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In this study, we developed a diffracted X-ray blinking (DXB) method to evaluate amyloid-\u03b2 (A\u03b2)-associated nanoscale fluctuations in fixed and permeabilized whole-mount Caenorhabditis elegans. Intracellular A\u03b2 expressed in neurons (nA\u03b2) or body-wall muscle cells (mA\u03b2) was labelled with gold nanoparticles using an antibody-mediated strategy, without dissection or sectioning. The labelled worms produced detectable Au(111) diffraction signals, and the resulting time-resolved diffraction intensity fluctuations were analysed by autocorrelation function (ACF) analysis. A comparison of the ACF decay constants obtained from nA\u03b2 and mA\u03b2 worms revealed that compared with nA\u03b2, mA\u03b2 contained a greater fraction of fast relaxation components. Furthermore, in-vitro DXB measurements of A\u03b2 revealed local fast relaxation components that changed over time in association with changes in A\u03b2 assemblies, partly resembling the A\u03b2-associated motional features observed in worms. These findings suggest that the detected relaxation components may reflect structurally diverse A\u03b2 states arising during aggregation. These results demonstrate the feasibility of applying DXB to detect A\u03b2-associated nanoscale fluctuation signatures in fixed whole-mount C. elegans and provide a methodological framework for analysing protein-associated dynamic states in animal tissue samples.<\/jats:p>","DOI":"10.1093\/biomethods\/bpag044","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T11:48:36Z","timestamp":1786535316000},"source":"Crossref","is-referenced-by-count":0,"title":["Whole-mount diffracted X-ray blinking analysis of intracellular amyloid-\u03b2-associated nanoscale dynamics in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1093","volume":"11","author":[{"given":"Kotaro","family":"Ozaki","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Ibaraki University , Hitachi 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