{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T02:57:45Z","timestamp":1770346665498,"version":"3.49.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:00:00Z","timestamp":1758326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473508"],"award-info":[{"award-number":["82473508"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173396"],"award-info":[{"award-number":["82173396"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation of Shanghai","award":["23ZR1456100"],"award-info":[{"award-number":["23ZR1456100"]}]},{"name":"Shanghai Skin Disease Hospital Clinical Research Incubation","award":["20240801103"],"award-info":[{"award-number":["20240801103"]}]},{"name":"Clinical Research Plan of SHDC","award":["SHDC22022302"],"award-info":[{"award-number":["SHDC22022302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The pathogenesis of large and giant congenital melanocytic naevi (L\/GCMN) remains poorly understood, presenting significant challenges for its treatment. To date, no treatment guidelines for L\/GCMN have been established. Moreover, single-cell technologies have not been applied in L\/GCMN research, impeding an understanding of the disease at the cellular level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To elaborate on the transcriptomic landscape of cells in the skin microenvironment of L\/GCMN, explore the crucial molecular mechanisms of melanocyte pathological changes and investigate their interactions with other cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used a combination of single-cell and bulk RNA sequencing of lesional and nonlesional skin samples from patients with L\/GCMN and healthy skin samples. Moreover, we also conducted Western blotting, quantitative reverse transcription polymerase chain reaction, and immunohistochemical and multiplex immunofluorescence to validate our results. Additionally, we isolated primary naevomelanocytes and explored the impact of T-box transcription factor 2 (TBX2) on the biologic functions of naevomelanocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Patients with L\/GCMN have a higher density of melanocytes and enhanced functions in melanin synthesis-related processes. TBX2 serves as a core transcription factor for a melanocyte subpopulation that is specifically expressed in GCMN. It is closely associated with the proliferation and melanin synthesis of naevomelanocytes. Additionally, abnormal activation of the pleiotrophin\u2013protein tyrosine phosphatase receptor type Z1 and insulin-like growth factor 1 (IGF-1)\u2013IGF-1 receptor signalling pathways was detected in L\/GCMN melanocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The study provides initial insights into the transcriptional landscape and cellular interaction networks of L\/GCMN, laying a foundation for further exploration of its molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bjd\/ljaf360","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T11:29:52Z","timestamp":1757417392000},"page":"99-110","source":"Crossref","is-referenced-by-count":1,"title":["Single-cell RNA sequencing reveals the transcriptomic landscape of and potential targets for large and giant congenital melanocytic naevi"],"prefix":"10.1093","volume":"194","author":[{"given":"Wei","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]},{"name":"Department of Dermatology, Shanghai Children\u2019s Medical Center, School of Medicine, Shanghai Jiao Tong University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1728-0769","authenticated-orcid":false,"given":"Mingyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiashe","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongyi","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3758-1390","authenticated-orcid":false,"given":"Yeqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Skin Disease Hospital, School of Medicine, Tongji University , Shanghai 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