{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:28:02Z","timestamp":1758475682217,"version":"3.37.3"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011440","name":"was","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["JP21bk0104083"],"award-info":[{"award-number":["JP21bk0104083"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010677","name":"Health","doi-asserted-by":"publisher","award":["23FC1039"],"award-info":[{"award-number":["23FC1039"]}],"id":[{"id":"10.13039\/100010677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000725","name":"the","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JSPS","doi-asserted-by":"publisher","award":["JP21H02941"],"award-info":[{"award-number":["JP21H02941"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"JST","doi-asserted-by":"publisher","award":["JPMJFR210R"],"award-info":[{"award-number":["JPMJFR210R"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>No efficient treatment has yet been established for epidermolytic ichthyosis (EI), which is caused by pathogenic variants of KRT1 or KRT10. Patients with ichthyosis with confetti (IWC) have multiple normal-appearing spots, caused by the revertant somatic recombination of pathogenic variants that occurs at each spot independently. Additionally, some patients with EI have large areas of normal skin due to revertant postzygotic mosaicism.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To assess the feasibility of transplanting cultured epidermal autografts (CEAs) produced from revertant epidermal keratinocytes in patients with EI and IWC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We performed a clinical trial of treatment with CEAs produced from each patient\u2019s own revertant epidermal keratinocytes as a proof-of-concept study. This was a single-arm, open, unmasked, uncontrolled, single-assignment, treatment-purpose study. The primary outcome was the percentage area that lacked recurrence of ichthyosis lesions 4 weeks after the final transplant. The secondary outcome was the percentage area lacking recurrence of ichthyosis lesions 24 weeks after the initial transplantation. The trial was registered with the Japan Registry of Clinical Trials (jRCTb041190097).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We successfully produced CEAs from genetically confirmed revertant skin from two patients with mosaic EI and from one patient with IWC and confirmed by amplicon sequencing and droplet digital polymerase chain reaction analysis that the CEAs mainly consisted of revertant wild-type cells. Single-cell RNA sequencing analysis confirmed the normal proliferation and safety profiling of CEAs. CEAs were transplanted onto desquamated lesional sites in the patients. Four weeks post-transplantation, the percentage area lacking recurrence of ichthyosis lesions in the three patients was 40%, 100% and 100% respectively, although recurrence of ichthyosis lesions was seen at the site of CEA transplantation in all three patients at 24 weeks post-transplantation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CEAs from normal skin have the potential to be a safe and local treatment option for EI and IWC.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bjd\/ljae193","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T12:41:48Z","timestamp":1715344908000},"page":"397-404","source":"Crossref","is-referenced-by-count":5,"title":["Treating epidermolytic ichthyosis and ichthyosis with confetti with epidermal autografts cultured from revertant\u00a0skin"],"prefix":"10.1093","volume":"191","author":[{"given":"Kana","family":"Tanahashi","sequence":"first","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiro","family":"Kono","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"},{"name":"Department of Dermatology and Plastic Surgery, Akita University Graduate School of Medicine , Akita , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takenori","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4049-7782","authenticated-orcid":false,"given":"Yuika","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masukazu","family":"Inoie","sequence":"additional","affiliation":[{"name":"Japan Tissue Engineering Co., Ltd. , Gamagori , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yachiyo","family":"Kuwatsuka","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Nagoya University Hospital , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumie","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Nagoya University Hospital , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5958-2875","authenticated-orcid":false,"given":"Takuya","family":"Takeichi","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"},{"name":"Nagoya University Institute for Advanced Research , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5863-9315","authenticated-orcid":false,"given":"Masashi","family":"Akiyama","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Nagoya University Graduate School of Medicine , Nagoya , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"2024081418365364300_ljae193-B1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.jaad.2009.11.020","article-title":"Revised nomenclature 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