{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:03:23Z","timestamp":1792785803207,"version":"4.5.0"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":239,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Indexed Publication (PUTI) Q1 Research","award":["NKB-308\/UN2.RST\/HKP.05.00\/2024"],"award-info":[{"award-number":["NKB-308\/UN2.RST\/HKP.05.00\/2024"]}]},{"name":"Faculty of Medicine, Universitas Indonesia, and Oxford University Clinical Research Unit","id":[{"id":"https:\/\/ror.org\/0116zj450","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000769","name":"University of Oxford","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Heinz bodies occur in patients experiencing specific drug-induced acute haemolytic crises, most often in those with an inherited deficiency in glucose-6-phosphate dehydrogenase activity. Laboratory methods for quantifying Heinz bodies in in vitro systems include direct microscopic counts from crystal violet-stained thin blood films or spectrophotometric measurement of free crystal violet in RBC suspensions treated for Heinz body induction. Each method is relatively laborious and prone to errors. In this study, we sought to evaluate and validate an improved flow cytometry-based method for detecting Heinz bodies in human red blood cells after in vitro exposure to phenylhydrazine using washed RBCs. We report good concordance between flow cytometric counts of Heinz bodies and microscopic and spectrophotometric analyses of the same suspensions. Flow cytometry offers a less laborious, more precise, and more accurate quantification of Heinz body formation, with strong potential to improve the ease of use in experimental applications.<\/jats:p>","DOI":"10.1093\/biomethods\/bpag047","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:11:25Z","timestamp":1787926285000},"source":"Crossref","is-referenced-by-count":0,"title":["Quantifying phenylhydrazine-induced Heinz body formation in normal human erythrocytes by flow cytometry versus microscopy or spectrophotometry"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7441-0422","authenticated-orcid":false,"given":"Mewahyu","family":"Dewi","sequence":"first","affiliation":[{"name":"Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia , Jakarta 10430,","place":["Indonesia"]},{"name":"Oxford University Clinical Research Unit Indonesia , Jakarta 10430,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sri Widia Azraki","family":"Jusman","sequence":"additional","affiliation":[{"name":"Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia , Jakarta 10430,","place":["Indonesia"]},{"name":"Department of Biochemistry and Molecular Biology, Universitas Indonesia , Jakarta 10430,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alida Roswita","family":"Harahap","sequence":"additional","affiliation":[{"name":"Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia , Jakarta 10430,","place":["Indonesia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Kevin","family":"Baird","sequence":"additional","affiliation":[{"name":"Oxford University Clinical Research Unit Indonesia , Jakarta 10430,","place":["Indonesia"]},{"name":"Nuffield Department of Medicine, Centre for Tropical Medicine & Global Health, University of Oxford , Oxford, OX3 7LF,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"key":"2026102103463073800_bpag047-B1","doi-asserted-by":"crossref","first-page":"e1001339","DOI":"10.1371\/journal.pmed.1001339","article-title":"G6PD deficiency prevalence and estimates of affected populations in malaria endemic countries: a geostatistical model-based map","volume":"9","author":"Howes","year":"2012","journal-title":"PLoS Med"},{"key":"2026102103463073800_bpag047-B2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1186\/s12936-018-2248-y","article-title":"Primaquine-induced haemolysis in females heterozygous for G6PD deficiency","volume":"17","author":"Chu","year":"2018","journal-title":"Malar J"},{"key":"2026102103463073800_bpag047-B3","first-page":"taz023","article-title":"Tafenoquine and G6PD: a primer for 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