{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T04:57:55Z","timestamp":1769749075671,"version":"3.49.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Numerous intrauterine changes take place across species during embryo development. Following fertilization in July\/August, the European roe deer (Capreolus capreolus) embryo undergoes diapause until embryonic elongation in December\/January. Embryonic elongation prior to implantation is a common feature among ungulates. Unlike many other ruminants, the roe deer embryo does not secrete interferon-tau (IFN\u03c4). This provides the unique opportunity to unravel IFN\u03c4-independent signaling pathways associated with maternal recognition of pregnancy (MRP). This study aimed at identifying the cell-type\u2013specific endometrial gene expression changes associated with the MRP at the time of embryo elongation that are independent of IFN\u03c4 in roe deer. The messenger RNA (mRNA) expression of genes known to be involved in embryo\u2013maternal communication in cattle, pig, sheep, and mice was analyzed in laser capture microdissected (LMD) endometrial luminal, glandular epithelial, as well as stromal cells. The mRNA transcript abundances of the estrogen (ESR1), progesterone receptor (PGR), and IFN\u03c4-stimulated genes were lower in the luminal epithelium in the presence of an elongated embryo compared to diapause. Retinol Binding Protein-4 (RBP4), a key factor involved in placentation, was more abundant in the luminal epithelium in the presence of an elongated embryo. The progesterone receptor localization was visualized by immunohistochemistry, showing an absence in the luminal epithelium and an overall lower abundance with time and thus prolonged progesterone exposure. Our data show a developmental stage-specific mRNA expression pattern in the luminal epithelium, indicating that these cells sense the presence of an elongated embryo in an IFN\u03c4-independent manner.<\/jats:p>","DOI":"10.1093\/biolre\/ioz129","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T08:46:08Z","timestamp":1563439568000},"source":"Crossref","is-referenced-by-count":4,"title":["Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau\u2020"],"prefix":"10.1093","author":[{"given":"Vera A","family":"van der Weijden","sequence":"first","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brina","family":"Puntar","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Rudolf Vegas","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Milojevic","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corina I","family":"Schanzenbach","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariusz P","family":"Kowalewski","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Drews","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne E","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"2019101010283920400_ref1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/2049-1891-4-23","article-title":"Pregnancy recognition signaling mechanisms in ruminants and pigs","volume":"4","author":"Bazer","year":"2013","journal-title":"J Anim Sci 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