{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:26:12Z","timestamp":1790727972855,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Inserm","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"CNRS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012946","name":"Sorbonne University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012946","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009810","name":"Paris Brain Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009810","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"European Union\u2019s Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007546","name":"ANSES","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007546","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In multiple sclerosis, while remarkable progress has been accomplished to control the inflammatory component of the disease, repair of demyelinated lesions is still an unmet need. Despite encouraging results generated in experimental models, several candidates favouring or promoting remyelination have not reached the expected outcomes in clinical trials. One possible reason for these failures is that, in most cases, during preclinical testing, efficacy was evaluated on histology only, while functional recovery had not been assessed.<\/jats:p>\n               <jats:p>We have generated a Xenopus laevis transgenic model Tg(mbp:GFP-NTR) of conditional demyelination in which spontaneous remyelination can be accelerated using candidate molecules. Xenopus laevis is a classic model for in vivo studies of myelination because tadpoles are translucent. We reasoned that demyelination should translate into loss of sensorimotor functions followed by behavioural recovery upon remyelination. To this end, we measured the swimming speed and distance travelled before and after demyelination and during the ongoing spontaneous remyelination and have developed a functional assay based on the visual avoidance of a virtual collision.<\/jats:p>\n               <jats:p>Here we show that alteration of these functional and clinical performances correlated well with the level of demyelination and that histological remyelination, assayed by counting in vivo the number of myelinating oligodendrocytes in the optic nerve, translated in clinical\u2013functional recovery. This method was further validated in tadpoles treated with pro-remyelinating agents (clemastine, siponimod) showing that increased remyelination in the optic nerve was associated with functional improvement.<\/jats:p>\n               <jats:p>Our data illustrate the potential interest of correlating histopathological parameters and functional\u2013clinical parameters to screen molecules promoting remyelination in a simple in vivo model of conditional demyelination.<\/jats:p>","DOI":"10.1093\/brain\/awad051","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T01:29:34Z","timestamp":1680226174000},"page":"2453-2463","source":"Crossref","is-referenced-by-count":10,"title":["Monitoring recovery after CNS demyelination, a novel tool to de-risk pro-remyelinating strategies"],"prefix":"10.1093","volume":"146","author":[{"given":"Esther","family":"Henriet","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elodie M","family":"Martin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Jubin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Langui","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelkrim","family":"Mannioui","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9631-4674","authenticated-orcid":false,"given":"Bruno","family":"Stankoff","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"},{"name":"AP-HP, Saint-Antoine Hospital , F-75012 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Lubetzki","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"},{"name":"AP-HP, GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arseny","family":"Khakhalin","sequence":"additional","affiliation":[{"name":"Bard College , 30 Campus Rd, Annandale-on-Hudson, NY 12504 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5484-2359","authenticated-orcid":false,"given":"Bernard","family":"Zalc","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, Inserm, CNRS, ICM-GH Piti\u00e9-Salp\u00eatri\u00e8re , F-75013 Paris , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"2023060100294111400_awad051-B1","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1016\/j.cub.2015.07.056","article-title":"CNS myelin sheath lengths are an intrinsic property of oligodendrocytes","volume":"25","author":"Bechler","year":"2015","journal-title":"Curr Biol"},{"key":"2023060100294111400_awad051-B2","first-page":"131","author":"Ranvier","year":"1878","journal-title":"Le\u00e7ons Sur l\u2019histologie Du Syst\u00e8me Nerveux. 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