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In two models of asphyxial and asystolic cardiac arrest without resuscitation, we found that oedema develops shortly after anoxia secondary to terminal depolarizations and the abnormal entry of CSF. Oedema severity correlated with the availability of CSF with the age-dependent increase in CSF volume worsening the severity of oedema. Oedema was identified primarily in brain regions bordering CSF compartments in mice and humans. The degree of ex vivo tissue swelling was predicted by an osmotic model suggesting that anoxic brain tissue possesses a high intrinsic osmotic potential. This osmotic process was temperature-dependent, proposing an additional mechanism for the beneficial effect of therapeutic hypothermia. These observations show that CSF is a primary source of oedema fluid in anoxic brain. This novel insight offers a mechanistic basis for the future development of alternative strategies to prevent cerebral oedema formation after cardiac arrest.<\/jats:p>","DOI":"10.1093\/brain\/awab293","type":"journal-article","created":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T15:13:42Z","timestamp":1631891622000},"page":"787-797","source":"Crossref","is-referenced-by-count":47,"title":["Cerebrospinal fluid is a significant fluid source for anoxic cerebral oedema"],"prefix":"10.1093","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9020-3746","authenticated-orcid":false,"given":"Ting","family":"Du","sequence":"first","affiliation":[{"name":"Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center , Rochester, NY 14642, USA"},{"name":"School of Pharmacy, China Medical University , Shenyang 110122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5876-5397","authenticated-orcid":false,"given":"Humberto","family":"Mestre","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center , Rochester, NY 14642, USA"},{"name":"Department of Neuroscience, University of Rochester Medical Center , Rochester, NY 14642, USA"},{"name":"Department of Neurology, University of Pennsylvania , Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin T","family":"Kress","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center , Rochester, NY 14642, USA"},{"name":"Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen , 2200, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojun","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center , Rochester, NY 14642, USA"},{"name":"Department of Neurosurgery, the Fourth Affiliated Hospital of China Medical University , Shenyang 110032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda M","family":"Sweeney","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center , Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J","family":"Samson","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen , 2200, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin Kaag","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen , 2200, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian Nygaard","family":"Mortensen","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen , 2200, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2577-6783","authenticated-orcid":false,"given":"Peter A R","family":"Bork","sequence":"additional","affiliation":[{"name":"Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen , 2200, Copenhagen, Denmark"},{"name":"Department of Applied Mathematics and Computer Science, Technical University of Denmark, Richard Petersens Plads , 2800 Kgs. 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