{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T06:45:21Z","timestamp":1792046721835,"version":"4.4.0"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA1102000"],"award-info":[{"award-number":["2021YFA1102000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0805700"],"award-info":[{"award-number":["2021YFA0805700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202001BC070001"],"award-info":[{"award-number":["202001BC070001"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005273","name":"Natural Science Foundation of Yunnan Province","doi-asserted-by":"publisher","award":["202102AA100053"],"award-info":[{"award-number":["202102AA100053"]}],"id":[{"id":"10.13039\/501100005273","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U2102204"],"award-info":[{"award-number":["U2102204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Embryo transfer is a key application of assisted reproductive technology in non-human primates, underpinning research in reproductive biology, disease modeling, and conservation. The conventional approach\u2014tubal embryo transfer\u2014typically requires a laparoscopy system and abdominal incision to expose the oviducts and reproductive tract for embryo transfer, and is associated with complex procedures, a high cost, and cumulative reproductive tract injury following repeated surgeries. Moreover, implantation and pregnancy rates decline with increasing recipient age and surgical history, highlighting the need for a rapid, simple, and minimally invasive alternative. Here, using cynomolgus monkeys (Macaca fascicularis) as a model, we implemented a transabdominal uterine puncture method. This technique involves the direct introduction of in vitro cultured embryos into the uterine cavity by means of an ultrasound-guided needle. In a cohort of 37 recipients, transabdominal uterine puncture yielded confirmed implantation in 19 animals (51.35%). Importantly, implantation and pregnancy outcomes in older animals and in those with prior reproductive surgeries were substantially higher than rates historically observed with tubal embryo transfer. Gestation assessments and neonatal examinations revealed no discernible abnormalities or increased perinatal complications in offspring produced via transabdominal uterine puncture, compared with controls, supporting the method as an efficient and safe embryo transfer strategy for non-human primates.<\/jats:p>","DOI":"10.1093\/biolre\/ioag160","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:12:08Z","timestamp":1785237128000},"page":"1292-1298","source":"Crossref","is-referenced-by-count":0,"title":["Efficient production of cynomolgus monkeys via transabdominal uterine puncture embryo transfer"],"prefix":"10.1093","volume":"115","author":[{"given":"Zifan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinglong","family":"Chen","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baohong","family":"Tian","sequence":"additional","affiliation":[{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyang","family":"Si","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2550-4224","authenticated-orcid":false,"given":"Weizhi","family":"Ji","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8797-4152","authenticated-orcid":false,"given":"Yuyu","family":"Niu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1730-6498","authenticated-orcid":false,"given":"Yu","family":"Kang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology , Kunming ,","place":["China"]},{"name":"Yunnan Key Laboratory of Primate Biomedical Research , Kunming ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"2026101502073011000_ref1","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1038\/nature08090","article-title":"Generation of transgenic non-human primates with germline transmission","volume":"459","author":"Sasaki","year":"2009","journal-title":"Nature"},{"key":"2026101502073011000_ref2","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1002\/ajp.22281","article-title":"Why primate models matter","volume":"76","author":"Phillips","year":"2014","journal-title":"Am J Primatol"},{"key":"2026101502073011000_ref3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1126\/science.291.5502.309","article-title":"Transgenic monkeys produced by retroviral gene transfer into mature oocytes","volume":"291","author":"Chan","year":"2001","journal-title":"Science"},{"key":"2026101502073011000_ref4","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1002\/ajp.21017","article-title":"The available time window for embryo transfer in the rhesus monkey (Macaca mulatta)","volume":"74","author":"Chen","year":"2012","journal-title":"Am J Primatol"},{"key":"2026101502073011000_ref5","doi-asserted-by":"publisher","first-page":"17663","DOI":"10.1073\/pnas.1006563107","article-title":"Transgenic rhesus monkeys produced by gene transfer into early-cleavage\u2013stage embryos using a simian immunodeficiency virus-based vector","volume":"107","author":"Niu","year":"2010","journal-title":"Proc Natl Acad Sci"},{"key":"2026101502073011000_ref6","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1093\/humrep\/15.3.616","article-title":"Embryo transfer under ultrasound guidance improves pregnancy rates after in-vitro 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