_ref29","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.scr.2017.04.008","article-title":"RNA-binding proteins in human oogenesis: balancing differentiation and self-renewal in the female fetal germline","volume":"21","author":"Rosario","year":"2017","journal-title":"Stem Cell Res"},{"key":"2026081805381132500_ref30","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s00441-007-0403-9","article-title":"Balbiani cytoplasm in oocytes of a primitive fish, the sturgeon Acipenser gueldenstaedtii, and its potential homology to the Balbiani body (mitochondrial cloud) of Xenopus laevis oocytes","volume":"329","author":"Zelazowska","year":"2007","journal-title":"Cell Tissue Res"},{"key":"2026081805381132500_ref31","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1002\/jmor.20723","article-title":"Previtellogenic and vitellogenic oocytes in ovarian follicles of cultured siberian sturgeon Acipenser baerii (Chondrostei, Acipenseriformes)","volume":"278","author":"\u017belazowska","year":"2017","journal-title":"J Morphol"},{"key":"2026081805381132500_ref32","doi-asserted-by":"crossref","first-page":"e21631","DOI":"10.1002\/jmor.21631","article-title":"Asymmetry in previtellogenic and early vitellogenic oocytes, ultrastructure of follicular cells and egg envelope in the pigmented sterlet, Acipenser ruthenus L. 1758 (Chondrostei, Acipenseriformes)","volume":"284","author":"\u017belazowska","year":"2023","journal-title":"J Morphol"},{"key":"2026081805381132500_ref33","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s11427-024-2996-1","article-title":"Single-nucleus RNA sequencing reveals the underlying roadmap of early gonadal differentiation in teleost","volume":"69","author":"Wang","year":"2026","journal-title":"Sci China Life Sci"},{"key":"2026081805381132500_ref34","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1089\/scd.2022.0284","article-title":"Characterization of oogonial stem cells in adult mouse ovaries with age and comparison to in silico data on human ovarian aging","volume":"32","author":"MacDonald","year":"2023","journal-title":"Stem Cells Dev"},{"key":"2026081805381132500_ref35","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1530\/rep.1.01074","article-title":"TGF-beta superfamily members and ovarian follicle development","volume":"132","author":"Knight","year":"2006","journal-title":"Reproduction"},{"key":"2026081805381132500_ref36","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.mce.2011.11.002","article-title":"Novel signaling mechanisms in the ovary during oocyte maturation and ovulation","volume":"356","author":"Conti","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"2026081805381132500_ref37","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1262\/jrd.2016-016","article-title":"Porcine oocyte maturation in vitro: role of cAMP and oocyte-secreted factors \u2013 A practical approach","volume":"62","author":"Appeltant","year":"2016","journal-title":"J Reprod Dev"},{"key":"2026081805381132500_ref38","first-page":"159","article-title":"Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality","volume":"14","author":"Gilchrist","year":"2008","journal-title":"Hum Reprod"},{"key":"2026081805381132500_ref39","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1095\/biolreprod.102.013029","article-title":"A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence","volume":"(68)5","author":"O'Brien","year":"2003","journal-title":"Biol Reprod"},{"key":"2026081805381132500_ref40","doi-asserted-by":"crossref","first-page":"17323","DOI":"10.1038\/srep17323","article-title":"In vitro follicle growth supports human oocyte meiotic maturation","volume":"5","author":"Xiao","year":"2015","journal-title":"Sci Rep"},{"key":"2026081805381132500_ref41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-8603-3_1","article-title":"Growth In vitro of granulosa cell-oocyte complexes of the mouse","volume":"1818","author":"El-Hayek","year":"2018","journal-title":"Methods Mol Biol"},{"key":"2026081805381132500_ref42","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1093\/biolre\/ioac176","article-title":"Recapitulating folliculogenesis and oogenesis outside the body: encapsulated in vitro follicle growth","volume":"108","author":"Converse","year":"2023","journal-title":"Biol Reprod"},{"key":"2026081805381132500_ref43","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1002\/dvg.20784","article-title":"Somatic gonadal cells: the supporting cast for the germline","volume":"49","author":"Jemc","year":"2011","journal-title":"Genesis"},{"key":"2026081805381132500_ref44","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/nature02316","article-title":"Germline stem cells and follicular renewal in the postnatal mammalian ovary","volume":"428","author":"Johnson","year":"2004","journal-title":"Nature"},{"key":"2026081805381132500_ref45","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00441-005-0042-y","article-title":"Extracellular matrix in ovarian follicular development and disease","volume":"322","author":"Irving-Rodgers","year":"2005","journal-title":"Cell Tissue Res"},{"key":"2026081805381132500_ref46","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1007\/978-94-007-2888-2_49","article-title":"Calcium signaling in xenopus oocyte","volume":"740","author":"Marin","year":"2012","journal-title":"Adv Exp Med Biol"},{"key":"2026081805381132500_ref47","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1387\/ijdb.150239ss","article-title":"Calcium signals and oocyte maturation in marine invertebrates","volume":"59","author":"Deguchi","year":"2015","journal-title":"Int J Dev Biol"},{"key":"2026081805381132500_ref48","doi-asserted-by":"crossref","first-page":"1411000","DOI":"10.3389\/fendo.2024.1411000","article-title":"Calcium signaling in oocyte quality and functionality and its application","volume":"15","author":"Chen","year":"2024","journal-title":"Front Endocrinol (Lausanne)"},{"key":"2026081805381132500_ref49","doi-asserted-by":"crossref","first-page":"5225","DOI":"10.1242\/dev.02083","article-title":"Eph and NMDA receptors control Ca2+\/calmodulin-dependent protein kinase II activation during C. Elegans oocyte meiotic maturation","volume":"132","author":"Corrigan","year":"2005","journal-title":"Development"},{"key":"2026081805381132500_ref50","doi-asserted-by":"crossref","first-page":"4077","DOI":"10.1242\/dev.042143","article-title":"Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse","volume":"136","author":"Chang","year":"2009","journal-title":"Development"},{"key":"2026081805381132500_ref51","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1126\/science.adk5382","article-title":"Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction","volume":"384","author":"Liu","year":"2024","journal-title":"Science"},{"key":"2026081805381132500_ref52","doi-asserted-by":"crossref","first-page":"e2000734","DOI":"10.1002\/mnfr.202000734","article-title":"One carbon metabolism and mammalian pregnancy outcomes","volume":"65","author":"Cai","year":"2021","journal-title":"Mol Nutr Food Res"},{"key":"2026081805381132500_ref53","first-page":"12","article-title":"Folate supplementation in fertility and pregnancy: the advantages of (6S)5-Methyltetrahydrofolate","volume":"28","author":"Miraglia","year":"2022","journal-title":"Altern Ther Health Med"},{"key":"2026081805381132500_ref54","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1017\/S0967199421000356","article-title":"Modulating oxidative stress and epigenetic homeostasis in preimplantation IVF embryos","volume":"30","author":"Menezo","year":"2022","journal-title":"Zygote"},{"key":"2026081805381132500_ref55","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/978-3-319-60855-6_10","article-title":"DNA methyltransferases in mammalian oocytes","volume":"63","author":"Uysal","year":"2017","journal-title":"Results Probl Cell Differ"},{"key":"2026081805381132500_ref56","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nature14887","article-title":"The mechanism of DNA replication termination in vertebrates","volume":"525","author":"Dewar","year":"2015","journal-title":"Nature"},{"key":"2026081805381132500_ref57","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/ncb3500","article-title":"CUL-2LRR-1 and UBXN-3 drive replisome disassembly during DNA replication termination and mitosis","volume":"19","author":"Sonneville","year":"2017","journal-title":"Nat Cell Biol"},{"key":"2026081805381132500_ref58","doi-asserted-by":"crossref","first-page":"172795","DOI":"10.1016\/j.scitotenv.2024.172795","article-title":"Polystyrene nanoplastics cause reproductive toxicity in zebrafish: PPAR mediated lipid metabolism disorder","volume":"931","author":"Zheng","year":"2024","journal-title":"Sci Total Environ"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioag076\/68115091\/ioag076.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/115\/2\/565\/68115091\/ioag076.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/115\/2\/565\/68115091\/ioag076.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:38:24Z","timestamp":1787045904000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/115\/2\/565\/8659206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":58,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,20]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioag076","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,4,20]]}}}