{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:57:07Z","timestamp":1779307027067,"version":"3.51.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T00:00:00Z","timestamp":1683936000000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>New generation ultra-fast fluorescence confocal microscopy allows the ex vivo intraoperative analysis of fresh tissue. The High resolution Imaging for Breast carcInoma detection in ex vivo Specimens after breast Conserving sUrgery by hiStolog Scanner (HIBISCUSS) project aimed to develop an online learning program to recognize the main breast tissue features on ultra-fast fluorescence confocal microscopy images and to evaluate the performance of surgeons and pathologists in diagnosing cancerous and non-cancerous breast tissue in ultra-fast fluorescence confocal microscopy images.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Patients who underwent conservative surgery or mastectomy for breast carcinoma (invasive or in situ lesions) were included. The fresh specimens were stained with a fluorescent dye and imaged using a large field-of-view (20\u2005cm2) ultra-fast fluorescence confocal microscope.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>One hundred and eighty-one patients were included. The images from 55 patients were annotated to generate learning sheets and images from 126 patients were blindly interpreted by seven surgeons and two pathologists. The time for tissue processing and ultra-fast fluorescence confocal microscopy imaging was between 8 and 10\u2005min. The training program was composed of 110 images divided into nine learning sessions. The final database for blind performance assessment comprised 300 images. The mean duration for one training session and one performance round was 17 and 27\u2005min respectively. The performance of pathologists was almost perfect with 99.6 per cent (standard deviation (s.d.) 5.4 per cent) accuracy. Surgeons\u2019 accuracy significantly increased (P = 0.001) from 83 per cent (s.d. 8.4 per cent) in round 1 to 98 per cent (s.d. 4.1 per cent) in round 7 as well as the sensitivity (P = 0.004). Specificity increased without significance from 84 per cent (s.d. 16.7 per cent) in round 1 to 87 per cent (s.d. 16.4 per cent) in round 7 (P = 0.060).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Pathologists and surgeons showed a short learning curve in differentiating breast cancer from non-cancerous tissue in ultra-fast fluorescence confocal microscopy images. Performance assessment for both specialties supports ultra-fast fluorescence confocal microscopy evaluation for intraoperative management.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Registration number<\/jats:title>\n                  <jats:p>NCT04976556 (http:\/\/www.clinicaltrials.gov).<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bjsopen\/zrad046","type":"journal-article","created":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T12:21:41Z","timestamp":1683980501000},"source":"Crossref","is-referenced-by-count":22,"title":["Breast carcinoma detection in <i>ex vivo<\/i> fresh human breast surgical specimens using a fast slide-free confocal microscopy scanner: HIBISCUSS project"],"prefix":"10.1093","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-1720","authenticated-orcid":false,"given":"Angelica","family":"Conversano","sequence":"first","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de Chirurgie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-8868","authenticated-orcid":false,"given":"Muriel","family":"Abbaci","sequence":"additional","affiliation":[{"name":"Gustave Roussy, Plate-forme Imagerie et Cytom\u00e9trie, UMS 23\/3655, Universit\u00e9 Paris-Saclay , Villejuif , France"},{"name":"Universit\u00e9 Paris-Saclay, CEA, CNRS, Inserm, Laboratoire d'Imagerie Biom\u00e9dicale Multimodale Paris Saclay , Orsay , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"van Diest","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Medical Center Utrecht , Utrecht , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lie","family":"Roulot","sequence":"additional","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de Chirurgie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Falco","sequence":"additional","affiliation":[{"name":"Department of Oncology and Advanced Technologies, Breast Surgery Unit, IRCSS Santa Maria Nuova Hospital , Reggio Emilia , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malek","family":"Ferchiou","sequence":"additional","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de pathologie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saverio","family":"Coiro","sequence":"additional","affiliation":[{"name":"Department of Oncology and Advanced Technologies, Breast Surgery Unit, IRCSS Santa Maria Nuova Hospital , Reggio Emilia , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Richir","sequence":"additional","affiliation":[{"name":"Department of Surgery, University Medical Centre Utrecht , Utrecht, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre-Michel","family":"Genolet","sequence":"additional","affiliation":[{"name":"Espacegyneco , Pully , Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carine","family":"Clement","sequence":"additional","affiliation":[{"name":"Espacegyneco , Pully , Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odile","family":"Casiraghi","sequence":"additional","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de pathologie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aicha Ben","family":"Lahkdar","sequence":"additional","affiliation":[{"name":"SCM Bichat , Paris , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nizard","family":"Labaied","sequence":"additional","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de pathologie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moira","family":"Ragazzi","sequence":"additional","affiliation":[{"name":"Pathology Unit, Azienda USL-IRCCS di Reggio Emilia , Reggio Emilia , Italy"},{"name":"Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia , Modena , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Christine","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Gustave Roussy, D\u00e9partement de pathologie, Universit\u00e9 Paris-Saclay , Villejuif , France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,5,13]]},"container-title":["BJS Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bjsopen\/article-pdf\/7\/3\/zrad046\/50307092\/zrad046.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bjsopen\/article-pdf\/7\/3\/zrad046\/50307092\/zrad046.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T12:21:42Z","timestamp":1683980502000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bjsopen\/article\/doi\/10.1093\/bjsopen\/zrad046\/7161299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,5,5]]}},"URL":"https:\/\/doi.org\/10.1093\/bjsopen\/zrad046","relation":{},"ISSN":["2474-9842"],"issn-type":[{"value":"2474-9842","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,6]]},"published":{"date-parts":[[2023,5,5]]},"article-number":"zrad046"}}