{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:10:10Z","timestamp":1793045410340,"version":"4.5.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fondo Europeo de Desarrollo Regional","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["BFU2017-88407-P"],"award-info":[{"award-number":["BFU2017-88407-P"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001961","name":"AXA Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001961","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Clinica EUGIN"},{"name":"Epigenetic Effects of Ageing on Human Oocytes"},{"name":"Agencia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","award":["SGR 346"],"award-info":[{"award-number":["SGR 346"]}]},{"name":"Agencia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","award":["SGR 01222"],"award-info":[{"award-number":["SGR 01222"]}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["204352"],"award-info":[{"award-number":["204352"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union's Horizon 2020 research and innovation programme","award":["75442"],"award-info":[{"award-number":["75442"]}]},{"name":"ISCIII PT2020 Biobancos and Biomodelos call"},{"name":"Centro de Excelencia Severo Ochoa","award":["CEX2020-001049-S"],"award-info":[{"award-number":["CEX2020-001049-S"]}]},{"name":"Centro de Excelencia Severo Ochoa","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]},{"name":"Generalitat de Catalunya CERCA Programme"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The recent development of in vitro germ cell differentiation from pluripotent stem cells opened the human reproduction field to mechanistic investigations. In combination with induced pluripotent stem cell (hiPSC) reprogramming, in vitro gametogenesis provides an avenue for patient-specific disease modeling approaches. However, further advancements are required, as current protocols are limited to early stages of germ cell development. To get one step closer to this goal, here we aimed to identify optimal conditions for generating high-quality hiPSC lines capable of in vitro germ cell differentiation. We isolated two clinically available somatic cell types, peripheral blood-derived mononuclear cells and cumulus cells from the same female donor, and reprogrammed them into human induced pluripotent stem cells (hiPSCs). We then assessed their differentiation into ectoderm, mesoderm, and endoderm, and evaluated their X-chromosome inactivation status, a critical indicator of stem cell quality. Finally, we compared the capacity of PBMC- and cumulus-derived hiPSCs to differentiate into human primordial germ cell-like cells (hPGCLCs). We found that despite variability between cell lines, hiPSCs from both cell types were capable of generating hPGCLCs and that variations in X-chromosome state did not appear to generally interfere with the process. Our findings provide insight into germ cell differentiation from different clinically available starting materials guiding future patient-specific studies of fertility disorders.<\/jats:p>","DOI":"10.1093\/biolre\/ioag101","type":"journal-article","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T11:32:42Z","timestamp":1778412762000},"page":"634-645","source":"Crossref","is-referenced-by-count":1,"title":["Generation of human primordial germ cell-like cells from cumulus and blood cell-derived isogenic human induced pluripotent stem cells"],"prefix":"10.1093","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6190-0377","authenticated-orcid":false,"given":"Silvia","family":"Llonch","sequence":"first","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7964-2328","authenticated-orcid":false,"given":"Laurie","family":"Pinel","sequence":"additional","affiliation":[{"name":"Azrieli Research Centre, CHU Sainte-Justine, Montr\u00e9al , Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7174-6729","authenticated-orcid":false,"given":"Tom","family":"Mattimoe","sequence":"additional","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6959-0342","authenticated-orcid":false,"given":"Jacqueline","family":"Severino","sequence":"additional","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7275-2947","authenticated-orcid":false,"given":"Filippo","family":"Zambelli","sequence":"additional","affiliation":[{"name":"Basic Research Laboratory\u2014Eugin Group , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-0980","authenticated-orcid":false,"given":"Anna","family":"Mallol","sequence":"additional","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3342-2747","authenticated-orcid":false,"given":"Karen Freire","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Azrieli Research Centre, CHU Sainte-Justine, Montr\u00e9al , Qu\u00e9bec ,","place":["Canada"]},{"name":"Department of Pathology and Cell Biology, Universit\u00e9 de Montr\u00e9al , Montr\u00e9al, Qu\u00e9bec ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6924-9002","authenticated-orcid":false,"given":"Laura","family":"Batlle-Morera","sequence":"additional","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0846-0365","authenticated-orcid":false,"given":"Rita","family":"Vassena","sequence":"additional","affiliation":[{"name":"Basic Research Laboratory\u2014Eugin Group , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4694-2082","authenticated-orcid":false,"given":"Bernhard","family":"Payer","sequence":"additional","affiliation":[{"name":"Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology , Barcelona , Catalonia,","place":["Spain"]},{"name":"Azrieli Research Centre, CHU Sainte-Justine, Montr\u00e9al , Qu\u00e9bec ,","place":["Canada"]},{"name":"Department of Pathology and Cell Biology, Universit\u00e9 de Montr\u00e9al , Montr\u00e9al, Qu\u00e9bec ,","place":["Canada"]},{"name":"Universitat Pompeu Fabra (UPF) , Barcelona , Catalonia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"2026081805373038600_ref1","doi-asserted-by":"publisher","first-page":"eaaz6830","DOI":"10.1126\/science.aaz6830","article-title":"Mammalian in vitro 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Adv"}],"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioag101\/68267875\/ioag101.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/115\/2\/634\/68267875\/ioag101.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/article-pdf\/115\/2\/634\/68267875\/ioag101.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T09:37:39Z","timestamp":1787045859000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/article\/115\/2\/634\/8675877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,11]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,5,11]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioag101","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,8]]},"published":{"date-parts":[[2026,5,11]]}}}