{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:49:17Z","timestamp":1789184957938,"version":"build-2803163510"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T00:00:00Z","timestamp":1667606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Keloids represent one extreme of aberrant dermal wound healing and are characterized by fibroblast hyperproliferation and excessive deposition of extracellular matrix. Genetics is a major factor for predisposition to keloids and genome-wide association study has identified a single-nucleotide polymorphism (SNP) rs873549 at 1q41 as a susceptibility locus. The SNP rs873549, and the SNPs in strong linkage disequilibrium (LD) with rs873549, may be involved in keloid development. However, the functional significance of these SNPs in keloid pathogenesis remains elusive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To investigate the function and mechanism of SNP rs873549 and the SNPs in strong LD with rs873549 in keloids.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>SNPs in strong LD with rs873549 were analysed using Haploview. The expression levels of the genes near the susceptibility locus were analysed using quantitative real-time polymerase chain reaction. The interaction between rs1348270-containing enhancer and the long noncoding RNA down expressed in keloids (DEIK) (formerly RP11-400N13.1) promoter in fibroblasts was investigated using chromosome conformation capture. The enhancer activity of the rs1348270 locus was evaluated using luciferase reporter assay. Knockdown experiments were used to explore the function of DEIK in keloids. RNA-Seq was performed to investigate the mechanism by which DEIK regulates the expression of collagens POSTN and COMP.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>rs1348270, an enhancer-located SNP in strong LD with rs873549, mediated looping with the promoter of DEIK. The risk variant was associated with decreased enhancer\u2013promoter interaction and DEIK down-expression in keloids. Mechanistically, downregulation of DEIK increased the expression of collagens POSTN and COMP through upregulating BMP2. Furthermore, correlation analysis revealed that DEIK expression was inversely correlated with BMP2, POSTN and COMP expression in both keloid and normal fibroblasts.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings suggest that the risk variant rs1348270 is located in an enhancer and is associated with the downregulation of DEIK in keloids, and that downregulation of DEIK increases the expression of collagens POSTN and COMP through BMP2 in keloid fibroblasts. These findings will help to provide a more thorough understanding of the role played by genetic factors in keloid development and may lead to new strategies for screening and therapy in keloid-susceptible populations.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bjd\/ljac025","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T14:18:13Z","timestamp":1674224293000},"page":"84-93","source":"Crossref","is-referenced-by-count":15,"title":["Risk single-nucleotide polymorphism-mediated enhancer\u2013promoter interaction drives keloids through long noncoding RNA down expressed in keloids"],"prefix":"10.1093","volume":"188","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9616-1607","authenticated-orcid":false,"given":"Cheng-Cheng","family":"Deng","sequence":"first","affiliation":[{"name":"Dermatology Hospital, Southern Medical University , Guangzhou , 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School of Basic Medical Sciences, Southern Medical University , Guangzhou , China"},{"name":"State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Key Laboratory of Organ Failure Research (Ministry of Education), Southern Medical University , Guangzhou , China"},{"name":"Department of Nephrology, Shenzhen Hospital, Southern Medical University , Shenzhen , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1699-7074","authenticated-orcid":false,"given":"Zhili","family":"Rong","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University , Guangzhou , China"},{"name":"Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University , Guangzhou , China"},{"name":"State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Key Laboratory of Organ Failure Research (Ministry of Education), Southern Medical University , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7155-6079","authenticated-orcid":false,"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,5]]},"reference":[{"key":"2023100516254935200_ljac025-B1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.matbio.2016.01.013","article-title":"Keloids: the paradigm of skin fibrosis \u2013 pathomechanisms and treatment","volume":"51","author":"Andrews","year":"2016","journal-title":"Matrix Biol"},{"key":"2023100516254935200_ljac025-B2","doi-asserted-by":"crossref","first-page":"360","DOI":"10.3389\/fcell.2020.00360","article-title":"The keloid disorder: heterogeneity, histopathology, mechanisms and 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