{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:57:19Z","timestamp":1790542639848,"version":"4.1.0"},"reference-count":129,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infertility affects millions of couples worldwide, and male factors contribute to approximately 20\u201350% of infertility cases. Male infertility is typically evaluated with a semen analysis, which is a fundamental analysis to determine sperm quality. However, this analysis is limited. One of its most important deficiencies is the lack of functional sperm assessment. Because of this, semen analysis does not reveal information about the in vivo behavior of spermatozoa or their fertilization potential. This constitutes a concern because, in conventional semen analyses, 30% of infertile men display normal values in their semen analysis. In contrast, functional tests such as sperm-zona pellucida binding assay and acrosome exocytosis (AE, also known as acrosome reaction) test predict male fertilization potential with greater accuracy. Here, we review functional tests and their potential to improve male infertility diagnostics and their predictive value for fertilization rates after in vitro fertilization (IVF) intervention. We also discuss the advantages of AE as a functional test to improve the diagnosis of male infertility in humans and other species and its implementation in the clinic.<\/jats:p>","DOI":"10.1093\/biolre\/ioag196","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:20:21Z","timestamp":1789654821000},"source":"Crossref","is-referenced-by-count":0,"title":["The acrosome exocytosis as a diagnostic functional test: a narrative review"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4703-237X","authenticated-orcid":false,"given":"Jorge Arturo","family":"Torres Ju\u00e1rez","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Avanzada, Facultad de Medicina, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00edda Guadalupe","family":"D\u00edaz Martel","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Avanzada, Facultad de Medicina, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Laura","family":"Guti\u00e9rrez Silva","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Gen\u00e9tica, Facultad de Qu\u00edmica, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esperanza","family":"Mata Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular y Desarrollo, Instituto de Fisiolog\u00eda Celular, Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayd\u00e9","family":"Vergara Casta\u00f1eda","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Avanzada, Facultad de Medicina, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Trevi\u00f1o Santa Cruz","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda (IBT), Universidad Nacional Aut\u00f3noma de M\u00e9xico (UNAM), M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2671-2789","authenticated-orcid":false,"given":"Ana Alicia","family":"S\u00e1nchez Tusie","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica Avanzada, Facultad de Medicina, Universidad Aut\u00f3noma de Quer\u00e9taro, Quer\u00e9taro, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"2026092716215108100_ref1","volume-title":"WHO laboratory manual for the 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