{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:23:23Z","timestamp":1792794203350,"version":"4.5.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T00:00:00Z","timestamp":1685145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001602","name":"Science Foundation Ireland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"crossref"}]},{"name":"City of Dublin Skin and Cancer Hospital Charity","id":[{"id":"https:\/\/ror.org\/055n6t614","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Wellcome Trust and the Health Research Board","id":[{"id":"https:\/\/ror.org\/003hb2249","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Treatment for the debilitating disease hidradenitis suppurativa (HS) is inadequate in many patients. Despite an incidence of approximately 1%, HS is often under-recognized and underdiagnosed, and is associated with a high morbidity and poor quality of life.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To gain a better understanding of the pathogenesis of HS, in order to design new therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We employed single-cell RNA sequencing to analyse gene expression in immune cells isolated from involved HS skin vs. healthy skin. Flow cytometry was used to quantify the absolute numbers of the main immune populations. The secretion of inflammatory mediators from skin explant cultures was measured using multiplex and enzyme-linked immunosorbent assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Single-cell RNA sequencing analysis identified a significant enrichment in the frequency of plasma cells, T helper (Th) 17 cells and dendritic cell subsets in HS skin, and the immune transcriptome was distinct and more heterogeneous than healthy skin. Flow cytometry revealed significantly increased numbers of T cells, B cells, neutrophils, dermal macrophages and dendritic cells in HS skin. Genes and pathways associated with Th17 cells, interleukin (IL)-17, IL-1\u03b2 and the NLRP3 inflammasome were enhanced in HS skin, particularly in samples with a high inflammatory load. Inflammasome constituent genes principally mapped to Langerhans cells and a subpopulation of dendritic cells. The secretome of HS skin explants contained significantly increased concentrations of inflammatory mediators, including IL-1\u03b2 and IL-17A, and culture with an NLRP3 inflammasome inhibitor significantly reduced the secretion of these, as well as other, key mediators of inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data provide a rationale for targeting the NLRP3 inflammasome in HS using small-molecule inhibitors that are currently being tested for other indications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bjd\/ljad184","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T07:14:00Z","timestamp":1685171640000},"page":"447-458","source":"Crossref","is-referenced-by-count":39,"title":["Targeting the NLRP3 inflammasome reduces inflammation in hidradenitis suppurativa skin"],"prefix":"10.1093","volume":"189","author":[{"given":"Barry","family":"Moran","sequence":"first","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin , Dublin , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1701-1665","authenticated-orcid":false,"given":"Conor M","family":"Smith","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin , Dublin , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Zaborowski","sequence":"additional","affiliation":[{"name":"Department of Surgery, St. Vincent\u2019s University Hospital , Dublin , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Ryan","sequence":"additional","affiliation":[{"name":"AbbVie, Immunology Discovery Research, AbbVie Bioresearch Center , Worcester, MA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozsef","family":"Karman","sequence":"additional","affiliation":[{"name":"AbbVie, Immunology Systems Computational Biology, Cambridge Research Center , Cambridge, MA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert\u00a0W","family":"Dunstan","sequence":"additional","affiliation":[{"name":"AbbVie, Immunology Discovery Research, AbbVie Bioresearch Center , Worcester, MA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M","family":"Smith","sequence":"additional","affiliation":[{"name":"AbbVie, Immunology Systems Computational Biology, Cambridge Research Center , Cambridge, MA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roisin","family":"Hambly","sequence":"additional","affiliation":[{"name":"Department of Dermatology, St. Vincent\u2019s University Hospital and Charles Institute of Dermatology, University College Dublin , Dublin,\u00a0 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Musilova","sequence":"additional","affiliation":[{"name":"Education and Research Centre, University College Dublin , Dublin , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0420-5252","authenticated-orcid":false,"given":"Andreea\u00a0","family":"Petrasca","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin , Dublin , Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelie","family":"Fabre","sequence":"additional","affiliation":[{"name":"Department of Histopathology, St. Vincent\u2019s University Hospital and School of Medicine, University College Dublin , 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