{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:38:56Z","timestamp":1792571936330,"version":"4.5.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":140,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"publisher","award":["113Z371"],"award-info":[{"award-number":["113Z371"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Doxorubicin (DOX) is a widely used chemotherapeutic agent that induces apoptosis through DNA intercalation, topoisomerase II inhibition, and oxidative stress. However, its cardiotoxicity is a significant concern. Flow cytometry, particularly with Annexin V\/propidium iodide (PI) or Annexin V\/7AAD assays, is commonly used to assess DOX-induced cell death. A challenge arises from DOX\u2019s intrinsic fluorescence, which interferes with accurate detection. To address this, we successfully used DRAQ7, a far-red dye, in HeLa and other cancer cell lines. As our study demonstrates DRAQ7 eliminates auto-fluorescence interference, this approach offers precise differentiation of apoptotic cells and improving experimental and therapeutic reliability.<\/jats:p>","DOI":"10.1093\/biomethods\/bpag025","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:23:30Z","timestamp":1779103410000},"source":"Crossref","is-referenced-by-count":0,"title":["DRAQ7 overcomes the complications associated with autofluorescence of doxorubicin during flow cytometric analyses"],"prefix":"10.1093","volume":"11","author":[{"given":"Yusuf Cem","family":"\u00c7ift\u00e7i","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Izmir Institute of Technology , Izmir, 35430,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130pek","family":"Erdo\u011fan Vatansever","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Izmir Institute of Technology , Izmir, 35430,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9877-9689","authenticated-orcid":false,"given":"B\u00fcnyamin","family":"Akg\u00fcl","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Izmir Institute of Technology , Izmir, 35430,","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"2026102103474834200_bpag025-B1","doi-asserted-by":"publisher","first-page":"15","DOI":"10.2165\/00003088-198815010-00002","article-title":"Clinical pharmacokinetics of doxorubicin","volume":"15","author":"Speth","year":"1988","journal-title":"Clin Pharmacokinet"},{"key":"2026102103474834200_bpag025-B2","doi-asserted-by":"publisher","first-page":"760","DOI":"10.7150\/ijbs.65258","article-title":"Underlying the mechanisms of doxorubicin-\u0131nduced acute cardiotoxicity: oxidative stress and cell death","volume":"18","author":"Kong","year":"2022","journal-title":"Int J Biol Sci"},{"key":"2026102103474834200_bpag025-B3","doi-asserted-by":"publisher","first-page":"853","DOI":"10.4103\/0973-1482.139267","article-title":"An overview of doxorubicin formulations in cancer therapy","volume":"10","author":"Rivankar","year":"2014","journal-title":"J Cancer Res Ther"},{"key":"2026102103474834200_bpag025-B4","doi-asserted-by":"publisher","first-page":"3267","DOI":"10.2174\/092986709788803312","article-title":"Doxorubicin: the good, the bad and the ugly effect","volume":"16","author":"Carvalho","year":"2009","journal-title":"Curr Med Chem"},{"key":"2026102103474834200_bpag025-B5","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/s41420-023-01565-0","article-title":"Nrf2: a dark horse in doxorubicin-induced cardiotoxicity","volume":"9","author":"Zhao","year":"2023","journal-title":"Cell Death Discov"},{"key":"2026102103474834200_bpag025-B6","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1038\/s41419-021-03614-x","article-title":"Regulated cell death pathways in doxorubicin-induced cardiotoxicity","volume":"12","author":"Christidi","year":"2021","journal-title":"Cell Death Dis"},{"key":"2026102103474834200_bpag025-B7","doi-asserted-by":"publisher","first-page":"181","DOI":"10.4155\/bio-2020-0267","article-title":"Flow cytometry: principles, applications and recent advances","volume":"13","author":"Manohar","year":"2021","journal-title":"Bioanalysis"},{"key":"2026102103474834200_bpag025-B8","doi-asserted-by":"publisher","first-page":"5.1.1","DOI":"10.1002\/cpim.40","article-title":"Flow Cytometry: an overview","volume":"120","author":"McKinnon","year":"2018","journal-title":"Curr Protoc Immunol"},{"key":"2026102103474834200_bpag025-B9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-61737-950-5_1","article-title":"Flow cytometry: an introduction","volume":"699","author":"Givan","year":"2011","journal-title":"Methods Mol Biol"},{"key":"2026102103474834200_bpag025-B10","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot087288","article-title":"Quantitation of apoptosis and necrosis by annexin V binding, propidium \u0131odide uptake, and flow cytometry","volume":"953\u201357","author":"Crowley","year":"2016","journal-title":"Cold Spring Harb Protoc"},{"key":"2026102103474834200_bpag025-B11","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ab.2012.07.005","article-title":"7-Aminoactinomycin D for apoptosis staining in flow cytometry","volume":"429","author":"Zembruski","year":"2012","journal-title":"Anal Biochem"},{"key":"2026102103474834200_bpag025-B12","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1385\/1-59259-380-1:323","article-title":"Flow cytometric methods for detection and quantification of apoptosis","volume":"85","author":"Steensma","year":"2003","journal-title":"Methods Mol Med"},{"key":"2026102103474834200_bpag025-B13","first-page":"1","article-title":"Non-coding RNAs in apoptosis: identification and function","volume":"46","author":"T\u00fcncel","year":"2022","journal-title":"Turkish J Biol"},{"key":"2026102103474834200_bpag025-B14","first-page":"1703","article-title":"Doxorubicin gradients in human breast cancer","volume":"5","author":"Lankelma","year":"1999","journal-title":"Clin Cancer Res an off J Am Assoc Cancer Res"},{"key":"2026102103474834200_bpag025-B15","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.jphotobiol.2017.03.024","article-title":"Fluorescence properties of doxorubicin in PBS buffer and PVA films","volume":"170","author":"Shah","year":"2017","journal-title":"J Photochem Photobiol B"},{"key":"2026102103474834200_bpag025-B16","doi-asserted-by":"publisher","first-page":"432","DOI":"10.20455\/ros.2016.873","article-title":"Fluorescence-based assays for measuring doxorubicin in biological systems","volume":"2","author":"Kauffman","year":"2016","journal-title":"React Oxyg Species (Apex, NC)"},{"key":"2026102103474834200_bpag025-B17","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1007\/s00418-020-01857-x","article-title":"Detection of doxorubicin, cisplatin and therapeutic antibodies in formalin-fixed paraffin-embedded human cancer cells","volume":"153","author":"B\u00f6ckelmann","year":"2020","journal-title":"Histochem Cell Biol"},{"key":"2026102103474834200_bpag025-B18","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1016\/j.biomaterials.2011.10.043","article-title":"In vivo treatment of tumors using host-guest conjugated nanoparticles functionalized with doxorubicin and therapeutic gene pTRAIL","volume":"33","author":"Fan","year":"2012","journal-title":"Biomaterials"},{"key":"2026102103474834200_bpag025-B19","doi-asserted-by":"publisher","first-page":"8723","DOI":"10.1016\/j.biomaterials.2014.06.045","article-title":"PLK1shRNA and doxorubicin co-loaded thermosensitive PLGA-PEG-PLGA hydrogels for osteosarcoma treatment","volume":"35","author":"Ma","year":"2014","journal-title":"Biomaterials"},{"key":"2026102103474834200_bpag025-B20","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1038\/nm.4087","article-title":"Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the predilection of breast cancer patients to doxorubicin-induced cardiotoxicity","volume":"22","author":"Burridge","year":"2016","journal-title":"Nat Med"},{"key":"2026102103474834200_bpag025-B21","doi-asserted-by":"publisher","first-page":"3176","DOI":"10.7150\/thno.23207","article-title":"The HER2 inhibitor lapatinib potentiates doxorubicin-induced cardiotoxicity through iNOS signaling","volume":"8","author":"Hsu","year":"2018","journal-title":"Theranostics"},{"key":"2026102103474834200_bpag025-B22","doi-asserted-by":"publisher","first-page":"e2000387","DOI":"10.1002\/adhm.202000387","article-title":"TME-responsive polyprodrug micelles for multistage delivery of doxorubicin with \u0131mproved cancer therapeutic efficacy in rodents","volume":"9","author":"Zhang","year":"2020","journal-title":"Adv Healthcare Mater"},{"key":"2026102103474834200_bpag025-B23","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1002\/0471142956.cy0941s65","article-title":"Kinetic viability assays using DRAQ7 probe","volume":"65","author":"Wlodkowic","year":"2013","journal-title":"Curr Protoc Cytom"},{"key":"2026102103474834200_bpag025-B24","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1002\/cyto.a.22228","article-title":"Real-time cell viability assays using a new anthracycline derivative DRAQ7\u00ae","volume":"83","author":"Akagi","year":"2013","journal-title":"Cytometry A"},{"key":"2026102103474834200_bpag025-B25","doi-asserted-by":"publisher","first-page":"5427","DOI":"10.21873\/anticanres.14553","article-title":"DRAQ7 as an alternative to MTT assay for measuring viability of glioma cells treated with polyphenols","volume":"40","author":"Rooprai","year":"2020","journal-title":"Anticancer Res"},{"key":"2026102103474834200_bpag025-B26","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1002\/cyto.a.22874","article-title":"Quantification methods for human and large animal leukocytes using DNA dyes by flow cytometry","volume":"89","author":"Pieper","year":"2016","journal-title":"Cytometry A"}],"container-title":["Biology Methods and Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/biomethods\/advance-article-pdf\/doi\/10.1093\/biomethods\/bpag025\/68363698\/bpag025.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biomethods\/article-pdf\/11\/1\/bpag025\/68363698\/bpag025.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/biomethods\/article-pdf\/11\/1\/bpag025\/68363698\/bpag025.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T07:48:01Z","timestamp":1792568881000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/biomethods\/article\/doi\/10.1093\/biomethods\/bpag025\/8690162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,12]]}},"URL":"https:\/\/doi.org\/10.1093\/biomethods\/bpag025","relation":{},"ISSN":["2396-8923"],"issn-type":[{"value":"2396-8923","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"bpag025"}}