{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:46:51Z","timestamp":1790812011189,"version":"4.1.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T00:00:00Z","timestamp":1588377600000},"content-version":"vor","delay-in-days":122,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"NIH\/NIAID","award":["K08 AI135072"],"award-info":[{"award-number":["K08 AI135072"]}]},{"name":"Burroughs Wellcome","award":["CAMS 1017213"],"award-info":[{"award-number":["CAMS 1017213"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dried blood spots (DBS) are widely utilized as part of universal newborn screening and as a means of transporting samples from field sites. We use DBS from African field sites to assess for rare maternal-fetal cell exchange during pregnancy known as microchimerism. We aimed to develop a protocol to maximize the quantity of high-quality genomic DNA (gDNA) extracted from DBS. The total gDNA yield obtained from control DBS utilizing a Qiagen-based protocol and a Chelex\u00ae 100 resin-based protocol was first compared. Variations of the Chelex\u00ae protocol were subsequently tested to develop an optimized protocol. The gDNA was quantified by qPCR targeting the human beta-globin gene. DNA yield for a given experimental condition was normalized to a Chelex\u00ae control performed on the same day, and the total yields were compared using a Student\u2019s t-test. The control Chelex\u00ae protocol yielded 590% more DNA than the QIAamp\u00ae DNA Blood Mini Kit . The absolute efficiency of the control Chelex\u00ae protocol was 54%, compared to an absolute efficiency of 9% for the QIAamp\u00ae DNA Blood Mini Kit. Modification of the Chelex\u00ae protocol to include a second heat precipitation from the same DBS increased the gDNA yield by 29% (P\u2009&amp;lt;\u20090.001). Our optimized protocol including this modification increased the absolute efficiency of extraction to 68%. The gDNA extracted using the Chelex\u00ae protocol was stable through repeated freeze\u2013thaw cycles. In a mock microchimerism experiment, rare donor alleles at a frequency of 10 in 100\u00a0000 could be identified in gDNA from DBS extracted using the optimized Chelex\u00ae protocol. Our findings may be of significance for a diverse range of applications that utilize DBS and require high-quality DNA, including newborn screening programs, pathogen and drug resistance screening from remote field sites, forensics, and rare allele detection.<\/jats:p>","DOI":"10.1093\/biomethods\/bpaa009","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T11:09:15Z","timestamp":1587726555000},"source":"Crossref","is-referenced-by-count":51,"title":["Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots"],"prefix":"10.1093","volume":"5","author":[{"given":"Neta","family":"Simon","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaclyn","family":"Shallat","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey","family":"Williams Wietzikoski","sequence":"first","affiliation":[{"name":"Seattle Children\u2019s Research Institute, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0121-978X","authenticated-orcid":false,"given":"Whitney E","family":"Harrington","sequence":"first","affiliation":[{"name":"Seattle Children\u2019s Research Institute, Seattle, WA, USA"},{"name":"Department of Pediatrics, University of Washington, Seattle, WA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,5,2]]},"reference":[{"key":"2020070408540223600_bpaa009-B1","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1373\/clinchem.2017.275966","article-title":"State of the science in dried blood spots","volume":"64","author":"Freeman","year":"2018","journal-title":"Clin 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