{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T19:09:43Z","timestamp":1788721783505,"version":"build-2803163510"},"reference-count":99,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In vitro embryo production (IVP) has achieved remarkable gains in efficiency, yet these advances remain based on oversimplified models that prioritize blastocyst yield over biological quality. Embryos reaching the blastocyst stage in vitro frequently exhibit molecular and functional deviations with consequences for implantation, placental function, and offspring health. Omics studies indicate that non-physiological culture conditions during epigenetically vulnerable developmental windows can rewire regulatory architecture by altering metabolic flux, epigenetic landscapes, and gene expression networks. However, most studies remain descriptive, limiting mechanistic understanding. Advancing embryo quality requires functional perturbation, multi-omics integration, and mechanistically driven experimental design. Here, we propose a conceptual framework for next-generation IVP based on adaptive culture conditions responsive to embryo-derived signals, restoration of epigenetic integrity, and bidirectional embryo\u2013environment communication through oviductal signals, extracellular vesicles, and bioengineered interfaces. Success must shift from blastocyst formation toward functionally validated developmental outcomes.<\/jats:p>","DOI":"10.1093\/biolre\/ioag177","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T17:30:49Z","timestamp":1787851849000},"source":"Crossref","is-referenced-by-count":0,"title":["Making better embryos\u2014not more: omics-guided lessons to redesign in vitro systems"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-3066","authenticated-orcid":false,"given":"Marcella Pecora","family":"Milazzotto","sequence":"first","affiliation":[{"name":"Center of Natural and Human Science, Federal University of ABC , Santo Andre, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"2026090615065827600_ref1","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/S0140-6736(78)92957-4","article-title":"Birth after 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