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However, later work disputed this by demonstrating the exquisite sensitivity of granulocytes to ex vivo isolation and labelling, and that excessive lung activity is artefactual.<\/jats:p><\/jats:sec><jats:sec><jats:title>Areas of agreement<\/jats:title><jats:p>Following refinement of labelling techniques, it was shown that the majority of marginated granulocytes are located in the spleen and bone marrow. The majority of leucocytes have a pulmonary vascular transit time only a few seconds longer than erythrocytes. The minority showing slow transit, ~5% in healthy persons, is increased in systemic inflammatory disorders that cause neutrophil priming and loss of deformability. Using a range of imaging techniques, including gamma camera imaging, whole-body counting and single photon-emission computerized tomography, labelled granulocytes were subsequently used to image pulmonary trafficking in lobar pneumonia, bronchiectasis, chronic obstructive pulmonary disease and adult respiratory distress syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Growing points<\/jats:title><jats:p>More recently, eosinophils have been separated in pure form using magnetic bead technology for the study of eosinophil trafficking in asthma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Areas timely for developing research<\/jats:title><jats:p>These include advancement of eosinophil imaging, development of monocyte labelling, development of cell labelling with PET tracers and the tracking of lymphocytes.<\/jats:p><\/jats:sec>","DOI":"10.1093\/bmb\/ldy022","type":"journal-article","created":{"date-parts":[[2018,7,21]],"date-time":"2018-07-21T04:22:52Z","timestamp":1532146972000},"page":"69-82","source":"Crossref","is-referenced-by-count":5,"title":["Radiolabelled leucocytes in human pulmonary disease"],"prefix":"10.1093","volume":"127","author":[{"given":"Neda","family":"Farahi","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge, Cambridgeshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrystalla","family":"Loutsios","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge, Cambridgeshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Tregay","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Cambridge School of 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