{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:39:41Z","timestamp":1787067581141,"version":"build-2736575974"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T00:00:00Z","timestamp":1735516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University College Hospital Charity","award":["172627)"],"award-info":[{"award-number":["172627)"]}]},{"name":"Egyptian missions\u2019 sector award","award":["MM42\/19"],"award-info":[{"award-number":["MM42\/19"]}]},{"DOI":"10.13039\/501100008721","name":"UCLH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008721","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Charity Grant","award":["534982"],"award-info":[{"award-number":["534982"]}]},{"name":"Charity Grant","award":["MM42\/19"],"award-info":[{"award-number":["MM42\/19"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>While most thyroid nodules are benign, 7\u201315% are malignant. Patients with indeterminate thyroid nodules (specifically Bethesda IV\/Thy3f) often undergo diagnostic hemithyroidectomy to reach a diagnosis on final histology. The aim of this study was to assess the feasibility of circulating large extracellular vesicles as diagnostic biomarkers in patients presenting with Thy3f thyroid nodules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a two-gate diagnostic accuracy study; patients with Thy3f thyroid nodules were age, sex and body mass index matched to healthy individuals. Final histology confirmed benign and malignant diagnoses. Plasma large extracellular vesicle counts were quantified using flow cytometry. Large extracellular vesicle microRNA and protein profiles were identified using next generation sequencing and mass spectrometry, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 42 patients with Thy3f nodules (22 with cancer, 20 with non-cancer diagnosis) and 16 healthy controls were included. Total large extracellular vesicle concentrations and the concentrations of extracellular vesicles expressing epithelial cell adhesion molecule and the cancer markers atypical chemokine receptor type 7, extracellular matrix metalloproteinase inducer and syndecan-4 were significantly higher in patients with Thy3f nodules (cancer and non-cancer) compared with healthy individuals. In patients with cancerous versus non-cancer Thy3f nodules, one microRNA was upregulated: mir-195\u20133p (P &amp;lt; 0.001). Five were downregulated: mir-3176 (P &amp;lt; 0.001), mir-205-5p (P &amp;lt; 0.001), novel-hsa-mir-208-3p (P &amp;lt; 0.001), mir-3529-3p (P = 0.01) and let-7i-3p (P = 0.02). Furthermore, three large extracellular vesicle proteins (kallikrein-related peptidase11 (KLK11) (P = 0.001), alpha-1-acid glycoprotein 2 (A1AG2) (P &amp;lt;0.001) and small integral membrane protein 1 (SMIM1) (P = 0.04)) were significantly upregulated, while 20 large extracellular vesicle proteins were significantly downregulated (most downregulated: chemokine (C-X-C motif) ligand 7 (CXCL7), tubulin beta chain 1 (TBB1), binding immunoglobulin protein (BIP) and actinin alpha 1 (ACTN1) (P &amp;lt; 0.001)) in cancerous compared with non-cancer Thy3f nodules.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Circulating large extracellular vesicle miRNA and protein profiles have a high diagnostic value to discriminate between benign and malignant nodules for patients with Thy3f cytology. Further validation for clinical performance will be needed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bjsopen\/zrae139","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:59:23Z","timestamp":1730249963000},"source":"Crossref","is-referenced-by-count":6,"title":["Circulating large extracellular vesicles as diagnostic biomarkers of indeterminate thyroid nodules: multi-platform omics analysis"],"prefix":"10.1093","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2849-9961","authenticated-orcid":false,"given":"Nada M","family":"Ahmed","sequence":"first","affiliation":[{"name":"Institute of Cardiovascular Sciences, University College London , London ,","place":["UK"]},{"name":"Pathology Department, Alexandria University , Alexandria ,","place":["Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4582-0788","authenticated-orcid":false,"given":"Mohammad M R","family":"Eddama","sequence":"additional","affiliation":[{"name":"Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5824-5009","authenticated-orcid":false,"given":"Kevin","family":"Beatson","sequence":"additional","affiliation":[{"name":"Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rijan","family":"Gurung","sequence":"additional","affiliation":[{"name":"Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jigisha","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Iskandar","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Perioperative Medicine, University College London Hospitals , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7990-6302","authenticated-orcid":false,"given":"Alaa","family":"Abdel-Salam","sequence":"additional","affiliation":[{"name":"Endocrine Surgery Unit, University College London Hospitals , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Al-Omar","sequence":"additional","affiliation":[{"name":"Endocrine Surgery Unit, University College London Hospitals , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7630-9985","authenticated-orcid":false,"given":"Tarek","family":"Abdel-Aziz","sequence":"additional","affiliation":[{"name":"Endocrine Surgery Unit, University College London Hospitals , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Clapp","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular Sciences, University College London , London ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,12,30]]},"reference":[{"key":"2025030423520380700_zrae139-B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/thy.2015.0020","article-title":"2015 American Thyroid Association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American Thyroid Association guidelines task force on thyroid nodules and differentiated thyroid cancer","volume":"26","author":"Haugen","year":"2016","journal-title":"Thyroid"},{"key":"2025030423520380700_zrae139-B2","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1089\/thy.2017.0500","article-title":"The 2017 Bethesda system for reporting thyroid cytopathology","volume":"27","author":"Cibas","year":"2017","journal-title":"Thyroid"},{"key":"2025030423520380700_zrae139-B3","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1002\/hed.25160","article-title":"Investigation of thyroid nodules: a practical algorithm and review of guidelines","volume":"40","author":"Song","year":"2018","journal-title":"Head Neck"},{"key":"2025030423520380700_zrae139-B4","author":"Cross","year":"2024"},{"key":"2025030423520380700_zrae139-B5","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1097\/SLA.0000000000003580","article-title":"The American Association of Endocrine Surgeons guidelines for the definitive surgical management of thyroid disease in adults","volume":"271","author":"Patel","year":"2020","journal-title":"Ann Surg"},{"key":"2025030423520380700_zrae139-B6","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1159\/000345498","article-title":"Hemithyroidectomy: a meta-analysis of postoperative need for hormone replacement and complications","volume":"75","author":"Kandil","year":"2013","journal-title":"ORL J Otorhinolaryngol Relat Spec"},{"key":"2025030423520380700_zrae139-B7","doi-asserted-by":"crossref","first-page":"204","DOI":"10.3390\/cancers14010204","article-title":"Emerging biomarkers in thyroid practice and research","volume":"14","author":"Agarwal","year":"2021","journal-title":"Cancers (Basel)"},{"key":"2025030423520380700_zrae139-B8","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.tips.2019.01.006","article-title":"Liquid biopsies in cancer diagnosis, monitoring, and prognosis","volume":"40","author":"De Rubis","year":"2019","journal-title":"Trends Pharmacol Sci"},{"key":"2025030423520380700_zrae139-B9","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0049-3848(03)00064-1","article-title":"Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis","volume":"109","author":"Jimenez","year":"2003","journal-title":"Thromb Res"},{"key":"2025030423520380700_zrae139-B10","doi-asserted-by":"crossref","first-page":"28414","DOI":"10.3402\/jev.v4.28414","article-title":"Circulating microparticles, protein C, free protein S and endothelial vascular markers in children with sickle cell anaemia","volume":"4","author":"Piccin","year":"2015","journal-title":"J Extracell Vesicles"},{"key":"2025030423520380700_zrae139-B11","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1002\/1878-0261.12777","article-title":"DNA in extracellular vesicles: biological and clinical aspects","volume":"15","author":"Elzanowska","year":"2020","journal-title":"Mol Oncol"},{"key":"2025030423520380700_zrae139-B12","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1159\/000491898","article-title":"Circulating tumor cells correlate with clinicopathological features and outcomes in differentiated thyroid cancer","volume":"48","author":"Qiu","year":"2018","journal-title":"Cell Physiol Biochem"},{"key":"2025030423520380700_zrae139-B13","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.mam.2017.11.009","article-title":"Extracellular vesicles compartment in liquid biopsies: clinical application","volume":"60","author":"Garcia-Romero","year":"2018","journal-title":"Mol Aspects Med"},{"key":"2025030423520380700_zrae139-B14","doi-asserted-by":"crossref","first-page":"e0136133","DOI":"10.1371\/journal.pone.0136133","article-title":"Characterization of RNA from exosomes and other extracellular vesicles isolated by a novel spin column-based method","volume":"10","author":"Enderle","year":"2015","journal-title":"PLoS One"},{"key":"2025030423520380700_zrae139-B15","volume-title":"Evolution of Translational Omics: Lessons Learned and the Path Forward","author":"Committee on the Review of Omics-Based Tests for Predicting Patient Outcomes in Clinical Trials, Board on Health Care Services, Board on Health Sciences Policy, Institute of Medicine","year":"2012"},{"key":"2025030423520380700_zrae139-B16","doi-asserted-by":"crossref","first-page":"e12385","DOI":"10.1002\/jev2.12385","article-title":"MIBlood-EV: minimal information to enhance the quality and reproducibility of blood extracellular vesicle research","volume":"12","author":"Lucien","year":"2023","journal-title":"J Extracell Vesicles"},{"key":"2025030423520380700_zrae139-B17","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1373\/clinchem.2017.278978","article-title":"Comparison of generic fluorescent markers for detection of extracellular vesicles by flow cytometry","volume":"64","author":"de Rond","year":"2018","journal-title":"Clin Chem"},{"key":"2025030423520380700_zrae139-B18","doi-asserted-by":"crossref","first-page":"1713526","DOI":"10.1080\/20013078.2020.1713526","article-title":"MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments","volume":"9","author":"Welsh","year":"2020","journal-title":"J Extracell Vesicles"},{"key":"2025030423520380700_zrae139-B19","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1101\/gr.229102","article-title":"The human genome browser at UCSC","volume":"12","author":"Kent","year":"2002","journal-title":"Genome Res"},{"key":"2025030423520380700_zrae139-B20","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nmeth.4185","article-title":"EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research","volume":"14","author":"Van Deun","year":"2017","journal-title":"Nat Methods"},{"key":"2025030423520380700_zrae139-B21","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat Methods"},{"key":"2025030423520380700_zrae139-B22","author":"Oliveros"},{"key":"2025030423520380700_zrae139-B23","doi-asserted-by":"crossref","first-page":"10851","DOI":"10.1038\/s41598-022-14211-z","article-title":"A comparative analysis of extracellular vesicles (EVs) from human and feline plasma","volume":"12","author":"Howard","year":"2022","journal-title":"Sci Rep"},{"key":"2025030423520380700_zrae139-B24","doi-asserted-by":"crossref","first-page":"D1694","DOI":"10.1093\/nar\/gkad1007","article-title":"Vesiclepedia 2024: an extracellular vesicles and extracellular particles repository","volume":"52","author":"Chitti","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"2025030423520380700_zrae139-B25","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1002\/cam4.4664","article-title":"The role of microvesicles as biomarkers in the screening of colorectal neoplasm","volume":"11","author":"Eddama","year":"2022","journal-title":"Cancer Med"},{"key":"2025030423520380700_zrae139-B26","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s12020-021-02847-2","article-title":"Analysis of serum microRNA in exosomal vehicles of papillary thyroid cancer","volume":"75","author":"Capriglione","year":"2022","journal-title":"Endocrine"},{"key":"2025030423520380700_zrae139-B27","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1007\/s12020-021-02949-x","article-title":"Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma","volume":"75","author":"Chen","year":"2022","journal-title":"Endocrine"},{"key":"2025030423520380700_zrae139-B28","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1530\/EJE-19-0524","article-title":"Identification of exosomal miRNA biomarkers for diagnosis of papillary thyroid cancer by small RNA sequencing","volume":"182","author":"Dai","year":"2019","journal-title":"Eur J Endocrinol"},{"key":"2025030423520380700_zrae139-B29","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1097\/CM9.0000000000001107","article-title":"A three-microRNA panel in serum as novel biomarker for papillary thyroid carcinoma diagnosis","volume":"133","author":"Zou","year":"2020","journal-title":"Chin Med J (Engl)"},{"key":"2025030423520380700_zrae139-B30","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1530\/ERC-21-0343","article-title":"Two miRNAs enriched in plasma extracellular vesicles are potential biomarkers for thyroid cancer","volume":"29","author":"Delcorte","year":"2022","journal-title":"Endocr Relat Cancer"},{"key":"2025030423520380700_zrae139-B31","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3390\/ijms25010071","article-title":"The use of tissue-on-chip technology to focus the search for extracellular vesicle miRNA biomarkers in thyroid disease","volume":"25","author":"Haigh","year":"2023","journal-title":"Int J Mol Sci"},{"key":"2025030423520380700_zrae139-B32","doi-asserted-by":"crossref","first-page":"516","DOI":"10.4149\/neo_2018_180824N643","article-title":"Exosomal miRNA423\u20135p mediated oncogene activity in papillary thyroid carcinoma: a potential diagnostic and biological target for cancer therapy","volume":"66","author":"Ye","year":"2019","journal-title":"Neoplasma"},{"key":"2025030423520380700_zrae139-B33","doi-asserted-by":"crossref","first-page":"149208","DOI":"10.1016\/j.bbrc.2023.149208","article-title":"Large extracellular vesicles transfer more prions and infect cell culture better than small extracellular vesicles","volume":"687","author":"Soukup","year":"2023","journal-title":"Biochem Biophys Res Commun"},{"key":"2025030423520380700_zrae139-B34","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1038\/s41416-024-02575-0","article-title":"Circulating small extracellular vesicle-based miRNA classifier for follicular thyroid carcinoma: a diagnostic study","volume":"130","author":"Li","year":"2024","journal-title":"Br J Cancer"},{"key":"2025030423520380700_zrae139-B35","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.3390\/cells9081917","article-title":"MiRNA let-7 from TPO(+) extracellular vesicles is a potential marker for a differential diagnosis of follicular thyroid nodules","volume":"9","author":"Zabegina","year":"2020","journal-title":"Cells"},{"key":"2025030423520380700_zrae139-B36","doi-asserted-by":"crossref","first-page":"eabh2169","DOI":"10.1126\/sciadv.abh2169","article-title":"A single-cell type transcriptomics map of human tissues","volume":"7","author":"Karlsson","year":"2021","journal-title":"Sci Adv"},{"key":"2025030423520380700_zrae139-B37","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s12902-020-0530-9","article-title":"Malignancy rates for Bethesda III and IV thyroid nodules: a retrospective study of the correlation between fine-needle aspiration cytology and histopathology","volume":"20","author":"Yaprak Bayrak","year":"2020","journal-title":"BMC Endocr Disord"},{"key":"2025030423520380700_zrae139-B38","doi-asserted-by":"crossref","first-page":"997","DOI":"10.17219\/acem\/160003","article-title":"Expression of micro-ribonucleic acids in thyroid nodules and serum to discriminate between follicular adenoma and cancer in patients with a fine needle aspiration biopsy classified as suspicious for follicular neoplasm: a preliminary study","volume":"32","author":"\u015awirta","year":"2023","journal-title":"Adv Clin Exp Med"},{"key":"2025030423520380700_zrae139-B39","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3390\/mps4010010","article-title":"Recent advances in miRNA delivery systems","volume":"4","author":"Dasgupta","year":"2021","journal-title":"Methods Protoc"},{"key":"2025030423520380700_zrae139-B40","doi-asserted-by":"crossref","first-page":"269","DOI":"10.2217\/fon.09.149","article-title":"Prognostic value and biological role of the kallikrein-related peptidases in human malignancies","volume":"6","author":"Mavridis","year":"2010","journal-title":"Future Oncol"},{"key":"2025030423520380700_zrae139-B41","doi-asserted-by":"publisher","DOI":"10.1007\/s10528\u2013024\u201310679\u20138","article-title":"KLK7, KLK10, and KLK11 in papillary thyroid cancer: bioinformatic analysis and experimental validation","author":"Ni","year":"2024;","journal-title":"Biochem Genet"},{"key":"2025030423520380700_zrae139-B42","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12953-023-00207-8","article-title":"Proteomics profiling for the global and acetylated proteins of papillary thyroid cancers","volume":"21","author":"Wei","year":"2023","journal-title":"Proteome Sci"},{"key":"2025030423520380700_zrae139-B43","doi-asserted-by":"crossref","first-page":"16106","DOI":"10.18632\/oncotarget.3867","article-title":"Orosomucoid 2 inhibits tumor metastasis and is upregulated by CCAAT\/enhancer binding protein \u03b2 in hepatocellular carcinomas","volume":"6","author":"Fang","year":"2015","journal-title":"Oncotarget"},{"key":"2025030423520380700_zrae139-B44","doi-asserted-by":"crossref","first-page":"e31868","DOI":"10.1371\/journal.pone.0031868","article-title":"The potential role of ORM2 in the development of colorectal cancer","volume":"7","author":"Zhang","year":"2012","journal-title":"PLoS One"},{"key":"2025030423520380700_zrae139-B45","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41419-021-03816-3","article-title":"Proteomics of extracellular vesicles in plasma reveals the characteristics and residual traces of COVID-19 patients without underlying diseases after 3 months of recovery","volume":"12","author":"Mao","year":"2021","journal-title":"Cell Death Dis"},{"key":"2025030423520380700_zrae139-B46","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.jmb.2015.09.019","article-title":"ExoCarta: a web-based compendium of exosomal cargo","volume":"428","author":"Keerthikumar","year":"2016","journal-title":"J Mol Biol"},{"key":"2025030423520380700_zrae139-B47","doi-asserted-by":"crossref","first-page":"BSR20200318","DOI":"10.1042\/BSR20200318","article-title":"SMIM1, carrier of the vel blood group, is a tail-anchored transmembrane protein and readily forms homodimers in a cell-free system","volume":"40","author":"Nylander","year":"2020","journal-title":"Biosci Rep"},{"key":"2025030423520380700_zrae139-B48","doi-asserted-by":"crossref","first-page":"86999","DOI":"10.18632\/oncotarget.13569","article-title":"Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers","volume":"7","author":"Hurwitz","year":"2016","journal-title":"Oncotarget"},{"key":"2025030423520380700_zrae139-B49","doi-asserted-by":"crossref","first-page":"e46822","DOI":"10.1371\/journal.pone.0046822","article-title":"Cause or effect of arteriogenesis: compositional alterations of microparticles from CAD patients undergoing external counterpulsation therapy","volume":"7","author":"Al Kaabi","year":"2012","journal-title":"PLoS One"},{"key":"2025030423520380700_zrae139-B50","doi-asserted-by":"crossref","first-page":"eaay5947","DOI":"10.1126\/science.aay5947","article-title":"An atlas of the protein-coding genes in the human, pig, and mouse brain","volume":"367","author":"Sj\u00f6stedt","year":"2020","journal-title":"Science"},{"key":"2025030423520380700_zrae139-B51","doi-asserted-by":"crossref","first-page":"286","DOI":"10.3390\/metabo10070286","article-title":"Find the needle in the haystack, then find it again: replication and validation in the \u2018omics era","volume":"10","author":"Perng","year":"2020","journal-title":"Metabolites"},{"key":"2025030423520380700_zrae139-B52","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/j.annonc.2021.05.806","article-title":"Clinical validation of a targeted methylation-based multi-cancer early detection test using an independent validation set","volume":"32","author":"Klein","year":"2021","journal-title":"Ann Oncol"},{"key":"2025030423520380700_zrae139-B53","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1210\/jc.2011-3059","article-title":"Circulating microRNA profiles as potential biomarkers for diagnosis of papillary thyroid carcinoma","volume":"97","author":"Yu","year":"2012","journal-title":"J Clin Endocrinol Metab"},{"key":"2025030423520380700_zrae139-B54","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1186\/s12967-023-03960-8","article-title":"Liquid biopsies: the future of cancer early detection","volume":"21","author":"Connal","year":"2023","journal-title":"J Transl Med"},{"key":"2025030423520380700_zrae139-B55","doi-asserted-by":"crossref","first-page":"83745","DOI":"10.18632\/oncotarget.19407","article-title":"Diagnostic potential of serum exosomal colorectal neoplasia differentially expressed long non-coding RNA (CRNDE-p) and microRNA-217 expression in colorectal carcinoma","volume":"8","author":"Yu","year":"2017","journal-title":"Oncotarget"},{"key":"2025030423520380700_zrae139-B56","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.eururo.2018.08.019","article-title":"A prospective adaptive utility trial to validate performance of a novel urine exosome gene expression assay to predict high-grade prostate cancer in patients with prostate-specific antigen 2\u201310\u2005ng\/ml at initial biopsy","volume":"74","author":"McKiernan","year":"2018","journal-title":"Eur Urol"},{"key":"2025030423520380700_zrae139-B57","doi-asserted-by":"crossref","first-page":"1113977","DOI":"10.3389\/fendo.2023.1113977","article-title":"Thyroid nodules: global, economic, and personal burdens","volume":"14","author":"Uppal","year":"2023","journal-title":"Front Endocrinol (Lausanne)"}],"container-title":["BJS Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bjsopen\/article-pdf\/9\/1\/zrae139\/61293757\/zrae139.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bjsopen\/article-pdf\/9\/1\/zrae139\/61293757\/zrae139.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T18:52:42Z","timestamp":1741114362000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bjsopen\/article\/doi\/10.1093\/bjsopen\/zrae139\/7934542"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,30]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,12,30]]}},"URL":"https:\/\/doi.org\/10.1093\/bjsopen\/zrae139","relation":{},"ISSN":["2474-9842"],"issn-type":[{"value":"2474-9842","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,2]]},"published":{"date-parts":[[2024,12,30]]},"article-number":"zrae139"}}