{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:27:17Z","timestamp":1789655237374,"version":"4.0.1"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horizon 2020 research and innovation program \u201cFREIA\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endocrine disrupting chemicals (EDCs) impact female fertility, and their widespread presence demands deeper understanding of their effects on reproduction. In vitro maturation of bovine cumulus-oocyte complexes (COCs) serves as a relevant reproductive toxicology model aligned with the 3R principle. Given the link between lipid metabolism and COC quality, the possibility that lipid droplets (LDs) in cumulus cells (CCs) and overall COC lipid content are susceptible to EDC disruption was tested. COCs were exposed to diethylstilbestrol (DES; estrogen receptor agonist) and ketoconazole (KTZ; steroidogenesis inhibitor), during in vitro maturation. Neutral lipid dyes were used for LD visualization (count, size, area, and clustering) in CCs. Lipidomic analysis using liquid chromatography\u2013mass spectrometry was conducted separately on oocytes and CCs. Of all tested DES concentrations (i.e., 10\u22129\u00a0M, 10\u22127\u00a0M, 10\u22125\u00a0M), 10\u22125\u00a0M DES reduced the total LD area per CC, associated with decreased LD count and increased size. KTZ at all tested concentrations (i.e., 10\u22128\u00a0M, 10\u22127\u00a0M, 10\u22126\u00a0M) reduced LD size and enhanced clustering. Lipidomic analysis revealed the selective depletion of cholesteryl esters in CCs exposed to 10\u22125\u00a0M DES or 10\u22126\u00a0M KTZ, with minimal changes in other neutral lipid classes. Thus EDCs can disrupt lipid storage and LD dynamics in bovine COCs. We discuss these new findings with our previous work: (i) DES-induced steroidogenesis while (ii) in contrast KTZ blocked steroidogenesis in CCs. Together, the data highlight the COC lipid metabolism as a novel EDC target, warranting further study due to potential implications for female fertility.<\/jats:p>","DOI":"10.1093\/biolre\/ioag119","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:31:09Z","timestamp":1780399869000},"page":"968-985","source":"Crossref","is-referenced-by-count":1,"title":["The impact of endocrine disrupting chemicals diethylstilbestrol and ketoconazole on lipid storage in in vitro matured bovine cumulus-oocyte complexes"],"prefix":"10.1093","volume":"115","author":[{"given":"Konstantina","family":"Asimaki","sequence":"first","affiliation":[{"name":"Department Environmental Health and Toxicology, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam , Amsterdam ,","place":["Netherlands"]},{"name":"Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris H A","family":"Lest van de","sequence":"additional","affiliation":[{"name":"Department of Biomedical Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Wubbolts","sequence":"additional","affiliation":[{"name":"Department of Biomedical Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen W A","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Bernd","family":"Helms","sequence":"additional","affiliation":[{"name":"Department of Biomedical Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilde","family":"Aardema","sequence":"additional","affiliation":[{"name":"Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majorie B M","family":"Duursen van","sequence":"additional","affiliation":[{"name":"Department Environmental Health and Toxicology, Amsterdam Institute for Life and Environment, Vrije Universiteit Amsterdam , Amsterdam ,","place":["Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9618-1261","authenticated-orcid":false,"given":"Barend M","family":"Gadella","sequence":"additional","affiliation":[{"name":"Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht 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