{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:56:25Z","timestamp":1790855785848,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Christian\u2013Albrechts University of Kiel"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Mycosis fungoides (MF) is a rare malignancy that is characterized by the presence of circulating tumour cells (CTCs) in a subgroup of patients. Reliably distinguishing MF from inflammatory skin conditions is challenging.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To evaluate the potential benefits of next-generation sequencing (NGS)-based T-cell receptor rearrangement repertoire analysis in detecting clonal rearrangements in MF and inflammatory skin conditions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Skin biopsies and blood samples from 33 patients with MF and 10 patients with inflammatory skin conditions were analysed using TRB and TRG NGS. Twenty-seven patients had early-stage IA (n = 19) and IB (n = 8) MF, and six had advanced-stage disease (IIB, n = 5; IIIA, n = 1).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Analysis applying standard abundance thresholds identified at least one clonal rearrangement in the skin DNA of 97% (n = 32\/33) of patients with MF and in 90% (n = 9\/10) of those with inflammatory skin conditions. To enhance specificity, an abundance and distribution-based approach was applied, which considered only rearrangements that significantly stood out from the physiological background as clonal (MF, n = 29\/33; inflammatory skin conditions, n = 1\/10), allowing for highly sensitive (88%) and specific (90%) discrimination between MF and other inflammatory skin conditions. CTCs were detected in 46% (n = 11\/24) of patients with early-stage MF and in 60% (n = 3\/5) of those with late-stage MF.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>NGS-based T-cell receptor repertoire analysis is a highly sensitive and specific method for the differential diagnosis of early-stage MF vs. inflammatory skin conditions, and for the sensitive molecular detection of CTCs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bjd\/ljae425","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T19:17:21Z","timestamp":1730315841000},"page":"492-500","source":"Crossref","is-referenced-by-count":8,"title":["Mycosis fungoides: differentiation from inflammation and detection of circulating tumour cells with the EuroClonality next-generation sequencing assay"],"prefix":"10.1093","volume":"192","author":[{"given":"Ulrike","family":"Wehkamp","sequence":"first","affiliation":[{"name":"Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]},{"name":"Medical School Hamburg , Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Pietzka","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]},{"name":"Department of Hematology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Kotrov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Jost","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilske","family":"Oschlies","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hematopathology Section, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agatha","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel , Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Baldus","sequence":"additional","affiliation":[{"name":"Department of Hematology, University Hospital Schleswig-Holstein, Campus 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