{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:49:09Z","timestamp":1790912949910,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T00:00:00Z","timestamp":1602115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Dutch Ministry of Defence","award":["V1802"],"award-info":[{"award-number":["V1802"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fluorescence-based diagnostic tools are attractive and versatile tests with multiple advantages: ease of use, sensitivity and rapid results. The advent of CRISPR-Cas technology has created new avenues for the development of diagnostic testing tools. In this study, by effectively combining the specific functions of two enzymes, CRISPR-Cas12a and terminal deoxynucleotidyl transferase (TdT), we developed a DNA detection assay that generates copper nanoparticles (CuNPs) that are easily visible to the naked eye under UV-light; we named this detection assay Cas12a Activated Nuclease poly-T Reporter Illuminating Particles (CANTRIP). Upon specific target DNA recognition by Cas12a, single-stranded DNA (ssDNA) reporter oligos with blocked 3\u2032-ends are cut into smaller ssDNA fragments, thereby generating neo 3\u2032-hydroxyl moieties. TdT subsequently elongates these newly formed ssDNA fragments, incorporating only dTTP nucleotides, and these poly(thymine)-tails subsequently function as scaffolds for the formation of CuNPs. These CuNPs produce a bright fluorescent signal upon UV excitation, and thus, this bright orange signal indicates the presence of target DNA, which in this proof-of-concept study consisted of anthrax lethal factor plasmid DNA. CANTRIP, which combines two detection platforms consisting of CRISPR-Cas12a and fluorescent CuNPs into a single reaction, appears to be a robust, low-cost and simple diagnostic tool.<\/jats:p>","DOI":"10.1093\/biomethods\/bpaa020","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T19:14:27Z","timestamp":1602098067000},"source":"Crossref","is-referenced-by-count":30,"title":["Bright fluorescent nucleic acid detection with CRISPR-Cas12a and poly(thymine) templated copper nanoparticles"],"prefix":"10.1093","volume":"6","author":[{"given":"Janna F M","family":"Bogers","sequence":"first","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8204-3941","authenticated-orcid":false,"given":"Nicole F","family":"Berghuis","sequence":"additional","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruud W","family":"Busker","sequence":"additional","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"van Booma","sequence":"additional","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armand","family":"Paauw","sequence":"additional","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans C","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Department of CBRN Protection, Netherlands Organization for Applied Scientific Research TNO, 2288 GJ, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"2021082115095489300_bpaa020-B1","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.tibtech.2018.12.005","article-title":"CRISPR\/Cas systems towards next-generation biosensing","volume":"37","author":"Li","year":"2019","journal-title":"Trends Biotechnol"},{"key":"2021082115095489300_bpaa020-B2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1126\/science.aaf5573","article-title":"C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR 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