{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:48:45Z","timestamp":1761598125403},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T00:00:00Z","timestamp":1570665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In our earlier work, we found that intrauterine (i.u.) and intraperitoneal (i.p.) injection of LPS (10-\u03bcg serotype 0111:B4) induced preterm labor (PTL) with high pup mortality, marked systemic inflammatory response and hypotension. Here, we used both i.u. and i.p. LPS models in pregnant wild-type (wt) and CCR2 knockout (CCR2\u2212\/\u2212) mice on E16 to investigate the role played by the CCL2\/CCR2 system in the response to LPS.<\/jats:p>\n               <jats:p>Basally, lower numbers of monocytes and macrophages and higher numbers of neutrophils were found in the myometrium, placenta, and blood of CCR2\u2212\/\u2212 vs. wt mice. After i.u. LPS, parturition occurred at 14\u00a0h in both groups of mice. At 7\u00a0h post-injection, 70% of wt pups were dead vs. 10% of CCR2\u2212\/\u2212 pups, but at delivery 100% of wt and 90% of CCR2\u2212\/\u2212 pups were dead. Myometrial and placental monocytes and macrophages were generally lower in CCR2\u2212\/\u2212 mice, but this was less consistent in the circulation, lung, and liver. At 7\u00a0h post-LPS, myometrial ERK activation was greater and JNK and p65 lower and the mRNA levels of chemokines were higher and of inflammatory cytokines lower in CCR2\u2212\/\u2212 vs. wt mice. Pup brain and placental inflammation were similar. Using the IP LPS model, we found that all measures of arterial pressure increased in CCR2\u2212\/\u2212 but declined in wt mice.<\/jats:p>\n               <jats:p>These data suggest that the CCL2\/CCR2 system plays a critical role in the cardiovascular response to LPS and contributes to pup death but does not influence the onset of inflammation-induced PTL.<\/jats:p>","DOI":"10.1093\/biolre\/ioz188","type":"journal-article","created":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T17:54:59Z","timestamp":1570730099000},"page":"445-455","source":"Crossref","is-referenced-by-count":3,"title":["CCR2 mediates the adverse effects of LPS in the pregnant mouse"],"prefix":"10.1093","volume":"102","author":[{"given":"Renyi","family":"Hua","sequence":"first","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"},{"name":"The International Peace Maternity & Child Health Hospital of China Welfare Institute (IPMCH), School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai Municipal Key Clinical Specialty, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia F","family":"Edey","sequence":"additional","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieran P","family":"O\u2019Dea","sequence":"additional","affiliation":[{"name":"Section of Anaesthetics, Pain Medicine, and Intensive Care, Faculty of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Howe","sequence":"additional","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bronwen R","family":"Herbert","sequence":"additional","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwei","family":"Cheng","sequence":"additional","affiliation":[{"name":"The International Peace Maternity & Child Health Hospital of China Welfare Institute (IPMCH), School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai Municipal Key Clinical Specialty, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Zheng","sequence":"additional","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A","family":"MacIntyre","sequence":"additional","affiliation":[{"name":"Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip R","family":"Bennett","sequence":"additional","affiliation":[{"name":"Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Takata","sequence":"additional","affiliation":[{"name":"The International Peace Maternity & Child Health Hospital of China Welfare Institute (IPMCH), School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai Municipal Key Clinical Specialty, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R","family":"Johnson","sequence":"additional","affiliation":[{"name":"Imperial College Parturition Research Group, Academic Department of Obstetrics & Gynaecology, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,10,10]]},"reference":[{"key":"2020021302232572400_ref1","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1016\/S0140-6736(12)60820-4","article-title":"National, regional, and worldwide estimates of preterm birth rates in the year 2010 with time trends since 1990 for selected countries: a systematic analysis and 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