{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:52:15Z","timestamp":1791557535194,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Direct experimental analysis of the mammalian oviduct is constrained by limited tissue access and the short lifespan of ex vivo preparations. Extracellular matrix-embedded three-dimensional epithelial organoids provide longer-term in vitro models. However, their inward-facing apical surface and the absence of supporting stromal cells limit physiological studies of the oviduct, including ciliary activity and maternal-embryonic interactions. Here, we provide a step-wise protocol detailing the generation of mouse and human oviductal assembloids in which epithelial cells form an outward-facing (apical-out) layer around a stromal core. Epithelial and stromal cells from adult mouse oviducts or human Fallopian tubes are isolated, expanded separately, and subsequently aggregated in a rotational culture system. The protocol also outlines morphological and immunostaining criteria for confirming cellular organization, whole-mount detection of external cilia, measurement of ciliary beat frequency, and co-culture of mouse assembloids with preimplantation embryos. Mouse and human assembloids retained epithelial and stromal identity and displayed cilia at the accessible outer surface. In a proof-of-concept experiment, embryos co-cultured with the assembloids developed to blastocysts at a rate similar to that of in vivo-derived blastocysts. This reductionist system provides a straightforward and tractable model to investigate oviduct physiology and embryo-maternal communication while allowing direct manipulation and observation of the epithelial interface.<\/jats:p>","DOI":"10.1093\/biolre\/ioag225","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:47:27Z","timestamp":1791546447000},"source":"Crossref","is-referenced-by-count":0,"title":["Method for modeling oviduct function and impact on embryonic development"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9323-3764","authenticated-orcid":false,"given":"Kalli K","family":"Stephens","sequence":"first","affiliation":[{"name":"Division of Animal Sciences, College of Agriculture, Food and Natural Resources"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5071-8289","authenticated-orcid":false,"given":"Vakil","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makenna K","family":"Shifflett","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0594-0571","authenticated-orcid":false,"given":"Jiude","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-1153","authenticated-orcid":false,"given":"Jason A","family":"Rizo","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, College of Agriculture, Food and Natural Resources"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8616-5003","authenticated-orcid":false,"given":"Mark I","family":"Hunter","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1484-4998","authenticated-orcid":false,"given":"Andrew M","family":"Kelleher","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5196-8471","authenticated-orcid":false,"given":"Wipawee","family":"Winuthayanon","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, College of Agriculture, Food and Natural Resources"},{"name":"Department of Obstetrics, Gynecology, and Women\u2019s Health"},{"name":"Department of Medical Pharmacology and Physiology, School of Medicine, University of Missouri , Columbia, Missouri , \u00a0 65211","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,9]]},"container-title":["Biology of Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioag225\/71788167\/ioag225.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article-pdf\/doi\/10.1093\/biolre\/ioag225\/71788167\/ioag225.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:24:41Z","timestamp":1791555881000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biolreprod\/advance-article\/doi\/10.1093\/biolre\/ioag225\/8898644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/biolre\/ioag225","relation":{},"ISSN":["0006-3363","1529-7268"],"issn-type":[{"value":"0006-3363","type":"print"},{"value":"1529-7268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]},"article-number":"ioag225"}}