{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:36:12Z","timestamp":1792614972641,"version":"4.5.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T00:00:00Z","timestamp":1725408000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","award":["2023_EKMS.13"],"award-info":[{"award-number":["2023_EKMS.13"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC-CoG 819933"],"award-info":[{"award-number":["ERC-CoG 819933"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012331","name":"LEO Foundation","doi-asserted-by":"publisher","award":["LF-OC-21-000835"],"award-info":[{"award-number":["LF-OC-21-000835"]}],"id":[{"id":"10.13039\/501100012331","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001648","name":"European Foundation for the Study of Diabetes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001648","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Anastomotic leakage following colorectal surgery remains a significant complication despite advances in surgical techniques. Recent findings on serosal injury repair in coelomic cavities, such as the peritoneum, challenge the current understanding of the cellular origins and mechanisms underlying intestinal anastomotic healing. Understanding the contribution of each layer of the intestinal wall during anastomotic healing is needed to find new therapeutic strategies to prevent anastomotic leakage. The aim of this experimental study was to investigate the role of the serosal layer of the intestinal wall in anastomotic healing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and methods<\/jats:title>\n                  <jats:p>Comprehensive histologic analysis of human and murine anastomoses was performed to elucidate histologic changes in the different intestinal layers during anastomotic healing. In vivo staining of the extracellular matrix (ECM) in the serosal layer was performed using a fluorophore-conjugated N-hydroxysuccinimide-ester before anastomosis surgery in a murine model.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Histological examination of both human and murine anastomoses revealed that closure of the serosal layer occurred first during the healing process. In vivo serosal ECM staining demonstrated that a significant portion of the newly formed ECM within the anastomosis was indeed deposited onto the serosal layer. Furthermore, mesenchymal cells within the anastomotic scar were positive for mesothelial cell markers, podoplanin and Wilms tumour protein.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In this experimental study, the results suggest that serosal scar formation is an important mechanism for anastomotic integrity in intestinal anastomoses. Mesothelial cells may significantly contribute to scar formation during anastomotic healing through epithelial-to-mesenchymal transition, potentially suggesting a novel therapeutic target to prevent anastomotic leakage by enhancing physiological healing processes.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bjsopen\/zrae108","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T08:01:54Z","timestamp":1725523314000},"source":"Crossref","is-referenced-by-count":10,"title":["Role of the serosa in intestinal anastomotic healing: insights from in-depth histological analysis of human and murine anastomoses"],"prefix":"10.1093","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3188-2160","authenticated-orcid":false,"given":"Marie-Christin","family":"Weber","sequence":"first","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00e9","family":"Clees","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Buck","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]},{"name":"Institute for Advanced Study, Technical University of Munich , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Fischer","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Biology and Medicine, Helmholtz Munich , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella","family":"Steffani","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Wilhelm","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Martignoni","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Friess","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuval","family":"Rinkevich","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Biology and Medicine, Helmholtz Munich , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp-Alexander","family":"Neumann","sequence":"additional","affiliation":[{"name":"Department of Surgery, Technical University of Munich, TUM School of Medicine and Health , Munich ,","place":["Germany"]},{"name":"Institute for Advanced Study, Technical University of Munich , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,4]]},"reference":[{"key":"2024102920564985300_zrae108-B1","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1097\/SLA.0b013e3182128929","article-title":"Increased local recurrence and reduced survival 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